Navigation Links
Pwo DNA Polymerase

for routine high fidelity amplification of up to 3.5 kb DNA targets Cat. No. 1 664 947 100 units
Cat. No. 1 664 955 2 x 250 units Description Pwo DNA polymerase was originally isolated from hyperthermophilic archaebacterium Pyrococcus woesei. The enzyme has a molecular weight of about 90 kD. It is a highly processive 5'3' DNA polymerase and possesses an 3'5' exonuclease activity also known as proofreading activity. The enzyme has no detectable 5'3' exonuclease activity. Pwo DNA polymerase exhibits increased thermal stability with a half life of greater than 2 h at 100C compared to Taq DNA polymerase with a half life of less than 5 min at this temperature. The inherent 3'5' exonuclease proofreading activity Pwo results in an over 18-fold increased fidelity of DNA synthesis compared to Taq DNA polymerase. Pwo DNA polymerase generated PCR products are blunt-ended and can therefore be used directly for blunt-end ligation without any pretreatment of the ends. For further information please download the Pwo DNA Polymerase
pack insert
. Application Fidelity of in vitro DNA polymerization is one of the most important subjects in PCR. For many applications of PCR, where a homogenous DNA population is analyzed (i.e. direct sequencing or restriction endonu-clease digestion), the mutations that are induced by the polymerase during PCR are of little concern. However if only a small amount of template DNA or RNA is used as starting mat erial and if after PCR single DNA molecules are analyzed, PCR artifacts can be a significant problem. Fidelity of DNA polymerization is for instance important for:
  • cloning of PCR products
  • study of allelic polymorphism in individual RNA transcripts (1, 2)
  • characterisation of the allelic stage of single cells (3) or single DNA molecules (4, 5)
  • characterisation of rare mutations in tissue (6)
  • characterisation of a population of cells in culture
Operating parameters
  • Thermal stability: Pwo DNA Polymerase is stable for >2 h at 95C
  • Divalent ion requirement: Mg2+ (standard concentration, 2.00 mM)
  • dNTP concentration: at least 200 M for each dNTP
    Caution: Lower deoxynucleotide concentrations can increase fidelity but can also activate the 3'5' exonuclease proofreading activity, which might degrade prime
Experimental results Key advantages
  • Maximum PCR fidelity, because the proofreading activity of Pwo DNA Polymerase excises misincorporated nucleotides
  • High PCR yield, because the polymerase is very resistant to the high denaturing temperatures used in PCR
  • Simplifies cloning of PCR products because it generates blunt-ended products which can be directly used f or blunt-end ligation
Note: Pwo DNA Polymerase cannot amplify targets longer than approx. 3.5 kb. For high fidelity amplification of longer templates use one of the Expand PCR Systems.
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Novel PCR Enhancing Factor Improves Performance of Pfu DNA Polymerase
2. Pfu DNA Polymerase as a Hot Start Alternative
3. PCR Performance Comparisons Between pfuturbo and Taq DNA Polymerases
4. pfuturbo DNA Polymerase: A High-Performance, High-Fidelity Enzyme Ideal for PCR Cloning
5. Unique DNA Polymerase Formulation Excels in a Broad Range of PCR Applications
6. Enhanced Amplification of Long Targets with PfuTurbo DNA Polymerase
7. High-Fidelity PCR with a Novel Polymerase Mixture
8. Optimizing pfuturbo DNA Polymerase Amplification Reactions with Perfect Match PCR Enhancer
9. Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR Performance
10. The Use of MasterTaq Polymerase for PCR with Humic Material-Contaminated DNA
11. HotStarTaq DNA Polymerase
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:2/8/2016)... BATH, England , February 8, 2016 ... Genetics Ltd ("Atlas Genetics" or the "Company"), the ultra-rapid Point-Of-Care ... approval to CE Mark its Chlamydia trachomatis (CT) test to ... requirements of the IVD Directive (98/79/EC), the CT test is ... --> --> The launch of the ...
(Date:2/5/2016)... DIEGO , Feb. 5, 2016 On ... region,s trusted information source for community, health and disaster ... Diego) will integrate to enhance care coordination and ... to the services they need and to better connect ... improve care.   San Diego ...
(Date:2/4/2016)...  Sangamo BioSciences, Inc. (NASDAQ: SGMO ), the ... Edward Lanphier , Sangamo,s president and chief executive officer, ... ZFP Therapeutic ® development programs and an overview ... on Thursday, February 11, 2016, at the Leerink Partners ... is being held in New York ...
(Date:2/4/2016)... MENLO PARK, Calif. , Feb. 4, 2016 /PRNewswire/ ... "Company"), a biopharmaceutical company focused on the development and commercialization ... at the 18 th Annual BIO CEO & ... a.m. EST in New York, NY . ... will provide an update on the ongoing clinical trial of ...
Breaking Biology Technology:
(Date:2/4/2016)... , Feb. 4, 2016 The field ... one of the most popular hubs of the ... and other huge studies of human microbiota, have ... few years, the microbiome space has literally exploded ... research. This report focuses on biomedical aspects ...
(Date:2/2/2016)... , Feb. 2, 2016  BioMEMS ... are primarily focused on medical screening and ... point-of-care parameters. Wearable devices that facilitate and ... freedom of movement are being bolstered through ... human biomedical signal acquisition coupled with wireless ...
(Date:2/2/2016)... 2, 2016  Based on its recent analysis ... recognizes US-based Intelligent Retinal Imaging Systems (IRIS) with ... for New Product Innovation. IRIS, a prominent cloud-based ... America , is poised to set the ... retinopathy market. The IRIS technology presents superior price-performance ...
Breaking Biology News(10 mins):