Navigation Links
Purification of Two Isoforms of Pyruvate Kinase from Nordic Krill Using an UNO Q1 Column, Rev A

applied to an UNO Q-1 column (Bio-Rad catalog # 720-0001, see Figure 1). PK I and PK II were well-separated by this procedure at pH 8.0. The previous protocol, using a popular high resolution beaded support, required the use of pH 9.0 buffer to prepare the PK I species, at which pH the enzyme is less stable. The addition of PEP as a substrate stabilizing agent was also required in this pH 9.0 system but not for the UNO-based system. The purified enzyme obtained by this purification process was used to determine that the PK I of Nordic krill does, indeed, exhibit altered physicochemical and kinetic properties with the seasons and may contribute to the temperature adaptations of this species.

Reference
Vetter, R-A.H., and Buchholz, F., Comp. Biochem. Physiol., 116A, 110 (1997).

Data courtesy of Dr. Rolph-Achim Vetter, Biologische Anstalt Helgoland, Meeresstation.


back to top
'"/>

Source:


Page: All 1 2

Related biology technology :

1. High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli
2. Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels
3. Yeast Protein Production System Features High Yields and One-Step Purification
4. Expression and Purification of Recombinant Proteins That Have Native Amino Acid Sequence
5. Easy Cosmid DNA Purification with StrataPrep Kits
6. MinElute PCR Purification Kit
7. QIAquick PCR Purification Kit
8. Microarray Target Purification Kit and the Microarray Target Synthesis Kit (T7)
9. Double-Affinity Purification of GST-His Tagged Proteins Using Vivapure 8-to-96 Well Cobalt-Chelate Kit
10. Ultra-Fast Purification of Small Nucleic Acids
11. RNAClean - RNA & cDNA in vitro Reaction Purification
Post Your Comments:
(Date:6/3/2015)... Typical racks for laboratory freezers are generally ... often taking up unnecessary room in already crowded freezers. ... Box Freezer Racks offer a customizable storage solution ... boxes and freezers. , “These racks are perfect ... multiple people because users can configure freezer space to ...
(Date:6/3/2015)... Green Earth Energy has restructured their ... the RCBC Waste to Energy system. Paul Douglas ... family business, http://www.greenearthwaste.com which has been operating ... recycling to their waste collection services in 2012 and ... Fortune 500 companies with manufacturing plants in West Tennessee. ...
(Date:6/3/2015)... REYKJAVIK, Iceland , June 3, 2015   ... genome to improve health worldwide, today announced that it ... to provide clinical interpretation of the whole genome sequences ... of the first phase of the 100,000 Genomes Project ... WuXi NextCODE will deploy its unique database ...
(Date:6/3/2015)... June 03, 2015 Global Stem ... Inc. have announced plans to conduct Romania’s first ever ... Through an alliance with Miami-based Stem Cell Training, Inc., ... the Adipose and Bone Marrow Stem Cell Training Course ... reconstructive plastic surgery specialist practicing in Bucharest and Tel ...
Breaking Biology Technology:Freezer Space Can Now Be Configured Your Way with New Modular Freezer Racks 2WuXi NextCODE Wins Genomics England Clinical Interpretation Tender in both Rare Disease and Cancer 2Global Stem Cells Group Announces First Stem Cell Training Course to Take Place in Romania 2Global Stem Cells Group Announces First Stem Cell Training Course to Take Place in Romania 3
... Jersey, October 29, Rosetta Genomics Ltd. (Nasdaq: ROSG ... announced today its,management will present at Oppenheimer 19th ... the Waldorf-Astoria Hotel in New York,City., About ... occurring, small,RNAs that act as master regulators and ...
... /Xinhua-PRNewswire/ -- Sinovac Biotech Ltd. (Amex:,SVA), a vaccine-focused ... scheduled to present at two upcoming investor conferences, ... York City and the,Credit Suisse Asian Healthcare Conference ... Financial Manager, is scheduled to present on,Tuesday, November ...
... Osteotech, Inc.,(Nasdaq: OSTE ), a leader ... healing, announced today that it has received 510(k) ... ("FDA") for its next,generation grafting material. Osteotech received ... substitute, and as a bone void filler, for ...
Cached Biology Technology:Rosetta Genomics to Present at Oppenheimer's 19th Annual Healthcare Conference 2Rosetta Genomics to Present at Oppenheimer's 19th Annual Healthcare Conference 3Sinovac to Participate in Two Upcoming Investor Conferences 2Osteotech Receives FDA Clearance for its Next Generation Grafting Material 2
(Date:5/27/2015)... , May 27, 2015   ... medication safety systems and image documentation solutions ... of comprehensive, technologically-advanced automation solutions, is pleased ... integration. The Codonics® Safe Label System® (SLS), ... in conjunction with the Omnicell Anesthesia Workstation, ...
(Date:5/22/2015)... According to a new market research report ... by Software (Middleware, Databases), by Hardware (Cameras, Integrated Devices), ... - Global Forecast to 2020", published by MarketsandMarkets, Facial ... to $6.19 Billion by 2020, at a CAGR of ... and   43 Figures spread through 140 Pages and ...
(Date:5/20/2015)... OXFORD, Conn. , May 20, 2015 ... "Company") announces that its wholly owned subsidiary, 3D-ID LLC, ... contractor under Team Battelle for the biometrics technology portion ... Unrestricted Suite Contract from the Department of the Army. ... facial recognition products developed for government, law enforcement and ...
Breaking Biology News(10 mins):Codonics and Omnicell Expand Partnership 2Codonics and Omnicell Expand Partnership 3Codonics and Omnicell Expand Partnership 4Facial Recognition Market Worth $6.19 Billion by 2020 2Facial Recognition Market Worth $6.19 Billion by 2020 3NXT-ID, Inc Announces Participation as Member of Team Battelle for TIES Contract Award 2NXT-ID, Inc Announces Participation as Member of Team Battelle for TIES Contract Award 3
... Francisco have launched a groundbreaking study to determine whether ... the most common congenital chest deformity, in the same ... chest, which is known medically as pectus excavatum, is ... to the breastbone. The deformed cartilage pulls the breastbone ...
... recurrent cancer of the central nervous system that occurs primarily ... a clinical trial at the University of California, San Francisco. ... is always fatal. Findings from a group of 12 ... effective in stimulating the patient’s immune system to attack ...
... that chimpanzees from West Africa were cracking nuts with ... years ago. The result suggests chimpanzees developed this behaviour ... was a trait inherited from our common ancestor. Julio ... the Noulo site in Cote d'Ivoire, the only known ...
Cached Biology News:Study to explore using magnets to correct 'sunken chest' 2Study to explore using magnets to correct 'sunken chest' 3UCSF brain tumor vaccine trial shows promising results 2UCSF brain tumor vaccine trial shows promising results 3The chimpanzee stone age 2