Navigation Links
Protocol for GenomePlex Whole Genome Amplification from Plant

Application Guide

I. Description
II. Product Components
III. Materials to be Supplied by the User
IV. Protocol for Extraction of DNA from Plant
V. Protocol for GenomePlex Whole Genome Amplification from Plant
VI. Quantification of Amplified Products
VII. Purification of Amplified Products
Appendix
References
Contact Information

Application Guide

I. Description
Extracting DNA from plant tissue is a complicated process due to the tough cell wall that surrounds most plant cells. Genomic DNA from plant material can be damaged and low yield. These qualities challenge the researchers ability to perform downstream analysis. GenomePlex WGA provides a method of amplifying nanogram quantities of genomic DNA from plant with little or no detectable bias. The methods described use the GenElute Plant Genomic DNA Miniprep Kit, GenomePlex WGA kit, and GenElute PCR Clean-up Kit.

GenomePlex is a Whole Genome Amplification (WGA) method that allows the researcher to generate a representative, approximate 1000-fold amplification of genomic DNA. The kit utilizes a proprietary amplification technology based upon random fragmentation of genomic DNA and conversion of the resulting small fragments to PCR-amplifiable OmniPlex Library molecules flanked by universal priming sites. The OmniPlex library is then PCR amplified using universal oligonucleotide primers and a limited number of cycles. This technology maintains the genetic representation with concordance >99.8% in genotyping results from comparing genomic DNA and GenomePlex amplified DNA. In addition to SNP genotyping, downstream applications also include performing TaqMan assays and
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. New Protocols for Isolating High- Molecular-Weight Genomic DNA
2. Eppendorf Multiporator Transfection Protocols for Eukaryotic Cells
3. Lambda DNA Extraction Protocol
4. M13/Phagemid DNA Extraction Protocol
5. Mouse Tail Genomic DNA Isolation Protocol(1)
6. Genomic DNA Isolation Protocol(1,2,3)
7. Basic Plasmid DNA Isolation Protocol
8. Protocol for RNA Isolation Using TRIzol Reagent with Phase Lock Gel-Heavy
9. RNA-free Plasmid DNA Isolation Protocol
10. Lambda DNA Extraction Protocol
11. Eppendorf Multiporator Transfection Protocols for Eukaryotic Cells
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Protocol for GenomePlex Whole Genome Amplification from Plant

(Date:5/1/2015)... 2015 /CNW/ - Softchoice, a leading North American IT solutions and ... recipient of two Cisco® Partner Summit Country awards. ... and Security Partner of the Year for Canada ... week in Montreal . ... partner awards at this year,s conference," said David MacDonald ...
(Date:5/1/2015)... -- According to a new market ... by Type (Monoclonal, Polyclonal), by Technology (Hybridoma), by ... Analysis & Global Forecast to 2019", published by ... Market for the forecast period of 2014 to ... Billion by 2019 from $1.425 Billion in 2014, ...
(Date:4/30/2015)... , April 30, 2015   Tamir Biotechnology , ... an update on its Ebola antiviral therapy program. ... candidates, TMR004, is currently being evaluated and considered by ... battle against Ebola. During the first quarter of 2015, ... Meeting To Accelerate The Evaluation of Potential Treatments and ...
(Date:4/30/2015)... 30, 2015 Oxford Finance LLC ("Oxford"), a ... sciences and healthcare services companies, today announced the closing ... with Celula, Inc., a diagnostic testing company focused on ... patient care and improved outcomes.  Proceeds of the loan ... the commercialization of its products in the U.S. and ...
Breaking Biology Technology:Softchoice recognized with two Country Awards at Cisco Partner Summit 2015 2Antibody Production Market worth $2.572 Billion by 2019 2Antibody Production Market worth $2.572 Billion by 2019 3Antibody Production Market worth $2.572 Billion by 2019 4Tamir Biotechnology Provides Update on TMR004 Development Against Mutating Ebola 2Tamir Biotechnology Provides Update on TMR004 Development Against Mutating Ebola 3Tamir Biotechnology Provides Update on TMR004 Development Against Mutating Ebola 4Oxford Finance Provides $10 Million Debt Financing to Celula 2
... Sept. 12 Millennium,Biotechnologies Group, Inc. (OTC ... Mirken has been elected President and Chief ... Biotechnologies, Inc. Mr. Mirken,brings more that 18 ... and marketing expertise to Millennium. Mr. Mirken ...
... ), a pharmaceutical company focused on the discovery and,development of ... and,cancer, announced today that Tom Chesterman, SVP and CFO of ... Conference in New,York. The presentation is scheduled to begin ... New York Palace Hotel in New York. A live ...
... National One-Day Meetings Scheduled for London, Paris, Munich, San ... ... 12 Thomson Scientific,part of The Thomson Corporation (NYSE: ... solutions to the worldwide research and business,communities, announced today ...
Cached Biology Technology:Millennium Biotechnologies Group, Inc. (MBTG.OB) Announces Appointment of Mark C. Mirken as its President and Chief Operating Officer 2Millennium Biotechnologies Group, Inc. (MBTG.OB) Announces Appointment of Mark C. Mirken as its President and Chief Operating Officer 3Bionovo to Present at A.G. Edwards' 2nd Annual Emerging Growth Conference 2007 2Thomson Scientific to Host Pharmaceutical Vision - A User's Conference for Information, Regulatory Affairs, Competitive Intelligence and Biotechnology Professionals 2
(Date:4/2/2015)... According to preliminary estimates Fingerprint ... higher than 125 MSEK. This exceeds the guidance provided ... revenues for Q1 2015 would be in line with ... 2014. The operating result for the first quarter is ... report will as previously communicated be published on May ...
(Date:3/31/2015)... YORK , March 31, 2015   Guidepoint ... the launch of the Post-Surgical Pain Management TRACKER, part ... products tracking usage trends in the medical device and ... access to data including treatment volumes, market share, and ... the pain associated with joint surgery. The ...
(Date:3/24/2015)... 24, 2015   NexID Biometrics LLC, whose ... needs, today announced the beginning of shipments of version ... The company, based in Potsdam, N.Y. ... Connect:ID Expo, which began here today at the Walter ... 2.0 of its SDK boosts the accuracy rate range ...
Breaking Biology News(10 mins):Fingerprint Cards Revises Revenues for Q1 2015 2Guidepoint Launches Post-Surgical Pain Management TRACKER 2Guidepoint Launches Post-Surgical Pain Management TRACKER 3NexID Begins Shipments Of Version 2.0 Fake-Finger-Detection Solution 2
... Ottawa, Ontario (April 30, 2012) In a rapid ... Aquatic Sciences, biologist Douglas Swain of the Gulf Fisheries Centre ... Oceanography present findings that suggest the delay in recovery of ... to increased predation by grey seals or other governing factors ...
... IPM in Tehran and the University of Cambridge, UK, and ... frozen electric charges can make a difference when they are ... a twisting force that is strong enough to be felt ... about to be published in EPJ E (1), could help ...
... FASEB Journal suggests that a specific ... death induced by microgravity environments, and that inhibiting this ... of immune problems in astronauts caused by spaceflight. ... the aging process on Earth, this discovery may also ...
Cached Biology News:Scientists discover enzyme that could slow part of the aging process in astronauts -- and the elderly 2
... of robotic workstations is designed for laboratories ... the ideal automation platform for routine sample ... procedures, improving throughput, process consistency and safety. ... software allows you to control the robotic ...
... utilizes a silica based spin filter membrane to ... desired DNA band is cut from an agarose ... unit. The gel slice is melted irreversibly in ... is bound to the silica spin filter membrane ...
... to HDAC4 - ChIP Grade HDAC4 ... mammalian histone deacetylases, which consists of 1084 ... highly similar to the deacetylase domain of ... between the nucleus and cytoplasm in a ...
... pACHLT-A-BFP Baculovirus Transfer Vector is a derivative of ... gene for blue fluorescent protein (BFP), followed by ... (MCS).,Foreign genes are expressed as BFP-6xHis fusion proteins ... enzyme,sites (EcoR I, Stu I, Sac I, Not ...
Biology Products: