Navigation Links
Purification of Insulin on UNO S1 Column, Rev A


Insulin is a peptide hormone which stimulates anabolic reactions for carbohydrates, proteins, and fats resulting in lowered blood glucose. It is used therapeutically for treatment of diabetes mellitus and was the first recombinant protein on the market. Although insulin is well characterized and understood, it is often difficult to purify because of the harsh pH and denaturing conditions needed to solubilize and disaggregate the protein. A comparison of the UNO S1 (Bio-Rad catalog # 720-0021) and a popular monodispersed cation exchange column showed that it was advantageous to use the UNO S1 column for small scale insulin separation or analysis.


Experimental
The insulin used in this study was an acid precipitate in suspension. The insulin was solubilized in acidic buffer with urea. The insulin sample was applied separately to an UNO S1 and a monodispersed cation exchange column and eluted by linear gradients. Fractions were collected and neutralized with ammonium hydroxide to pH 7.0. Due to the viscosity of the buffers, both columns were run at half the maximum recommended flow rates. The separation performed on the UNO S1 was run at 3 ml/min (Figure 1). The separation performed on the monodispersed cation exchanger was performed at 1 ml/min (Figure 2). Fractions 4350 and fractions 3034 were pooled from the separation performed on the UNO S1 and the monodispersed column respectively and analyzed by reversed phase HPLC. Analysis performed on Hi-Pore RP-304 reversed phase column (Bio-Rad catalog # 125-0551). The products from both columns were 98% pure. Figure 3 shows the HPLC chromatogram of the pooled fractions from the UNO S1 column after subtraction
'"/>

Source:


Page: All 1 2

Related biology technology :

1. High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli
2. Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels
3. Yeast Protein Production System Features High Yields and One-Step Purification
4. Expression and Purification of Recombinant Proteins That Have Native Amino Acid Sequence
5. Easy Cosmid DNA Purification with StrataPrep Kits
6. MinElute PCR Purification Kit
7. QIAquick PCR Purification Kit
8. Microarray Target Purification Kit and the Microarray Target Synthesis Kit (T7)
9. Double-Affinity Purification of GST-His Tagged Proteins Using Vivapure 8-to-96 Well Cobalt-Chelate Kit
10. Ultra-Fast Purification of Small Nucleic Acids
11. RNAClean - RNA & cDNA in vitro Reaction Purification
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Purification Insulin UNO Column Rev

(Date:10/2/2014)... MS Bioworks, a protein mass spectrometry ... its marketing approach. Backed by Promega — an industry ... the biological sciences, including industrial and academic clients. , ... growth over the past four years. To reach new ... plans to launch a monthly email newsletter, which will ...
(Date:10/2/2014)... 2014 The National Institutes of ... a grant today to develop an electrode array ... how the brain works through unprecedented resolution and ... NIH's efforts to support President Obama's BRAIN (Brain ... research effort to revolutionize our understanding of the ...
(Date:10/2/2014)... 2, 2014  CSSi ( www.CSSiEnroll.com ), the ... research industry, is pleased to announce its participation ... of October.  President Chris Trizna will ... in Clinical Trials New England conference sponsored by ... the annual Site Solutions Summit sponsored by the ...
(Date:10/2/2014)... SIRO Clinpharm, an award-winning Clinical ... prestigious Annual Conference of the Society for Clinical Data ... , where they were invited to speak at three ... analytics to support fraud detection, ,clinical data management,s role ... working together as virtual and integrated teams,. The award-winning ...
Breaking Biology Technology:Hile Creative to Provide Copywriting and Graphic Design Services for MS Bioworks 2NIH taps Lawrence Livermore to Develop Sophisticated Electrode Array System to Monitor Brain Activity 2NIH taps Lawrence Livermore to Develop Sophisticated Electrode Array System to Monitor Brain Activity 3NIH taps Lawrence Livermore to Develop Sophisticated Electrode Array System to Monitor Brain Activity 4Patient Recruitment Expert CSSi to be Featured at Upcoming Industry Events 2Patient Recruitment Expert CSSi to be Featured at Upcoming Industry Events 3SIRO Clinpharm Showcases Biometrics Capabilities at the SCDM 2014 Annual Conference in Las Vegas 2
... is,strengthening its customer-focused biomarker services portfolio to,pharmaceutical, ... Xie will lead those efforts as Battelle,s ... a suite of biomarker services, including biomarker,discovery, ... clients,better define molecular mechanisms of diseases, toxicity, ...
... against management - with six million, votes revoked ... change rules, TORONTO, June 25 /PRNewswire/ - The ... be in court today and will seek a,declaration that ... Meeting,was illegally held and will request that a proper ...
... probiotic ingredient, GanedenBC30, to power new raisin bran probiotic muffins. ... ... Main Street Gourmet, one of the fastest growing manufacturers of gourmet ... muffin fortified with the high-survivability probiotic, GanedenBC30 (Bacillus coagulans ...
Cached Biology Technology:Battelle Selects Xie to Develop Biomarker Services 2Main Street Gourmet Launches New Probiotic Muffins 2Main Street Gourmet Launches New Probiotic Muffins 3
(Date:9/30/2014)... The National Institutes of Health (NIH) awarded ... to develop an electrode array system that will ... works through unprecedented resolution and scale. , LLNL,s ... support President Obama,s BRAIN (Brain Research through ... effort to revolutionize our understanding of the human ...
(Date:9/30/2014)... reports positive results of a phase 1 clinical ... lung cancer marked by rearrangement of the gene ROS1. ... non-small cell lung cancer testing positive for ROS1 gene ... complete responses and 33 partial responses. Median progression-free survival ... resume its growth after being slowed by treatment ...
(Date:9/30/2014)... it comes to using marijuana, new research, involving mice ... Journal of Leukocyte Biology , suggests ... that you should. That,s because a team of Italian ... do serious long-term damage to the immune system. This ... diseases, such as multiple sclerosis, inflammatory bowel disease and ...
Breaking Biology News(10 mins):NIH taps lab to develop sophisticated electrode array system to monitor brain act 2NIH taps lab to develop sophisticated electrode array system to monitor brain act 3NEJM: Crizotinib effective in Phase 1 trial against ROS1 lung cancer 2Adolescent exposure to thc may cause immune systems to go up in smoke 2
... waves, known as gamma oscillations, for more than 50 ... memory. Now, for the first time, MIT researchers and ... by shining laser light directly onto the brains of ... developed technology known as optogenetics, which combines genetic engineering ...
... Aware, Inc. (Nasdaq: AWRE ), a ... announced the final results of its modified Dutch Auction ... City time, on Friday, April 17, 2009.In accordance with ... has accepted for purchase 3,500,252 shares of its common ...
... April 23, 2009 -- Deteriorating screws in bridges, fish that ... treat disease, the detected comeback of a long-gone whale, the ... teach us about cowardice. These are just a few ... meeting of the Acoustical Society of America (ASA), which convenes ...
Cached Biology News:MIT: Making waves in the brain 2Aware, Inc. Announces Final Results of Dutch Auction Tender Offer 2Aware, Inc. Announces Final Results of Dutch Auction Tender Offer 3Laughing hyenas, wailing levees, the sound of cheese and blaring bagpipes 2Laughing hyenas, wailing levees, the sound of cheese and blaring bagpipes 3Laughing hyenas, wailing levees, the sound of cheese and blaring bagpipes 4Laughing hyenas, wailing levees, the sound of cheese and blaring bagpipes 5Laughing hyenas, wailing levees, the sound of cheese and blaring bagpipes 6Laughing hyenas, wailing levees, the sound of cheese and blaring bagpipes 7Laughing hyenas, wailing levees, the sound of cheese and blaring bagpipes 8Laughing hyenas, wailing levees, the sound of cheese and blaring bagpipes 9Laughing hyenas, wailing levees, the sound of cheese and blaring bagpipes 10Laughing hyenas, wailing levees, the sound of cheese and blaring bagpipes 11Laughing hyenas, wailing levees, the sound of cheese and blaring bagpipes 12Laughing hyenas, wailing levees, the sound of cheese and blaring bagpipes 13Laughing hyenas, wailing levees, the sound of cheese and blaring bagpipes 14Laughing hyenas, wailing levees, the sound of cheese and blaring bagpipes 15
Bethyl Laboratories packages antibodies, conjugates and calibrators to provide quantitative ELISA kits. Each kit contains the following components, sufficient for 1000 single well assays....
Bethyl Laboratories packages antibodies, conjugates and calibrators to provide quantitative ELISA kits. Each kit contains the following components, sufficient for 1000 single well assays....
Bethyl Laboratories packages antibodies, conjugates and calibrators to provide quantitative ELISA kits. Each kit contains the following components, sufficient for 1000 single well assays....
Use our experts and resources to develop reagent systems for your applications....
Biology Products: