Navigation Links
Protocol for GenomePlex Whole Genome Amplification from Blood Card

tide primers and a limited number of cycles. This technology maintains the genetic representation with concordance >99.8% in genotyping results from comparing genomic DNA and GenomePlex amplified DNA1. In addition to SNP genotyping, downstream applications also include performing TaqMan assays and BeadArray analysis.

II. Product Components

  • GenElute Blood Genomic DNA Kit (NA2000)
  • GenomePlex Whole Genome Amplification Kit (WGA1)
  • GenElute PCR Clean-Up Kit (NA1020)
III. Materials to be Supplied by the User
  • Blood Card
  • 1.5 ml microcentrifuge tubes for lysis
  • Microcentrifuge (with rotor for 2ml tubes)
  • Ethanol (absolute) Product Code E7023
  • Molecular Biology Reagent Water, Product Code W4502
  • JumpStart Taq DNA Polymerase, Product Code D9307
  • 55 C water bath or heating block
IV. Protocol for Extraction of DNA from Blood Card
  • Performed with GenElute Blood Genomic DNA (NA2000)
    1. Cut a disc from a dried blood card (200 l spotted) into several 2 mm by 2 mm pieces and place the pieces into a 1.5 ml microcentrifuge tube.
    2. Add 40 l Proteinase K and 1.0 ml Resuspension Solution.
    3. Add 300 l of Lysis Solution C and vortex thoroughly for 15 seconds.
    4. Incubate at 55 C for 10 minutes.
    5. After the incubation, transfer the liquid (discard blood card remaining in the microcentrifuge tube) to a 15ml conical tube.
    6. Add 500 l of Column Preparation Solution to the GenElute Miniprep Binding Column (red o-ring) and centrifuge at 12,000 x g for 1 minute. Discard the flow-through liquid.
    7. Add 900 l of 95-100% ethanol to the lysate in the 15 ml conical tube and mix thoroughly by vortexing 5 to 10 seconds.
    8. Transfer the contents of the tube into the treated column from step 4. Centrifuge at ≥ 6,500 x g for
      '"/>


Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. New Protocols for Isolating High- Molecular-Weight Genomic DNA
2. Eppendorf Multiporator Transfection Protocols for Eukaryotic Cells
3. Lambda DNA Extraction Protocol
4. M13/Phagemid DNA Extraction Protocol
5. Mouse Tail Genomic DNA Isolation Protocol(1)
6. Genomic DNA Isolation Protocol(1,2,3)
7. Basic Plasmid DNA Isolation Protocol
8. Protocol for RNA Isolation Using TRIzol Reagent with Phase Lock Gel-Heavy
9. RNA-free Plasmid DNA Isolation Protocol
10. Lambda DNA Extraction Protocol
11. Eppendorf Multiporator Transfection Protocols for Eukaryotic Cells
Post Your Comments:
(Date:12/17/2014)... CA (PRWEB) December 17, 2014 Once ... to provide a larger packaged waste water treatment plant ... King Abdullah Economic City (KAEC). Two years ago Bioshaft ... Jeddah Gate Development, serving two residential towers with an ... is over a quarter million gallons per day and ...
(Date:12/17/2014)... 2014 Gene synthesis and bioengineering ... entered into a technology access and services agreement ... DNA2.0’s proprietary protein engineering technology, known as “ProteinGPS®” ... are extremely excited that the global leader ADM ... proprietary bioengineering technology has now been validated through ...
(Date:12/17/2014)... , Dec. 17, 2014  United Therapeutics Corporation (NASDAQ: ... promotions as well as changes to Martine Rothblatt ... Promotions United Therapeutics announced the promotion ... Co-Chief Executive Officer and David Zaccardelli , Pharm.D. ... connection with these promotions, Dr. Rothblatt,s title will change ...
(Date:12/17/2014)...   Synageva BioPharma Corp. (NASDAQ: ... rare disorders, announced today its presentation at the upcoming ... San Francisco, CA. Sanj ... present on Monday, January 12, 2015, at 9:00 a.m. ... live and may be accessed from the "Webcasts & ...
Breaking Biology Technology:ADM and DNA2.0 Enter Into Protein Engineering Technology Access and Service Agreement 2United Therapeutics Corporation Announces Executive Promotions and CEO Compensation Change 2United Therapeutics Corporation Announces Executive Promotions and CEO Compensation Change 3United Therapeutics Corporation Announces Executive Promotions and CEO Compensation Change 4
... LEXINGTON, Mass., Sept. 29 RainDance,Technologies announced ... a,research initiative conducted with sanofi-aventis, Paris (EURONEXT: ... of Strasbourg, France, to develop,the next generation ... The consortium was founded with the assistance ...
... Sept. 29 diaDexus, Inc.,today announced its partnership ... dedicated to combating cardiovascular disease in,current and retired ... as part,of the LHF,s cardiovascular risk assessment and ... risk for suffering a heart,attack or stroke., ...
... Poniard,Pharmaceuticals, Inc. (Nasdaq: PARD ), a biopharmaceutical ... chairman and CEO,will present at the JMP Securities Healthcare ... Time at Le Parker Meridien in New York., ... discussion,of the development of picoplatin, the Company,s lead product ...
Cached Biology Technology:RainDance Technologies Signs a Collaboration Agreement with sanofi-aventis and Louis Pasteur University to Launch dScreen Consortium within ALSACE BIOVALLEY cluster 2RainDance Technologies Signs a Collaboration Agreement with sanofi-aventis and Louis Pasteur University to Launch dScreen Consortium within ALSACE BIOVALLEY cluster 3RainDance Technologies Signs a Collaboration Agreement with sanofi-aventis and Louis Pasteur University to Launch dScreen Consortium within ALSACE BIOVALLEY cluster 4RainDance Technologies Signs a Collaboration Agreement with sanofi-aventis and Louis Pasteur University to Launch dScreen Consortium within ALSACE BIOVALLEY cluster 5diaDexus, Inc. and Living Heart Foundation Offer the PLAC(R) Test to Detect Hidden Risk for Heart Attack and Stroke in Retired NFL Players 2Poniard Pharmaceuticals to Present at the JMP Securities Healthcare Focus Conference 2
(Date:12/5/2014)... 4, 2014  Tute Genomics, a leader in cloud-based ... Series A1 funding led by UK-based Eurovestech. Peak Ventures ... the investment round. "We are at a ... next-generation sequencing and seeks new approaches for the diagnosis, ... , MD MBA, and CEO of Tute Genomics. "One ...
(Date:12/3/2014)... , Dec. 2, 2014   Marvin Test ... innovative test solutions for military, aerospace, and manufacturing ... of its successful TS-900 PXI semiconductor test ... features of high-end systems to customers at a ... compared to traditional ATE. "Our ...
(Date:11/21/2014)... -- According to a new market research ... Access Control, Intrusion Detection, Parking Management, Under Vehicle Inspection), ... Geography - Global Forecasts to 2020", published by MarketsandMarkets, ... around $25 Billion in 2014 and is expected to ... of 8.69%. Browse 116 market data Tables ...
Breaking Biology News(10 mins):Tute Genomics Raises $2.3 Million to Scale Up Cloud-Based Genome Analytics and Accelerate Personalized Medicine 2Tute Genomics Raises $2.3 Million to Scale Up Cloud-Based Genome Analytics and Accelerate Personalized Medicine 3Marvin Test Solutions Brings New Capabilities to PXI-Based Semiconductor Test with TS-960 2Marvin Test Solutions Brings New Capabilities to PXI-Based Semiconductor Test with TS-960 3Industrial Security Systems Market Worth $38 Billion by 2020 2Industrial Security Systems Market Worth $38 Billion by 2020 3
... November 13, 2012Following an infection, dysregulation of the immune ... an often fatal condition called severe sepsis or septic ... underlying immune dysfunction remain poorly understood. Regulatory T cells ... to have an important role in suppressing the immune ...
... some glaciers of the Himalayas will continue shrinking for ... Young University geology professor Summer Rupper comes after her ... the monsoonal Himalayas. Published in Geophysical Research Letters ... climate remained steady, almost 10 percent of Bhutan,s glaciers ...
... PHILADELPHIA Passing one,s genes on to the next generation ... that the body would work to ensure that the genes ... New research by biologists at the University of ... the body does exactly that. This protective role is fulfilled ...
Cached Biology News:Are we closer to understanding the cause of deadly sepsis? 2Himalayan glaciers will shrink even if temperatures hold steady 2A class of RNA molecules protects germ cells from damage, Penn vet researchers show 2A class of RNA molecules protects germ cells from damage, Penn vet researchers show 3
...
... kit allows for rapid, convenient, and efficient ... bind/wash/elute process utilizes a spin column to ... for concentration of proteins which were electro-eluted ... strong ion exchangers, offering high binding capacity ...
... techniques, the AutoTrace SPE is a powerful, high-throughput workstation dedicated specifically for automating ... elution steps for large volumes aqueous solution extractions (up to 2000ml's). ... ... ...
... Disposable. Virgin, optically clear polystyrene ... and alphanumeric coordinates for cell counting. ... number is a new product number, ... number. If showing no availability yet, ...
Biology Products: