Navigation Links
Purification of Murine IgG1 Using UNOsphere S and CHT ,,, Ceramic Hydroxyapatite Chromatography, Rev A

3- ions in its spherical and ceramic structure, provides excellent resolution and selectivity for protein isolation.


Methods and Results
The BioLogic DuoFlow workstation was used to develop the processes. An analytical column (0.7 x 5 cm) was loaded with UNOsphere S support for small-scale process development. A preparative column (2.2 x 20 cm) was used in process scale-up. All SDS-PAGE was performed using Ready Gel precast gels, the Criterion cell, and the PowerPac 3000 power supply. Ceramic hydroxyapatite (Type I, 10 m and 20 m) media were used for this study.

Small-Scale Process Development
UNOsphere S chromatography was used as the capture step for murine IgG1 isolation. The binding capacity of UNOsphere S support for murine IgG1 was optimized at pH values from 7.0 to 4.0. Decreasing the pH in the cell culture increased the binding capacity of UNOsphere S support for murine IgG1 (data not shown). The murine IgG1 was stable for at least 6 hr in buffer at pH 4.0 (data not shown), so the cell culture was adjusted to pH 4.0. The cell culture was then loaded onto the UNOsphere S column as described in Figure 1. The murine IgG1 was eluted in the NaCl gradient. The fractions were analyzed by SDS-PAGE (Figure 2). It was found that Phenol Red and some contaminating proteins were removed in the flow-through pool by UNOsphere S chromatography.

For ceramic hydroxyapatite chromatography, the murine IgG1 fractions were pooled and diluted 5-fold with 1 mM sodium phosphate buffer, pH 6.8. The murine IgG1 elution pool from the UNOsphere S column could not be loaded directly on the CHT supports bec
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli
2. Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels
3. Yeast Protein Production System Features High Yields and One-Step Purification
4. Expression and Purification of Recombinant Proteins That Have Native Amino Acid Sequence
5. Easy Cosmid DNA Purification with StrataPrep Kits
6. MinElute PCR Purification Kit
7. QIAquick PCR Purification Kit
8. Microarray Target Purification Kit and the Microarray Target Synthesis Kit (T7)
9. Double-Affinity Purification of GST-His Tagged Proteins Using Vivapure 8-to-96 Well Cobalt-Chelate Kit
10. Ultra-Fast Purification of Small Nucleic Acids
11. RNAClean - RNA & cDNA in vitro Reaction Purification
Post Your Comments:
(Date:2/27/2015)... Research and Markets ( http://www.researchandmarkets.com/research/p2ddnr/global_market ) has ... Trypsin" report to their offering. , ... comprehensive data on Trypsin globally and regionally ( Europe ... America , Latin America etc.). ... This report focuses on three primary areas; manufacture ...
(Date:2/27/2015)... Brighton, Illinois (PRWEB) February 27, 2015 ... Matt Sims, General Manager of State Line Farms, received ... Award recognizes a producer, under the age of 35, ... , "It is an honor to win the Tomorrow's ... to discovering who won the Top Producer Award and ...
(Date:2/26/2015)... Feb. 26, 2015 On behalf of biotech ... companies, Wilentz, Goldman & Spitzer, P.A. (Wilentz) ... against Genewiz Inc. in excess of $10 million. The ... trade secrets, and that it improperly hired one or ... denying motions for judgment notwithstanding the verdict, a new ...
(Date:2/26/2015)... PARK, Calif. , Feb. 26, 2015 ... released the latest version of the Governance Portal ... accomplish governance, risk and compliance (GRC) tasks across multiple ... also been updated to help customers better align their ... of the Treadway Commission ) requirements. In response to ...
Breaking Biology Technology:Global Market Report of Trypsin 2014-2018 2Matt Sims Receives the 2015 Tomorrow’s Top Producer Horizon Award 2Wilentz Client GenScript Awarded $10 Million in Trade Secrets Case Against Genewiz 2Wilentz Client GenScript Awarded $10 Million in Trade Secrets Case Against Genewiz 3Protiviti Governance Portal Helps Companies Drive Governance, Risk and Compliance Productivity Across Multiple Business Units 2Protiviti Governance Portal Helps Companies Drive Governance, Risk and Compliance Productivity Across Multiple Business Units 3
... , ... WX ), a,leading pharmaceutical, biotechnology, and medical-device research ... United States,announced that it had been selected to the ... consecutive year. The list recognizes the 50,fastest-growing companies in ...
... , DURHAM, N.C., Nov. 27 ... water-soluble carbon nanotube-based cancer drug delivery system in Hyderabad, India. ... walls that allows for conjugation with cancer drugs, was developed ... conjugated carbon nanotubes serves as a drug delivery vehicle with ...
... , BOSTON, Nov. 25 Biotech firm Replikins Ltd., ... pandemic through the prediction, outbreak, and progress of the current H1N1 ... new data shows that the lethality of the H1N1 ("Swine Flu") ... the virus,s current outbreak in the spring of 2009 to resting ...
Cached Biology Technology:WuXi PharmaTech Named in Deloitte Technology Fast 50 China 2009 List for the Fifth Consecutive Year 2North Carolina Based Cromoz Inc. Launches Carbon Nanotechnology for Target Drug Delivery System in Cancer Treatment in Hyderabad, India 2Biological H1N1 Vaccines: Too Little, Too Late 2Biological H1N1 Vaccines: Too Little, Too Late 3Biological H1N1 Vaccines: Too Little, Too Late 4Biological H1N1 Vaccines: Too Little, Too Late 5
(Date:2/5/2015)... , January 28, 2015 ... Analysis, Size And Segment Forecasts To 2020 has Been ... IR camera market is expected to reach USD 5.10 ... Grand View Research, Inc. IR cameras help identify the ... are expected to witness surging demand in medical imaging ...
(Date:2/5/2015)... 3, 2015 Despite a lack of ... such as consumer electronics, automotive, storage and telecommunication ... technology (SMT) screen printers. Innovations in electronics and ... push the adoption curve up. Meanwhile, sale volumes ... glue dispensers are indispensable in the production of ...
(Date:1/22/2015)... 13, 2015  Today, FreeWavz ( www.FreeWavz.com ), the ... campaign on Fundable, https://www.fundable.com/freewavz . FreeWavz will ... capacity to meet customer demand. Logo ... http://photos.prnewswire.com/prnh/20150113/168790 Invented by Dr. ...
Breaking Biology News(10 mins):IR Camera Market Worth $5.10 Billion By 2020: Grand View Research, Inc. 2IR Camera Market Worth $5.10 Billion By 2020: Grand View Research, Inc. 3End-user Demand Spurs Global SMT Screen Printers and Glue Dispensers Market 2End-user Demand Spurs Global SMT Screen Printers and Glue Dispensers Market 3FreeWavz Launches on Fundable to Drive Speed-to-Market in 2015 2
... A new book that delves into the unexpected properties ... Basic Books. "Life,s Ratchet: How Molecular Machines Extract Order from ... materials science and associate dean in Wayne State University,s College ... the science of levers and pulleys, atoms and quarks ...
... new study has found that the sensitivity and recovery of UK ... and degree of fragmentation of key habitat types in the landscape. ... Ecography , used data on the Ringlet butterfly collected from ... period which spanned a severe drought event in 1995. ...
... Nov. 1, 2012 TESARO, Inc. (Nasdaq: TSRO) announced ... Anaplastic Lymphoma Kinase (ALK) inhibitor, TSR-011, has commenced with ... G. Piper Cancer Center Clinical Trials at Scottsdale Healthcare, ... Research Institute (TGen). TSR-011 is an orally available ...
Cached Biology News:New book on physics principles by Wayne State professor explains life as we know it 2UK butterfly populations threatened by extreme drought and landscape fragmentation 2TESARO and Virginia G. Piper Cancer Center announce first patient in clinical trial of TSR-011 2
... Custom Peptide Services offer a full range ... research needs. Services include: Peptide synthesis, ... Peptide synthesis, purified product (70-95% pure) ... amidation, biotinylation, fluorescein, myristylation, phosphorylation, and rhodamine) ...
... is a new product number, ... product number. If showing no ... the old Sigma-Aldrich number (R4026) ... assistance. Material: cap polystyrene ...
... The GenePix Professional 4200A can be upgraded ... Autoloader automatically loads, scans, analyzes, and saves ... notifies you by email when the batch ... uniformity, even for warped slides. GenePix Pro ...
... Maximize the value of your microarray data ... support you need from consultation to extensive ... date methods. Utilize our expertise in combination ... from your own microarray experiments., ,Normalization and ...
Biology Products: