Navigation Links
Protocol for GenomePlex Whole Genome Amplification ,,,from Buccal Swab

Binding Solution to 100 l of the PCR reaction. Transfer the solution into the binding column. Centrifuge the column at maximum speed (12,000-16,000 X g) for 1 minute. Discard the eluate, but retain the collection tube.

  • Replace the binding column into the collection tube. Apply 0.5 ml of diluted Wash Solution to the column and centrifuge at maximum speed for 1 minute. Discard the eluate, but retain the collection tube.

    Note: Be sure to add ethanol to the Wash Solution Concentrate prior to first time use. See Preparation Instructions.

  • Replace the column into the collection tube. Centrifuge the column at maximum speed for 2 minutes, without any additional wash solution, to remove excess ethanol. Discard any residual eluate as well as the collection tube.

  • Transfer the column to a fresh 2 ml collection tube. Apply 50 l of Elution Solution or water to the center of each column. Incubate at room temperature for 1 minute.

    Note: When eluting with water, make sure that the pH of the water is between 5.5 and 8.5. Elution may also be performed using the Elution Solution diluted 10-fold with water.

  • To elute the DNA, centrifuge the column at maximum speed for 1 minute. The PCR amplification product is now present in the eluate and is ready for immediate use or storage at -20 C.

    Appendix

    Application Data

    Lanes 1, 7: 1kb ladder
    Lane 2: blood
    Lane 3: plant
    Lane 4: buccal swab
    Lane 5: soil
    Lane 6: positive control

    References
    1. Barker, D. L., et al. Two methods of whole-genome amplification enable accurate genotyping across a 2320-SNP linkage panel. Genomic Research, 14, 901-7 (2004).

    2. Gribble, S., et al. Chromosome paints from single cop
    '"/>



    • Source:


    Page: All 1 2 3 4 5 6

    Related biology technology :

    1. New Protocols for Isolating High- Molecular-Weight Genomic DNA
    2. Eppendorf Multiporator Transfection Protocols for Eukaryotic Cells
    3. Lambda DNA Extraction Protocol
    4. M13/Phagemid DNA Extraction Protocol
    5. Mouse Tail Genomic DNA Isolation Protocol(1)
    6. Genomic DNA Isolation Protocol(1,2,3)
    7. Basic Plasmid DNA Isolation Protocol
    8. Protocol for RNA Isolation Using TRIzol Reagent with Phase Lock Gel-Heavy
    9. RNA-free Plasmid DNA Isolation Protocol
    10. Lambda DNA Extraction Protocol
    11. Eppendorf Multiporator Transfection Protocols for Eukaryotic Cells
    Post Your Comments:
    (Date:8/20/2008)...FirstCall/ -- Eli Lilly and,Company (NYSE: LLY ) ...ion,of SGX Pharmaceuticals, Inc. (Nasdaq: SGXP ),...n drug discovery and development in the area,of on...s Special,Stockholders Meeting, in which a majorit... agreement with Lilly. Under the agreement, Lilly ...
    (Date:8/20/2008)... and Caregivers, Grassroots ...ewswire-USNewswire/ -- Inc. Magazine has,named Sen...an for,low-income seniors, the number one fastest-...rt of Inc.,s annual list of the country,s 500 fast...1,000 percent from 2004 to 2007., "This is a rewa...
    (Date:8/20/2008)...ll/ -- CEL-SCI CORPORATION,(Amex: CVM ) reports f...ing,June 30, 2008., The Company,s loss from opera...76,679 versus a loss of $3,776,263 during the same...ns for the nine months ended June 30, 2008,was $7,...e nine months in,2007., The net loss per common s...
    (Date:8/20/2008).../ -- Huifeng Bio-Pharmaceutical,Technology, Inc (O...producer of plant extracts and pharmaceutical raw ... and food production, today announced it will,be i... August 19-21 in Rio,de Janeiro, Brazil., "Unpara...volved in the,pharmaceutical raw materials industr...
    Breaking Biology Technology:Lilly Announces Completion of SGX Acquisition 2Inc. Magazine Names Senior Whole Health the Number One Fastest-Growing Private Company in US 2Inc. Magazine Names Senior Whole Health the Number One Fastest-Growing Private Company in US 3CEL-SCI CORPORATION Reports Third Quarter 2008 Financial Results 2CEL-SCI CORPORATION Reports Third Quarter 2008 Financial Results 3CEL-SCI CORPORATION Reports Third Quarter 2008 Financial Results 4CEL-SCI CORPORATION Reports Third Quarter 2008 Financial Results 5Huifeng Bio-Pharmaceutical (HFGB) to Attend CPhI South America 2008 Pharmaceutical Conference; The First CPhI Conference to be held in the Emerging Market of Brazil 2
    ...only card American businesses have left to play if...d by China, and chief information officers must ta...thor and business innovator Tom Peters. , ,Peters ...nnovation machine" to a gathering of Wisconsin bus...nference in Milwaukee. During his speaking career,...
    ...t has partnered with Medical Numerics Inc. in Virg...onance imaging software product. , ,"Both of our g...al practice," said Doug Tucker, CEO of Neurognost...borate on our efforts to develop clinically useful...l community." , , Medical Numerics specializes in...
    ...tute for Discovery has drawn the largest individua... $50 million from John and Tashia Morgridge. , ,Th...in Alumni Research Foundation, and the State Build...tate as well, officials announced on Monday. , ,Jo...ms and a UW-Madison alumnus, said in a release tha...
    ... dismal 16th worldwide in broadband deployment lar...y, a member of the Federal Communications Commissi...al Midwestern Telecommunications and Technology Co... Michael J. Copps said the nation has established ...h a strategy to meet them. , ,"I,m waiting to see ...
    Other Biology Technology:Peters: Innovation the only edge that remains for American business 2Morgridge gift enables phase one of Institute for Discovery 2Broadband access needs a champion, FCC commissioner says 2Broadband access needs a champion, FCC commissioner says 3
    (Date:8/20/2008)...tre for Addiction and Mental Health (CAMH) is prou... by the Canada Foundation for Innovation (CFI) int...ervention, treatment and prevention of mental illn... , This funding from the Large-Scale Institutio...l Fund will allow CAMH to leverage our scientific ...
    (Date:8/20/2008)...LUMBUS, Ohio -- Researchers here have found a way ...n very efficiently. , A new catalyst makes hydro...ble temperature, and using inexpensive ingredients...ecular engineering at Ohio State University, said ...others being developed around the world, because i...
    (Date:8/19/2008)...rosting isnt just for livening up boring bran flak...teins. Researchers at the National Institute of St...t, inexpensive and effective method for evaluating...ize protein-drugs for storage at room temperature....American Chemical Society National Meeting and Exp...
    (Date:8/19/2008)...ction that often goes undetected can block the lun...oke, according to researchers at National Jewish H... scheduled to appear in the October issue of Infe...y cause smokers to develop chronic obstructive pul...helming cause of chronic obstructive pulmonary dis...
    Breaking Biology News(10 mins):Large-scale investment catapults CAMH's mental illness and addiction research forward 2A better way to make hydrogen from biofuels 2A better way to make hydrogen from biofuels 3Candy-coating keeps proteins sweet 2Infection blocks lung's protective response against tobacco smoke 2Members of European Parliament discuss food labeling and heart health 20909 1Members of European Parliament discuss food labeling and heart health 20909 2Pussycat Dolls Shape Up With Bally Total Fitness 20906 1Pussycat Dolls Shape Up With Bally Total Fitness 20906 2Rigel to Host Conference Call to Discuss Preliminary Phase 2 Study Results of R788 in Lymphoma 2298 1Rigel to Host Conference Call to Discuss Preliminary Phase 2 Study Results of R788 in Lymphoma 2298 2Before Every Surgery Take a TIME OUT 28R 29 5745 1Before Every Surgery Take a TIME OUT 28R 29 5745 2
    ...continual and inevitable shortening of telomeres, ...hromosomes, has been linked to aging and physical ...are more ways than one to grow old. , Researchers ...te for the first time that the roundworm Caenorhab...though its telomeres are still long, and moves wit...
    ...the first time, researchers have observed exactly ...d by the sun,s ultraviolet (UV) radiation. , The O... photolyase uses energy from visible light to repa...mals, including humans, although all plants and al...how photolyase works could one day lead to drugs t...
    ...archers at UT Southwestern Medical Center have fou...s of cell death ?a beneficial and normal process w...cancers when it malfunctions. , The researchers a...y to determine how mutations in the enzyme’s gene ...eally be a hot spot in research,?said Dr. Qing Zho...
    ...arch led by biologists at the University of Califo...DNA sets off a chain reaction that leads to the in...ody,s immune system. , The new findings, to be pub...e, shed light on a long-standing question of how t...ttack tumors - can differentiate cells that are ca...
    Other Biology News:Tiny roundworm's telomeres help scientists to tease apart different types of aging 2Tiny roundworm's telomeres help scientists to tease apart different types of aging 3Scientists get first glimpse at how plants, most animals repair UV-damaged DNA 2Scientists get first glimpse at how plants, most animals repair UV-damaged DNA 3UT Southwestern researchers discover master switch in cell death 2Renewed hope for Parkinson's patients 2Renewed hope for Parkinson's patients 3
    ...integrins in vascular proliferation, adhesion, and...rin family of cell adhesion receptors consists of ...osed of an a and b subunit that associate in a non...ersity of the integrin family are based upon the p...
    siTrio siRNA --- a cocktail of 3 siRNAs specifically targeted to your target gene of interestis our most popular product. The siTrio reagent guarantees you get more than 75% knockdown of your target
    siTrio siRNA --- a cocktail of 3 siRNAs specifically targeted to your target gene of interestis our most popular product. The siTrio reagent guarantees you get more than 75% knockdown of your target
    ... improved stability and convenience from the forme...as overcome this problem but also produced some ad... formulation of HRP conjugated antibody from lyoph...ge, the stability of the conjugate has improved so...
    Biology Products: