Navigation Links
Purification of Murine IgG1 Using UNOsphere S and CHT ,,, Ceramic Hydroxyapatite Chromatography, Rev A

Purification of Murine IgG1 Using UNOsphere S and CHT Ceramic Hydroxyapatite Chromatography, Rev A

Henry Lai, PhD, and Samuel G Franklin, PhD, Bio-Rad Laboratories, Inc., 2000 Alfred Nobel Drive, Hercules, CA 94547 USA


Introduction
Immunoglobulins (IgGs) from a variety of sources have been purified using the conventional process composed of a protein A chromatography step along with ion exchange chromatography and hydrophobic interaction chromatography steps (Jiskoot et al. 1989, Godfrey et al. 1993, Shadle et al. 1995, Ford et al. 2001). Protein A chromatography is normally applied as the first step in the process, yielding antibodies with very high purity in a single step. However, the disadvantages of protein A chromatography are: 1) antibody isomers are not well separated from different species; 2) protein A leakage requires additional chromatographic steps for protein A removal; and 3) protein A resins are substantially more expensive than ion exchange and ceramic hydroxyapatite supports.

Here we present an alternative process for antibody purification consisting of UNOsphere S chromatography and CHT ceramic hydroxyapatite chromatography. This process is simpler than the process utilizing protein A and avoids the leakage of a contaminating ligand (Jiskoot et al. 1989).

UNOsphere S support is a strong cation exchanger made from acrylamido and vinylic copolymers. It exhibits high protein binding capacity and low column backpressure at high linear velocity. Thus, it is a suitable capture resin in the downstream purification process. CHT ceramic hydroxyapatite support, with Ca2+ ions and PO4
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli
2. Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels
3. Yeast Protein Production System Features High Yields and One-Step Purification
4. Expression and Purification of Recombinant Proteins That Have Native Amino Acid Sequence
5. Easy Cosmid DNA Purification with StrataPrep Kits
6. MinElute PCR Purification Kit
7. QIAquick PCR Purification Kit
8. Microarray Target Purification Kit and the Microarray Target Synthesis Kit (T7)
9. Double-Affinity Purification of GST-His Tagged Proteins Using Vivapure 8-to-96 Well Cobalt-Chelate Kit
10. Ultra-Fast Purification of Small Nucleic Acids
11. RNAClean - RNA & cDNA in vitro Reaction Purification
Post Your Comments:
(Date:1/14/2014)... , Jan. 14, 2014 The largest international ... medicinal plants and therapeutic derivatives thereof has endorsed ... and researchers about the challenges of adulterated herb ... ) The Society for Medicinal ...
(Date:1/14/2014)... 2014 iLab Solutions, the leader in core ... Director of Product Strategy. In this role, Michelle will work ... to guide in the development of iLab products. Her main ... possible benefit to the scientific community by offering the most ...
(Date:1/14/2014)... WESTBOROUGH, Mass. , Jan. 14, 2014  RXi ... on discovering, developing and commercializing innovative therapies addressing ... announced that it received the Notice of Allowance ... on its unique self-delivering RNAi compounds (sd-rxRNA®), for ...
(Date:1/14/2014)... Kinaxis ®, provider of RapidResponse ®, a cloud-based supply ... ( S&OP ) service, is proud to be a gold ... held at the Hilton San Diego Bayfront, January 30 – ... Kaszas , Director of Supply Chain at Amgen, as she ...
Breaking Biology Technology:World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 2World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 3World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 4World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 5World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 6World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 7World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 8iLab Solutions Announces Michelle Detwiler as the New Director of Product Strategy 2RXi Pharmaceuticals Receives US Notice of Allowance for a Key Patent Relating to its Self-Delivering Technology with sd-rxRNAs targeting CTGF, including RXI-109, for the Treatment of Fibrotic Disorders 2RXi Pharmaceuticals Receives US Notice of Allowance for a Key Patent Relating to its Self-Delivering Technology with sd-rxRNAs targeting CTGF, including RXI-109, for the Treatment of Fibrotic Disorders 3Event Alert: Kinaxis Customer to Present at the Biomanufacturing Summit "Supply Chain: Improving Network Effectiveness" 2
... DIEGO, Jan. 3, 2012  Amylin Pharmaceuticals, Inc. (Nasdaq: ... Morgan Healthcare Conference in San Francisco on Monday, January 9, ... Daniel M. Bradbury, president and chief executive officer of ... presentation and break-out session will be webcast through the "Investors" ...
... Renowned biochemist Michael A. Marletta, PhD, assumed the post ... January 1, succeeding Richard A. Lerner, MD, who led ... (Photo:  http://photos.prnewswire.com/prnh/20120102/DC28698 ) "I,m excited ... knowledge in the biosciences and applying that knowledge to ...
... BioNeutral Group, Inc. (OTCBB: BONU), a specialty life ... received $400,000 in connection with the Preferred Stock ... ("Vinfluence"), New South Wales, Australia.  Pursuant to the ... initial closing and a second funding of $200,000, ...
Cached Biology Technology:Michael A. Marletta Takes Office as New President of Scripps Research Institute 2BioNeutral Group Receives $400,000 in Equity Financing 2BioNeutral Group Receives $400,000 in Equity Financing 3
(Date:4/23/2014)... who have had a stroke, often suffer motor deficits ... study conducted in Taiwan, that will be published in ... is currently freely available on-line as an unedited early ... one group of stroke victims had their own peripheral ... and a similar group did not, those who received ...
(Date:4/23/2014)... the energy landscape in the U.S., but in ... the energy industry, residents and agricultural interests over ... degraded water quality is a potential risk unless ... in the ACS journal Environmental Science & ... point out that a major criticism of extracting ...
(Date:4/23/2014)... Climate fiction, or simply cli-fi, is a newly coined term ... global warming. New research from University of Copenhagen shows how ... to simulate the potential consequences of climate change and imagine ... than scientific data on changes in the atmosphere; it is ... by the books we read and the films we see. ...
Breaking Biology News(10 mins):Autologous stem cell therapy improves motor function in chronic stroke victims 2How to avoid water wars between 'fracking' industry and residents 2Fiction prepares us for a world changed by global warming 2Fiction prepares us for a world changed by global warming 3
... as calcified, inert structures, but researchers at Columbia University ... important novel function of the skeleton. Theyve shown for ... organ that helps control our sugar metabolism and weight ... development of type 2 diabetes. The research, ...
... passed on from mother to child is decided very early ... ovary. In a cell division process that is unique to ... egg before it is fertilised. Using a powerful microscope, researchers ... how the molecular machinery functions that is responsible for chromosome ...
... Haven, Conn. It is not just whats in your genes, ... between species at least in yeast according to a ... Weve known for a while that the protein coding genes of ... author Michael Snyder , the Cullman Professor of Molecular ...
Cached Biology News:Research shows skeleton to be endocrine organ 2A unique arrangement for egg cell division 2Evolution is driven by gene regulation 2
This monoclonal antibody specifically recognizes Y. pestiscapsular F1 antigen. It does not cross-react with Y. pseudotuberculosisor Y. enterocolitica....
... was first identified as the cellular homolog of ... and Jun D have been shown to be ... which are involved in dimerization and DNA binding, ... transcriptional activation, diverge. Antigen: ...
IHC Zinc Fixative - , 1000 ml Consult technical datasheet for details....
... (IC) Fixation Buffer, used in conjunction ... 00-8333), can be used to fix ... intracellular staining of cytokines and other ... have been specially formulated for reducing ...
Biology Products: