Navigation Links
Pseudomonas aeruginosa

Multiporator / Electroporator 2510 Transformation Protocol Protocol No. 4308 915.528 01/2002 Microorganism Pseudomonas aeruginosa Cell type Bacteria, gram negative Molecules injected Plasmid DNA (pUC 18 with 1.8 kb insert in water) Growth medium LB medium Washing solution 300 mM sucrose Electroporation solution 300 mM sucrose Outgrowth medium LB medium Cuvette 2 mm gap width Reference Smith A.W. and Iglewski B.H. 1989 Nucleic Acids Research 17, No. 24 10509 Making electrocompetent cells:

1. Grow cells in LB medium at 37 C with shaking up to an O.D.540 of 0.3-05. 2. Harvest by centrifugation (7,000 x g, 10 min, at 4 C). 3. Wash pellet in the original volume with sucrose, centrifuge and wash again in _ volume of washing solution. 4. Resuspend in 300 mM sucrose to a final concentration of 1011 cells/ml, chill on ice for 30 minutes.

Electr
'"/>

Source:


Page: All 1 2

Related biology technology :

1. Pseudomonas aeruginosa
2. Pseudomonas putida
3. Pseudomonas putida
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Pseudomonas aeruginosa

(Date:8/1/2014)... (PRWEB) August 01, 2014 RURO, ... Solutions provider, today officially announces the opening of ... Boji Science Park, Baoshan District in Shanghai, PRC, ... is under the management of Mr. Shen Gang, ... In the past three years, RURO’s has installed ...
(Date:8/1/2014)... York , August 1, 2014 ... Transparency Market Research "Biophotonics Market - Global Industry Analysis, ... the market was valued at USD 25.16 billion in ... by 2020, growing at a CAGR of 10.4% from ... Market Report at  http://www.transparencymarketresearch.com/biophotonics-market.html Increasing demand ...
(Date:8/1/2014)... , Aug. 1, 2014 Glencoe ... launch of "Data InPress" ( http://glencoesoftware.com/data-inpress.html ), ... integrating multidimensional image data into on-line publications.  ... and Video Injection Service. Photo ... http://photos.prnewswire.com/prnh/20140731/132409 The ...
(Date:7/31/2014)... Nearly 20,000 medical professionals and healthcare leaders ... Clinical Lab Expo in Chicago ... diagnostic research and technology that will advance medicine and ... of Wednesday, July 30, more than 19,500 attendees had ... Lab Expo, with more than 10,000 of these attendees ...
Breaking Biology Technology:RURO Opens Office in Shanghai, China 2Global Biophotonics Market is Expected to Reach USD 50.20 Billion in 2020: Transparency Market Research 2Global Biophotonics Market is Expected to Reach USD 50.20 Billion in 2020: Transparency Market Research 3Global Biophotonics Market is Expected to Reach USD 50.20 Billion in 2020: Transparency Market Research 4Glencoe Software Launches Data InPress 2Glencoe Software Launches Data InPress 3Research on Digital Health, Alzheimer's, Ebola Draws Nearly 20,000 Attendees to 2014 AACC Annual Meeting & Clinical Lab Expo 2Research on Digital Health, Alzheimer's, Ebola Draws Nearly 20,000 Attendees to 2014 AACC Annual Meeting & Clinical Lab Expo 3
... March 4 Yesterday Ventria,Bioscience received the ... County,School District Board. Ventria received the award ... in biotechnology for students,and teachers. Ventria has ... internships in biotechnology. In 2007, Ventria provided ...
... Integration of Cardium Health, AirLogix, and Work/Life Innovations into ... ... Company, BEDFORD, Mass., March 4 CaseNET, Inc., ... a subsidiary of Centene Corporation (NYSE: CNC ), has selected,the ...
... Continues Progress at Seneca, Illinois ... Biodiesel Refinery, ... ) announced today that it has satisfied the conditions precedent,and completed ... credit facility with WestLB AG, one of the world,s leading,financial services ...
Cached Biology Technology:Ventria Receives Friends of Education Award From Geary County School District 2Nurtur(TM) Selects CaseNET to Enable Integrated Health and Life Management Capabilities 2Nova Biosource Fuels Announces First Funding Under $41 Million Senior Secured Credit Facility 2
(Date:7/31/2014)... hidden hazard lurks beneath many of the roughly ... The hazard is corrosion in parts of underground ... leaks and contamination of groundwater, a source of ... nine states have reported many rapidly corroding gas ... incidents are generally associated with use of gasoline-ethanol ...
(Date:7/31/2014)... 2014 -- Do people make a rational choice to ... that way? Is it a matter of genetics?, Two ... colleague from Rice University say that neither conscious decision-making ... left while others lean right. , A growing body ... are at the core of political differences, the researchers ...
(Date:7/31/2014)... was in full fury, raging swiftly from crown to ... of Yosemite National Park in California,s Sierra Nevada in ... began to turn, enacting a case study in the ... large, severe fires. , "When the Rim Fire hit ... been used as a management tool, rather than immediately ...
Breaking Biology News(10 mins):NIST corrosion lab tests suggest need for underground gas tank retrofits 2Scientists find growing consensus: Political attitudes derive from body and mind 2The Rim Fire 1 year later: A natural experiment in fire ecology and management 2The Rim Fire 1 year later: A natural experiment in fire ecology and management 3The Rim Fire 1 year later: A natural experiment in fire ecology and management 4The Rim Fire 1 year later: A natural experiment in fire ecology and management 5
... headed by Prof. Johanna Ivaska (University of Turku and VTT ... of Turku and the National Institute for Health and Welfare) ... Published in a leading journal, the study concludes ... and of lung and prostate cancer cells. It is likely ...
... scientists have developed a new, integrated, ten-year science plan to ... role in it. Understanding the carbon cycle is central for ... builds on the first such plan, published in 1999, but ... as agents and managers of carbon cycling and climate change, ...
... COPENHAGEN (16 November 2011) In the lead up ... this month, an independent global commission of eminent scientists today ... how to achieve food security in the face of climate ... commissioners urged immediate, coordinated action toward transforming the food system ...
Cached Biology News:Scientists tackle the carbon conundrum 2Global commission charts pathway for achieving food security in face of climate change 2Global commission charts pathway for achieving food security in face of climate change 3Global commission charts pathway for achieving food security in face of climate change 4Global commission charts pathway for achieving food security in face of climate change 5Global commission charts pathway for achieving food security in face of climate change 6Global commission charts pathway for achieving food security in face of climate change 7
I-Ab MHC Class II...
... mm Culture Dishes BD BioCoat Collagen I ... a uniform application of rat tail collagen type ... ,Surface : ECM/attachment factors ,Surface ... : 60 mm x 15 mm ...
The piston kit includes 2 pistons to service one F10 pump of a BioLogic DuoFlow workstation....
BD BioCoat BD MatrigelTM Matrix 100 mm Culture Dishes for hepatocytes, tissue-culture treated polystyrene with a uniform application of MATRIGEL Matrix....
Biology Products: