Navigation Links
Purification of Phosphoinositide 3-Kinase With UNO Columns


Polyphosphoinositides and their metabolites are important intracellular signals involved in the responses to a number of hormones and growth factors.The discovery of a phosphatidylinositol kinase that phosphorylates PI at the 3 position of the inositol ring uncovered a new pathway of PI metabolism and potential intracellular signals.

Phosphoinositide 3-kinase has been purified to homogeneity from rat liver and bovine thymus. The enzyme from rat liver is a heterodimer of 110 and 85 kDa proteins.Two forms of the 110 kDa protein exist, and have similar but not identical amino acid sequences. Recent studies have suggested that the 110 kDa protein is the catalytic protein and that the 85 kDa protein plays a regulatory role.

Phosphoinositide 3-kinase has proven difficult to purify in an active form when overexpressed in various expression systems. Consequently, for continued biochemical studies of this phosphoinositide pathway, a routine purification protocol for phosphoinositide 3-kinase from rat liver has been established.The protocol involves multiple steps including size exclusion, hydroxyapatite, and ion-exchange chromatography.

The chromatograms (Figures 1 and 2) illustrate the use of an UNO Q1 column to partially purify a cytosolic phosphoinositide 3-kinase from a rat liver homogenate at a flow rate 5-fold higher than previously used with a conventional monodispersed beaded media.

Both the overall resolution and protein activity profile are maintained at the higher flow.


Acknowledgement
Data courtesy of Dr.A. Couvillon, Beth Israel Deaconess Medical Center, Boston, MA.


FPLC is a
'"/>

Source:


Page: All 1 2

Related biology technology :

1. High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli
2. Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels
3. Yeast Protein Production System Features High Yields and One-Step Purification
4. Expression and Purification of Recombinant Proteins That Have Native Amino Acid Sequence
5. Easy Cosmid DNA Purification with StrataPrep Kits
6. MinElute PCR Purification Kit
7. QIAquick PCR Purification Kit
8. Microarray Target Purification Kit and the Microarray Target Synthesis Kit (T7)
9. Double-Affinity Purification of GST-His Tagged Proteins Using Vivapure 8-to-96 Well Cobalt-Chelate Kit
10. Ultra-Fast Purification of Small Nucleic Acids
11. RNAClean - RNA & cDNA in vitro Reaction Purification
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Purification Phosphoinositide Kinase With UNO Columns

(Date:3/31/2015)... Diasome Pharmaceuticals, Inc. ( http://www.diasome.com ) has received ... States patent has recently been allowed for the Company’s ... “Lipid Construct for Delivery of Insulin to a Mammal,” ... the use of Diasome’s proprietary Hepatocyte Directed Vesicles (“HDV”) ... effectively reach the liver’s metabolic cells, called hepatocytes. , ...
(Date:3/30/2015)... PMG Research, Benchmark Research, and Miami ... of VaxCorps, a network of highly experienced, geographically ... of vaccine trials in healthy adult, elderly, and ... conducted over 500 phase I-III vaccine clinical trials, ... as well as a vast array of other ...
(Date:3/30/2015)... , March 30, 2015  NuGene International, Inc. ... corporation, NuGene BioPharma, Inc. has added another internationally ... Dr. Guan is an internationally recognized researcher in ... college education at Peking University, China ... from the University of North Carolina, Chapel Hill. ...
(Date:3/30/2015)... 2015 CASI Pharmaceuticals, Inc. (Nasdaq: CASI ... and commercialization of innovative therapeutics addressing cancer and other ... commercial focus on China , announced ... has approved the Company,s application to conduct a Phase ... (OCCC) patients for its proprietary drug candidate ENMD-2076.  ...
Breaking Biology Technology:Diasome Pharmaceuticals, Inc. Announces Notice of Allowance from US PTO for Liver Targeting System for Insulin 2PMG Research announces formation of VaxCorps with two industry-leading vaccine site networks 2UCI Professor Zhibin Guan Joins NuGene Advisory Board 2UCI Professor Zhibin Guan Joins NuGene Advisory Board 3CASI Pharmaceuticals Receives Approval From CFDA For Phase 2 Clinical Trial In China With ENMD-2076 For Ovarian Clear Cell Carcinoma 2CASI Pharmaceuticals Receives Approval From CFDA For Phase 2 Clinical Trial In China With ENMD-2076 For Ovarian Clear Cell Carcinoma 3CASI Pharmaceuticals Receives Approval From CFDA For Phase 2 Clinical Trial In China With ENMD-2076 For Ovarian Clear Cell Carcinoma 4CASI Pharmaceuticals Receives Approval From CFDA For Phase 2 Clinical Trial In China With ENMD-2076 For Ovarian Clear Cell Carcinoma 5
... Medicine , Susan Huang and colleagues describe the use ... by integrating two freely available software packages, to identify ... data from patients admitted to a 750-bed academic medical ... authors found that the tool identified a number of ...
... ... a US report on Fermenters market. The US market for fermenters is projected to reach ... demand for biological drugs worldwide, and growing use of disposable or single-use fermenters. , ... San Jose, California (PRWEB) February 22, ...
... special ink infused with nanoparticles, Stanford engineer Yi Cui ... lightweight paper batteries and supercapacitors (which, like batteries, store ... well as stretchable, conductive textiles known as "eTextiles" ... of ordinary paper or fabric. While the technology ...
Cached Biology Technology:Tool identifies infection clusters; rate of T cell drop not helpful in decision to start treating HIV 2Tool identifies infection clusters; rate of T cell drop not helpful in decision to start treating HIV 3Tool identifies infection clusters; rate of T cell drop not helpful in decision to start treating HIV 4US Fermenters Market to Reach $180 million by 2015, According to New Report by Global Industry Analysts, Inc. 2US Fermenters Market to Reach $180 million by 2015, According to New Report by Global Industry Analysts, Inc. 3Nanotechnology sparks energy storage on paper and cloth 2Nanotechnology sparks energy storage on paper and cloth 3
(Date:3/26/2015)... 26, 2015 The Granite Club, ... and athletic club, today announced it has implemented a ... of movement for members and staff, while restricting access ... process, we selected FST,s IMID Access system because it ... our members and staff, in addition to unparalleled security," ...
(Date:3/24/2015)... 2015   NexID Biometrics LLC, whose spoof-mitigation ... today announced the beginning of shipments of version 2.0 ... The company, based in Potsdam, N.Y. ... Expo, which began here today at the Walter E. ... of its SDK boosts the accuracy rate range to ...
(Date:3/23/2015)...   HOYOS Labs , a leading global authentication ... the Company will demonstrate multiple biometrics-based technologies under its ... Connect:ID on March 23 through 25, 2015, in ... the IEEE Biometric Open Protocol Standard; free biometrics-based consumer ... control system. BOPS was invented by HOYOS Labs and ...
Breaking Biology News(10 mins):Granite Club First Private Club in North America to Implement FST Biometrics Secure Access Solution, as Part of Multi-million Dollar Expansion 2NexID Begins Shipments Of Version 2.0 Fake-Finger-Detection Solution 2HOYOS Labs to Showcase Host of Biometrics Technologies at Connect:ID in Washington, D.C. 2HOYOS Labs to Showcase Host of Biometrics Technologies at Connect:ID in Washington, D.C. 3
... National Institute of Standards and Technology (NIST) have developed ... governing a wide variety of cellular functions that may ... cells. The NIST technique measures changes in a ... that expresses the favorability of reactions in which molecules ...
... ANN ARBOR, Mich. We are all aware of the ... and how do we metabolize it? Research at the ... Biology Laboratory, and institutions in Canada and Sweden, has begun ... carbohydrates found in fruits and vegetables. Trillions of bacteria ...
... algae, rosemary and monk fruit could soon replace synthetic ... BHT and aspartame that label-conscious grocery shoppers are increasingly ... sweeteners and preservatives to naturally derived ones, and could ... & Engineering News , the weekly newsmagazine of the ...
Cached Biology News:New NIST method evaluates response to oxidation in live cells 2U-M scientists & colleagues investigate the fiber of our being 2
Mouse Aminopeptidase P2/XPNPEP2 Affinity Purified PAb Protein Family: Aminopeptidases, Metalloproteases...
... 53 new primer sets added in Dec ... than 3 hours , Detection from ... , Specific primers available for hundreds ... either real-time or end-point RT-PCR , ...
AGAROSE PREP, 50G, 1 EA. Category: Genephor Precast Gels....
in vitro Translation, Accessory Products...
Biology Products: