Navigation Links
Protocol for GenomePlex Whole Genome Amplification from Plant

Application Guide

I. Description
II. Product Components
III. Materials to be Supplied by the User
IV. Protocol for Extraction of DNA from Plant
V. Protocol for GenomePlex Whole Genome Amplification from Plant
VI. Quantification of Amplified Products
VII. Purification of Amplified Products
Appendix
References
Contact Information

Application Guide

I. Description
Extracting DNA from plant tissue is a complicated process due to the tough cell wall that surrounds most plant cells. Genomic DNA from plant material can be damaged and low yield. These qualities challenge the researchers ability to perform downstream analysis. GenomePlex WGA provides a method of amplifying nanogram quantities of genomic DNA from plant with little or no detectable bias. The methods described use the GenElute Plant Genomic DNA Miniprep Kit, GenomePlex WGA kit, and GenElute PCR Clean-up Kit.

GenomePlex is a Whole Genome Amplification (WGA) method that allows the researcher to generate a representative, approximate 1000-fold amplification of genomic DNA. The kit utilizes a proprietary amplification technology based upon random fragmentation of genomic DNA and conversion of the resulting small fragments to PCR-amplifiable OmniPlex Library molecules flanked by universal priming sites. The OmniPlex library is then PCR amplified using universal oligonucleotide primers and a limited number of cycles. This technology maintains the genetic representation with concordance >99.8% in genotyping results from comparing genomic DNA and GenomePlex amplified DNA. In addition to SNP genotyping, downstream applications also include performing TaqMan assays and
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. New Protocols for Isolating High- Molecular-Weight Genomic DNA
2. Eppendorf Multiporator Transfection Protocols for Eukaryotic Cells
3. Lambda DNA Extraction Protocol
4. M13/Phagemid DNA Extraction Protocol
5. Mouse Tail Genomic DNA Isolation Protocol(1)
6. Genomic DNA Isolation Protocol(1,2,3)
7. Basic Plasmid DNA Isolation Protocol
8. Protocol for RNA Isolation Using TRIzol Reagent with Phase Lock Gel-Heavy
9. RNA-free Plasmid DNA Isolation Protocol
10. Lambda DNA Extraction Protocol
11. Eppendorf Multiporator Transfection Protocols for Eukaryotic Cells
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Protocol for GenomePlex Whole Genome Amplification from Plant

(Date:7/31/2015)... ... July 31, 2015 , ... ... analysis and premium industry insights on the global resorcinol industry. The report highlights ... study of vivid market scenarios and analysis of primary and secondary inputs from ...
(Date:7/31/2015)... ... July 31, 2015 , ... ... and quantification of partially hydrolyzed gluten in foods, has been accepted by AOAC ... method “Partially Hydrolyzed Gluten in Fermented Cereal-Based Products by R5 Competitive ELISA,” based ...
(Date:7/30/2015)... -- AACC welcomed thousands of medical professionals and ... & Clinical Lab Expo in Atlanta ... revolutionary advancements in clinical testing technology and research ... diagnose patients quickly and accurately and make sure ... As of Wednesday, July 29, more than 17,500 ...
(Date:7/30/2015)... ... July 30, 2015 , ... ... Vieillard as Sales & Marketing Director. , With more than 25 years ... responsible for reinforcing Tronics’ business development activities worldwide. He brings to the company ...
Breaking Biology Technology:Resorcinol Market to Grow at 6% CAGR to 2019 Says New Research Report at ReportsnReports.com 2Resorcinol Market to Grow at 6% CAGR to 2019 Says New Research Report at ReportsnReports.com 3Resorcinol Market to Grow at 6% CAGR to 2019 Says New Research Report at ReportsnReports.com 4RIDASCREEN® Competitve Gliadin Receives AOAC Official First Action Status 2Innovative Research on Cancer, Heart Disease, and Infectious Diseases Draws More than 17,500 Attendees to 2015 AACC Annual Meeting 2Innovative Research on Cancer, Heart Disease, and Infectious Diseases Draws More than 17,500 Attendees to 2015 AACC Annual Meeting 3Innovative Research on Cancer, Heart Disease, and Infectious Diseases Draws More than 17,500 Attendees to 2015 AACC Annual Meeting 4Tronics appoints Francois Vieillard as Sales and Marketing Director 2
... ... ... Inc., a stem cell,engineering company, today announced data demonstrating for the first time,that ... insulin in mice. The findings are reported in an,article appearing on-line today, in ...
... net revenues grew 29.8%, as compared to Q4 2006, to RMB45.7 ... - Q4 2007 net income grew 152.2%, as compared to Q4 2006, to RMB17.9 ... (US$2.5 million), - 2007 fiscal year net revenues increased 41.0% year-over-year to RMB180.2 ... 2007 fiscal year net income grew 167.4% year-over-year to RMB81.5 million ...
... 20 Addrenex,Pharmaceuticals has reached its first milestone ... drug application (NDA) for its first drug,CloniBID, to ... Drug,Administration, Addrenex and its licensing partner Sciele Pharma, ... of 2009., Addrenex has moved swiftly to ...
Cached Biology Technology:Novocell Reports Successful Use of Stem Cells to Generate Insulin in Mice 2Novocell Reports Successful Use of Stem Cells to Generate Insulin in Mice 33SBio Inc. Announces Unaudited Fourth Quarter and Fiscal Year 2007 Results 23SBio Inc. Announces Unaudited Fourth Quarter and Fiscal Year 2007 Results 33SBio Inc. Announces Unaudited Fourth Quarter and Fiscal Year 2007 Results 43SBio Inc. Announces Unaudited Fourth Quarter and Fiscal Year 2007 Results 53SBio Inc. Announces Unaudited Fourth Quarter and Fiscal Year 2007 Results 63SBio Inc. Announces Unaudited Fourth Quarter and Fiscal Year 2007 Results 73SBio Inc. Announces Unaudited Fourth Quarter and Fiscal Year 2007 Results 83SBio Inc. Announces Unaudited Fourth Quarter and Fiscal Year 2007 Results 93SBio Inc. Announces Unaudited Fourth Quarter and Fiscal Year 2007 Results 103SBio Inc. Announces Unaudited Fourth Quarter and Fiscal Year 2007 Results 113SBio Inc. Announces Unaudited Fourth Quarter and Fiscal Year 2007 Results 123SBio Inc. Announces Unaudited Fourth Quarter and Fiscal Year 2007 Results 133SBio Inc. Announces Unaudited Fourth Quarter and Fiscal Year 2007 Results 143SBio Inc. Announces Unaudited Fourth Quarter and Fiscal Year 2007 Results 153SBio Inc. Announces Unaudited Fourth Quarter and Fiscal Year 2007 Results 16Addrenex Pharmaceuticals Reaches Milestone with Filing of NDA for First Product, CloniBID, to Treat Hypertension 2
(Date:7/8/2015)... , July 8, 2015 Summary Pancreatic ... the fourth most fatal, with a mortality rate of ... prognosis of pancreatic cancer patients has highlighted a significant ... is not being met by the current market. ... of products with varying molecule types and mechanisms of ...
(Date:7/8/2015)... 8, 2015  Trovagene, Inc. (NASDAQ: TROV ... announced the launch of a study that aims ... Monitoring℠ (PCM) technology for predicting response to treatment ... combination of the novel immunotherapy agents Yervoy® (ipilumumab), ... inhibitor. The 50-patient study will be led by ...
(Date:7/7/2015)... , June 30, 2015 ... announced the addition of the "Capacitive Fingerprint ... To this date, fingerprint sensing technology ... and fingerprint sensors are well developed. This patent ... technology. The domain of capacitive fingerprint ...
Breaking Biology News(10 mins):Frontier Pharma: Pancreatic Cancer - Identifying and Commercializing First-in-Class Innovation 2Frontier Pharma: Pancreatic Cancer - Identifying and Commercializing First-in-Class Innovation 3Trovagene Initiates Study to Monitor Response to Immunotherapy in Melanoma Patients Using its Precision Cancer Monitoring Platform 2Trovagene Initiates Study to Monitor Response to Immunotherapy in Melanoma Patients Using its Precision Cancer Monitoring Platform 3Trovagene Initiates Study to Monitor Response to Immunotherapy in Melanoma Patients Using its Precision Cancer Monitoring Platform 4Worldwide Capacitive Fingerprint Sensors Patent Landscape Market Report 2015-2020 - Main IP Holders include Seiko Epson, AuthenTec, Sony and STMicroelectronics 2
... meeting next week may decide the fate of tuna in ... marine resources. The Inter-American Tropical Tuna Commission (IATTC) ... 27 in Panama City, Panama, to reverse a trend of ... tuna stocks. Failure of the IATTC to compromise effectively in ...
... N.Y., June 17 bioMETRX, Inc. (OTC,Bulletin Board: ... today announced that the Handgun Club of America ... are showing great interest in,bioMETRX,s smartTOUCH(TM) bioVAULT 2.0 ... with the touch of a finger, and recognition,taking ...
... latest state of the environment report, released today, ... issues facing the NAFTA partners. The report ... and ecosystems, and pollution. Specific topics include climate ... porpoiseand the quality and quantity of water shared ...
Cached Biology News:Tuna populations at risk 2bioMETRX, Inc. Gun Storage Products Endorsed by the Handgun Club of America 2bioMETRX, Inc. Gun Storage Products Endorsed by the Handgun Club of America 3New report looks at the state of the North American environment 2
Incomplete Adjuvant...
... Lacks the mycobacteria found ... side-effects. For this reason, Incomplete, ... Freund's Adjuvants may be used to produce water-in-oil ... emulsions stimulate high and long-lasting antibody responses which ...
2,4-Dinitrophenyl hapten is conjugated to Human Serum Albumin (HSA) protein through lysine....
HiTrap Streptavidin HP, 5 x 1 ml. Category: Chromatography Systems & Accessories, Systems....
Biology Products: