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Protocol for GenomePlex Whole Genome Amplification from Soil

e Elution Solution diluted 10-fold with water.

  • To elute the DNA, centrifuge the column at maximum speed for 1 minute. The PCR amplification product is now present in the eluate and is ready for immediate use or storage at -20 C.

    Appendix

    References
    1. Barker, D. L., et al. Two methods of whole-genome amplification enable accurate genotyping across a 2320-SNP linkage panel. Genomic Research, 14, 901-7 (2004).

    2. Gribble, S., et al. Chromosome paints from single copies of chromosomes. Chromosome Research, 12, 143-51 (2004).

    3. Thorstenson, Y. R., et al. An Automated Hydrodynamic Process for Controlled, Unbiased DNA Shearing. Genome Research, 8, 848-855 (1998).

    Contact Information
    For technical assistance please contact:
    Technical Service
    (800) 325-5832
    www.techserv@sial.com

    To learn more about GenomePlex WGA technology visit: www.sigmaaldrich.com

    GenomePlex is a registered trademark of Rubicon Genomics, Inc.
    GenomePlex WGA technology patent pending.
    GenElute and Jumpstart are trademarks of Sigma-Aldrich, Inc.
    Nanodrop is a registered trademark of Nanodrop Technologies, Inc.
    PicoGreen is a trademark of Molecular Probes, Inc.
    UltraClean is a trademark of Mo Bio Laboratories, Inc.
    BeadArray is a Trademark of Illumina, Inc.
    TaqMan is a registered trademark of Roche Molecular Systems
    Taq antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.
    This product is sold under license from Roche Molecular Systems, Inc. and Applied Biosystems.
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