Navigation Links
Purified Single-Stranded M13 DNA for Automated Sequencing


A streamlined and reliable method to obtain high-quality single-stranded DNA

Jeff Braman Scott Basehore
Stratagene

The StrataPrep M13 DNA purification kit allows easy, quick, and reliable purification of bacteriophage single-stranded DNA. The yield of single-stranded DNA recovered from phage lysate exceeds the yield obtained with the leading commercial silica-gel-based (SGB) M13 DNA purification method. The resulting high-purity DNA is ideal for automated sequencing applications.

M13 DNA is a preferred template for many sequencing projects.1-5 For many years, the standard laboratory protocol used to purify M13 DNA relied upon polyethylene glycol (PEG)-NaCl precipitation of phage particles, phenol-chloroform extraction to remove phage coat proteins, and ethanol precipitation to concentrate the DNA.6 This method is time-consuming, employs hazardous chemicals, and is difficult to apply to more than a few samples.

However, with the StrataPrep M13 DNA purification kit, isolating high-purity M13 DNA is possible without these cumbersome steps because the procedure is streamlined: Collect M13 phage particles on the unique glass fiber matrix contained in a StrataPrep spin cup;7 add a chaotropic salt solution to the spin cup so the phage coat protein denatures and binds the liberated single-stranded DNA to the glass matrix; centrifuge-wash the phage DNA to remove contaminating protein and salt; and elute the DNA from the spin cup with a low ionic strength buffer. The concentration of the highly purified DNA that results is ideal for automated sequencing applications.

High Yield Recovery of Single-Stranded DNA

The yield of single-stranded DNA from StrataPrep M13 method of Phagescript lysate (a modified M13 bacteriophage8), was at least twice
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Automated Fluorescent-Tag Cycle Sequencing
2. CastAway Precast Gels for Rapid Automated DNA Sequence Analysis
3. The DIG System Nonradioactive Automated High-Throughput In Situ Hybridization: a Powerful Tool for Functional Genomics Research
4. Agilent 2100 Bioanalyzer Automated Analysis System
5. Automated RNA Amplification for Arrays
6. Quantitation of DNA for Automated Sequencing Using the VersaFluor Fluorometer
7. Performance Comparison of the Experion Automated Electrophoresis System and a Competing Automated System for Protein Analysis, Rev A
8. Total RNA Purification from Cultured Cells Using the ABI PRISM 6700 Automated Nucleic Acid Workstation and Total RNA Lysis Reagents
9. DNA Damage and Repair - Quantification of Sub-Cellular Events Using Automated Confocal Imaging
10. Small-Molecule Quantitation Using Automated Single-Shot Averaging MALDI-TOF Mass Spectrometry
11. Automated Dissolution of Prednisone with the MultiDose G3
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Purified Single Stranded DNA for Automated Sequencing

(Date:12/24/2014)... December 23, 2014 The ... such as its definition, classification, application and ... specification, manufacturing process, and product cost structure. ... applications. The analysis also covers upstream raw ... industry development trend and proposals. In the ...
(Date:12/24/2014)... 2014 Vermillion, Inc. (Nasdaq:   VRML), ... announced today that investors including Oracle Investment Management, ... and several Vermillion directors have agreed to purchase ... common stock and warrants to purchase unregistered shares ... Under the terms of the ...
(Date:12/22/2014)... Brussels (PRWEB) December 22, 2014 The ... the global leader of technical training across the life ... Clinical Data Management (SCDM) to provide the organization's ... certification programs —providing access to the more than 350 ... SCDM members with 10% off when registering for a ...
(Date:12/22/2014)... Dec. 22, 2014  ( www.competitivehealth.com ) — Competitive ... medical bill review and advocacy service, has signed an ... WellCard Savings discount health services marketplace. 63% ... more than they expected to pay. As part of ... costs, WellCard Savings is pleased to offer medical bill ...
Breaking Biology Technology:Worldwide Methyl Mercaptan Market 2015-2020 Forecasts on Development & Trends Now Available at ReportsnReports.com 2Vermillion Announces Equity Financing of up to $18.9 Million; Suspends ATM Program 2Vermillion Announces Equity Financing of up to $18.9 Million; Suspends ATM Program 3Vermillion Announces Equity Financing of up to $18.9 Million; Suspends ATM Program 4Vermillion Announces Equity Financing of up to $18.9 Million; Suspends ATM Program 5CfPIE Announces Partnership with the Society for Clinical Data Management 2CfPIE Announces Partnership with the Society for Clinical Data Management 3WellCard Savings Announces CoPatient Medical Bill Review Service for Members 2
... chemists have developed a new way to make transistors ... integrated into high-performance computer chips to increase their speed ... chips when transistors are packed together tightly. , For ... Dai, the J. G. Jackson and C. J. Wood ...
... research institute to aid in successful mid-career changes, ... http://www.biocareercenter.com ) an online job board and careers tool ... who has developed Career Navigator -- a new product ... their fields with success --,to launch this new online ...
... May 28 The automation of,transaction-based processing ... in the United States. Foresight Corporation of ... and scale of installations of Transaction,Insight(R) - ... Transaction automation techniques enable healthcare organizations ...
Cached Biology Technology:Carbon nanoribbons could make smaller, speedier computer chips 2Online Career Service Turns Focus to 'Switching Gears' 2New Trend Sweeping Nation's Health Plans: Transaction Automation 2
(Date:11/21/2014)... , November 18, 2014 According ... Market by Systems (Video, RFID, Access Control, Intrusion Detection, ... Hotels, Banks, Government), Component Service Geography - Global Forecasts ... Market is projected to be around $25 Billion in ... 2020, growing at a CAGR of 8.69%. ...
(Date:11/21/2014)... , Nov. 20, 2014 Strict laws against ... are piloting the North American and European automotive sector ... growing, gesture recognition systems that are intuitive and able ... in the industry. New analysis from ... Recognition Market in Europe and ...
(Date:11/21/2014)... 20, 2014   Atmel® Corporation (NASDAQ: ... and touch technology solutions, today launched the industry,s first ... the widest V cc range from 1.7V to ... faster I 2 C bus communication speeds, and are ... making them ideal for consumer, industrial, computer, and medical ...
Breaking Biology News(10 mins):Industrial Security Systems Market Worth $38 Billion by 2020 2Industrial Security Systems Market Worth $38 Billion by 2020 3Gesture Recognition Intensifies with the Rise of In-car Smartphone Integration, Says Frost & Sullivan 2Gesture Recognition Intensifies with the Rise of In-car Smartphone Integration, Says Frost & Sullivan 3Gesture Recognition Intensifies with the Rise of In-car Smartphone Integration, Says Frost & Sullivan 4Atmel Launches Industry's First Wide-V(cc) Low-Power Temperature Sensor Family 2Atmel Launches Industry's First Wide-V(cc) Low-Power Temperature Sensor Family 3Atmel Launches Industry's First Wide-V(cc) Low-Power Temperature Sensor Family 4
... severed nerves could result in patients recovering in days or ... cellular mechanism similar to that used by many invertebrates to ... in the Journal of Neuroscience Research . "We ... minutes so that the behavior they control can be partially ...
... that make up half the biomass in the oceans ... over, mostly remain enigmatic. A few abundant groups have ... of the rest remain mysterious. Understanding how the ... is like trying to understand the solar system when ...
... fertile, excite our most basic urges, and as scientists have ... from women. But how? Now a team of scientists ... many genes influenced by the male and female sex hormones ... of male and female behaviors in mice. The UCSF ...
Cached Biology News:New procedure repairs severed nerves in minutes, restoring limb use in days or weeks 2Scientists coax shy microorganisms to stand out in a crowd 2Scientists coax shy microorganisms to stand out in a crowd 3Male and female behavior deconstructed 2Male and female behavior deconstructed 3Male and female behavior deconstructed 4
... polyclonal antibody raised against a ... Immunogen: SLC22A3 (NP_068812, ... partial recombinant protein with GST ... Number: NM_021977 ...
... The Amplifluor UniPrimer-fluorescein is a hairpin ... transfer pair (fluorescein and non-fluorescent quencher) ... The UniPrimer is identical to ... Universal Detection System (S7901) and is ...
... Disposable. Virgin, optically clear polystyrene with ... alphanumeric coordinates for cell counting. 100% ... is a new product number, created ... If showing no availability yet, please ...
... The Rotofor purification system with PowerPac 3000 ... protein separation and purification by liquid-phase isoelectric ... mini (18 ml) and standard (60 ml) ... electrode chambers, PowerPac 3000 power supply, 10 ...
Biology Products: