Navigation Links
Purification of Murine IgG1 Using UNOsphere S and CHT ,,, Ceramic Hydroxyapatite Chromatography, Rev A

3- ions in its spherical and ceramic structure, provides excellent resolution and selectivity for protein isolation.


Methods and Results
The BioLogic DuoFlow workstation was used to develop the processes. An analytical column (0.7 x 5 cm) was loaded with UNOsphere S support for small-scale process development. A preparative column (2.2 x 20 cm) was used in process scale-up. All SDS-PAGE was performed using Ready Gel precast gels, the Criterion cell, and the PowerPac 3000 power supply. Ceramic hydroxyapatite (Type I, 10 m and 20 m) media were used for this study.

Small-Scale Process Development
UNOsphere S chromatography was used as the capture step for murine IgG1 isolation. The binding capacity of UNOsphere S support for murine IgG1 was optimized at pH values from 7.0 to 4.0. Decreasing the pH in the cell culture increased the binding capacity of UNOsphere S support for murine IgG1 (data not shown). The murine IgG1 was stable for at least 6 hr in buffer at pH 4.0 (data not shown), so the cell culture was adjusted to pH 4.0. The cell culture was then loaded onto the UNOsphere S column as described in Figure 1. The murine IgG1 was eluted in the NaCl gradient. The fractions were analyzed by SDS-PAGE (Figure 2). It was found that Phenol Red and some contaminating proteins were removed in the flow-through pool by UNOsphere S chromatography.

For ceramic hydroxyapatite chromatography, the murine IgG1 fractions were pooled and diluted 5-fold with 1 mM sodium phosphate buffer, pH 6.8. The murine IgG1 elution pool from the UNOsphere S column could not be loaded directly on the CHT supports bec
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli
2. Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels
3. Yeast Protein Production System Features High Yields and One-Step Purification
4. Expression and Purification of Recombinant Proteins That Have Native Amino Acid Sequence
5. Easy Cosmid DNA Purification with StrataPrep Kits
6. MinElute PCR Purification Kit
7. QIAquick PCR Purification Kit
8. Microarray Target Purification Kit and the Microarray Target Synthesis Kit (T7)
9. Double-Affinity Purification of GST-His Tagged Proteins Using Vivapure 8-to-96 Well Cobalt-Chelate Kit
10. Ultra-Fast Purification of Small Nucleic Acids
11. RNAClean - RNA & cDNA in vitro Reaction Purification
Post Your Comments:
(Date:7/2/2015)... July 2, 2015 College of DuPage students ... are gaining invaluable real-world experiences while serving biophysics internships ... The students will be working as a ... group at IIT. The internship came about from ... , Physics; Dr. Kathy Finan , Biology; and ...
(Date:7/2/2015)... ... ... Sleepless nights will become obsolete as Luzi , a sophisticated smart ... June 23. Sound sleepers and the sleep deprived both will love Luzi for its ... integration of the Bluetooth speaker, alarm clock and ability to charge various devices, including ...
(Date:7/1/2015)... and PARIS , July ... and Genective, key developer of biotech crops, today ... of new generations of insect control traits. ... to counter the realities of advancing insect resistance. ... AgBiome,s unique insect control technology with Genective,s capability ...
(Date:7/1/2015)... and BANGALORE, India , July ... profiling company that uses next generation sequencing technology to ... Scott A. Storrer as chief executive officer. In ... will be responsible for all strategy, business expansion and ... who has served as Strand,s chairman and CEO since ...
Breaking Biology Technology:COD Students Participate in Prestigious Summer Internships 2Luzi Smart Lamp Recently Launched Indiegogo Campaign 2Luzi Smart Lamp Recently Launched Indiegogo Campaign 3AgBiome and Genective collaborate to create novel insect-resistant crops 2Strand Life Sciences Names Scott A. Storrer CEO as Company Continues Global Expansion with Focus on Precision Medicine Product 2Strand Life Sciences Names Scott A. Storrer CEO as Company Continues Global Expansion with Focus on Precision Medicine Product 3
... has named Todd Creech as Chief Financial Officer and ... Creech has been fulfilling both positions as a consultant ... http://photos.prnewswire.com/prnh/20110214/FL47222LOGO ) "Todd comes to ... leadership roles and has demonstrated great success in helping ...
... Even with radical advances in medicine and technology ... penile extender system is still the most effective ... QuickExtenderPro.com, the leading provider of penis extender ... With the emergence of the penile extender, procedures such ...
... EXTON, Pa., Feb. 11, 2011 ViroPharma Incorporated (Nasdaq: ... chief executive officer of ViroPharma, will present at the Leerink ... ET on Thursday, February 17, 2011.  The conference is being ... ViroPharma,s presentations will be webcast live for investors through ...
Cached Biology Technology:Todd A. Creech Named Chief Financial Officer and Vice President of Business Development for SARcode Corporation 2Todd A. Creech Named Chief Financial Officer and Vice President of Business Development for SARcode Corporation 3QuickExtenderPro.com Penis Extender Device Engineered for Quick, Safe and Effective Alternative to Penis Enlargement Surgery 2ViroPharma to Participate in the Leerink Swann Hot Topics in Therapeutics Roundtable Conference 2
(Date:6/16/2015)... GOTHENBURG, Sweden , June 16, 2015 ... 2015 from previously communicated that it will amount to at ... 390 MSEK. Due to the rapid increase in market growth ... from the previously communicated guidance that revenue for 2015 will ... 2015 will amount to approximately 2,200 MSEK. Due ...
(Date:6/12/2015)... DALLAS , June 12, 2015  Securus ... justice technology solutions for public safety, investigation, corrections ... issued its patent to electronically capture, analyze and ... various facial recognition methods. This system ... authorized by an enrollment and subsequent sign-on process ...
(Date:6/9/2015)... 09, 2015 Research and Markets  ( ... "Gesture Recognition & Touchless Sensing Market by ... & Others), Product (Biometric & Sanitary Equipment) & ... report to their offering. The total ... to reach $ 23.55 Billion by 2020 at ...
Breaking Biology News(10 mins):Fingerprint Cards Raises the Revenue Guidance for Q2 2015 and for the Full Year 2015 2Fingerprint Cards Raises the Revenue Guidance for Q2 2015 and for the Full Year 2015 3Securus Announces Patent Issued for Authorized Presence Verification During Video Visitation 2Global Gesture Recognition & Touchless Sensing (Technology, Application & Products) Market 2015-2020 2
... 2010 In findings that advance scientists, understanding of a ... Research Institute has shed light on a mechanism that enables ... diseases. The findings were published in an advance, online ... May 9, 2010. "This study is likely to motivate ...
... a member of the IOF Board and long standing ... named the recipient of the prestigious Pierre Delmas Award. ... (IOF) in memory of Pierre D. Delmas, IOF,s renowned ... the Foundation and osteoporosis advocacy worldwide. The ...
... around the damage caused by a heart attack survived in ... in a mouse model, researchers at The University of Texas ... Circulation Research . The study, with researchers at ... innovative imaging techniques developed by researchers at MD Anderson to ...
Cached Biology News:Scripps research scientists shed light on potential treatment for Gaucher's disease 2Scripps research scientists shed light on potential treatment for Gaucher's disease 3Scripps research scientists shed light on potential treatment for Gaucher's disease 4Transplanted adult stem cells provide lasting help to injured hearts 2Transplanted adult stem cells provide lasting help to injured hearts 3Transplanted adult stem cells provide lasting help to injured hearts 4
...
... from different Cynomolgus monkey normal tissues ... available upon request. Customers can ... whole set, and pay 70% of ... Dr. P Set products are for ...
... consists of AmnioMAX-C100 Basal ... and AmnioMAX-C100 Supplement (Cat. ... complete with antibiotics and ... requires no further supplementation ...
Anti-Dynamin Monoclonal Antibody Description: 100 g Research Focus: neuroscience Storage: -20C Shipping Temperature: 4C...
Biology Products: