Navigation Links
Pseudomonas putida

Multiporator / Electroporator 2510 Transformation Protocol Protocol No. 4308 915.529 01/2002 Microorganism Pseudomonas putida P8 Cell type Bacteria, gram negative Molecules injected Plasmid DNA Growth medium Standard 1-medium Washing solution 10% glycerol Electroporation solution 10% glycerol Outgrowth medium Standard 1-medium Cuvette 2 mm gap width Reference Prof. Friedhelm Meinhardt Universitt Mnster Corrensstrae 3 D-48149 Mnster
e-mail: meinhar@uni-muenster.de Making electrocompetent cells:

1. Inoculate 50 ml standard-1 medium with 7 ml of a fresh overnight culture of Pseudomonas putida. Grow cells at 30 C to a density of O.D. of 0.8. 2. Harvest by centrifugation. 3. Wash twice with 50 ml ice-cold glycerol, centrifuge. 4. Resuspend cells in 0.8 ml ice-cold glycerol, keep on ice.

Electroporation of cells:

  1. Add 4 l plasmid DNA (1 g) to 40 l of electrocompetent cells. Homogenize by gently mixing with pipette several
    times. Transfer mixture into a prechilled cuvette.
  2. Wipe moisture from the cuvette and insert the cuvette into the device.
  3. Electroporation:

    Mode Prokaryotes O Voltage (V) 2,400 V Time constant (T) 5 ms
  4. Immediately add 1 ml standard 1-medium. Incubate 2 hours at 30 C.
  5. Plate cells on selective plates.
Expected Results: Transformation efficiency up to 2 x 104 transformants/g of DNA.


'"/>

Source:


Page: All 1 2

Related biology technology :

1. Pseudomonas aeruginosa
2. Pseudomonas putida
3. Pseudomonas aeruginosa
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:2/12/2016)... 2016  PTC Therapeutics, Inc. (NASDAQ: PTCT ... to Realize Innovation, Vision and Empowerment) grant award ... funds to patient advocacy organizations to develop unique ... to the rare disease community by increasing awareness, ... advocates. Mary Frances Harmon , ...
(Date:2/11/2016)... ORLANDO, Florida , February 11, 2016 ... Populations and Promote Genetic Understanding to Support Research and Discovery ... consortium, GenomeAsia 100K, today announced an ambitious plan to sequence ... 12 South Asian countries and at least 7 of North ... --> In the first phase, the project will focus ...
(Date:2/11/2016)... , Feb. 11, 2016  Vermillion, Inc. (NASDAQ: ... gynecologic disease, today announced the formation of the Steering ... --> --> Pelvic masses can ... diagnosis and management. Once pregnancy is ruled out, pelvic ... and ovaries, advanced endometriosis, benign ovarian tumors and gastrointestinal ...
(Date:2/11/2016)... NEW YORK , Feb. 11, 2016  Bioethics International, ... how medicines are researched, developed, marketed and made accessible to ... BMJ Open had named the publication of the ... for 2015. The publication is also featured as one of ... published in the last year that are most frequently read. ...
Breaking Biology Technology:
(Date:2/3/2016)... , Feb. 3, 2016 ... the addition of the "Emotion Detection ... Machine Learning, and Others), Software Tools (Facial ... Areas, End Users,and Regions - Global forecast ... --> http://www.researchandmarkets.com/research/d8zjcd/emotion_detection ) has ...
(Date:2/2/2016)... Va. , Feb. 2, 2016   ... award from the U.S. Army Research Office and ... the range and sensitivity of the company,s ... Past Accounting Mission and, more generally, defense-related DNA ... DNA phenotyping capabilities (predicting appearance and ancestry from ...
(Date:1/28/2016)... 2016 Synaptics (NASDAQ: SYNA ), a leading developer ... quarter ended December 31, 2015. --> ... 2016 increased 2 percent compared to the comparable quarter last year ... 2016 was $35.0 million, or $0.93 per diluted share. ... the first quarter of fiscal 2016 grew 9 percent over the ...
Breaking Biology News(10 mins):