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Protocol for GenomePlex Whole Genome Amplification from Soil

Application Guide

I. Description
II. Product Components
III. Materials to be Supplied by the User
IV. Protocol for Extraction of DNA from Soil
V. Protocol for GenomePlex Whole Genome Amplification from Soil
VI. Quantification of Amplified products
VII. Purification of Amplified Products
Appendix
References
Contact Information

Application Guide

I. Description
This protocol addresses the need for extracting, amplifying, and purifying genomic DNA from soil samples. Genomic DNA from the soil samples can be damaged and of low yield. These qualities hurt the researchers ability to perform downstream analysis. GenomePlex WGA provides a method of amplifying nanogram quantities of genomic DNA from soil with little or no detectable bias. The methods described use a soil extraction kit, GenomePlex WGA kit, and GenElute PCR Clean-up Kit.

This technology maintains the genetic representation with concordance >99.8% in genotyping results from comparing genomic DNA and GenomePlex amplified DNA. In addition to SNP genotyping, downstream applications also include performing TaqMan assays and BeadArray analysis.

II. Product Components

  • UltraClean Soil Kit by Mo Bio Laboratories, Inc. (12800-50)
  • GenomePlex Whole Genome Amplification Kit (WGA1)
  • GenElute PCR Clean-Up Kit (NA1020)

III. Materials to be Supplied by the User

  • Soil samples
  • 1.5 ml microcentrifuge tubes for lysis, Product Code T9661
  • Microcentrifuge (with rotor for 2ml tubes)
  • Ethanol (absolute), Product Code E7023
  • Molecular Biology
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