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Purification of Murine IgG1 Using UNOsphere S and CHT ,,, Ceramic Hydroxyapatite Chromatography, Rev A

y to processes using protein A chromatography (data not shown).


Acknowledgement
Special thanks to Paul Yazaki, PhD, Beckman Research Institute of the City of Hope National Medical Center, Duarte, CA, for his numerous contributions to this project.


References
Ford CH et al., Affinity purification of novel bispecific antibodies recognising carcinoembryonic antigen and doxorubicin, J Chromatogr B Biomed Sci and Appl 754, 427435 (2001)

Godfrey MAJ et al., Assessment of the suitability of commercially available SpA affinity solid phases for the purification of murine monoclonal antibodies at process scale, J Immunol Methods 160, 97105 (1993)

Jiskoot W et al., Two-step purification of a murine monoclonal antibody intended for therapeutic application in man. Optimisation of purification conditions and scaling up, J Immunol Methods 124, 143156 (1989)

Shadle PJ et al., Antibody purification, US patent 5,429,746 (1995)


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