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Pseudomonas putida

  • Add 4 l plasmid DNA (1 g) to 40 l of electrocompetent cells. Homogenize by gently mixing with pipette several
    times. Transfer mixture into a prechilled cuvette.
  • Wipe moisture from the cuvette and insert the cuvette into the device.
  • Electroporation:

    Mode Prokaryotes O Voltage (V) 2,400 V Time constant (T) 5 ms
  • Immediately add 1 ml standard 1-medium. Incubate 2 hours at 30 C.
  • Plate cells on selective plates.
  • Expected Results: Transformation efficiency up to 2 x 104 transformants/g of DNA.


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