Navigation Links
Protocol for GenomePlex Whole Genome Amplification ,,,from Buccal Swab

ube.
  • Add 500 l of Wash Solution (be sure to dilute with ethanol prior to first use) and centrifuge at ≥ 6,500 X g for 1 minute.
  • Discard the collection tube containing the flow through and place the binding column in a new 2 ml collection tube.
  • Add another 500 l of Wash Solution to the binding column and centrifuge at maximum speed (12,000 X g to 16,000 X g) for 3 minutes to dry the binding column.
  • Pipette 200 l of Elution Solution onto the binding column and centrifuge for 1 minute at ≥ 6,500 X g.
  • Store the eluted DNA at - 20 C.

    V. Protocol for GenomePlex Whole Genome Amplification from Buccal Swab

    • Performed with GenomePlex Whole Genome Amplification Kit (WGA1)

      Fragmentation

      1. Prepare DNA solution of 1 ng/l from buccal swab extraction protocol described above.
      2. Add 1 l of 10X Fragmentation Buffer to 10 l DNA (1 ng/l) in a PCR tube.
      3. Place the tube in a thermal cycler at 95 C for EXACTLY 4 minutes. Note, the incubation is time sensitive and any deviation may alter results.
      4. Immediately cool the sample on ice and centrifuge briefly.

      Library Preparation

      1. Add 2 l of 1x Library Preparation Buffer.
      2. Add 1 l of Library Stabilization Solution.
      3. Mix thoroughly and place in thermal cycler at 95 C for 2 minutes.
      4. Cool the sample on ice and centrifuge briefly.
      5. Add 1 l Library Preparation Enzyme, mix thoroughly, and centrifuge briefly.
      6. Place sample in thermal cycler and incubate as follows:
        • 16 C for 20 minutes
        • 24 C for 20 minutes
        • 37 C for 20 minutes
        • 75 C for 5 minutes
        • 4 C hold
      7. Remove samples from thermal cycler and centrifuge briefly. Samples may be amplified immediately or stored at - 20 C
        '"/>


    Source:


    Page: All 1 2 3 4 5 6

    Related biology technology :

    1. New Protocols for Isolating High- Molecular-Weight Genomic DNA
    2. Eppendorf Multiporator Transfection Protocols for Eukaryotic Cells
    3. Lambda DNA Extraction Protocol
    4. M13/Phagemid DNA Extraction Protocol
    5. Mouse Tail Genomic DNA Isolation Protocol(1)
    6. Genomic DNA Isolation Protocol(1,2,3)
    7. Basic Plasmid DNA Isolation Protocol
    8. Protocol for RNA Isolation Using TRIzol Reagent with Phase Lock Gel-Heavy
    9. RNA-free Plasmid DNA Isolation Protocol
    10. Lambda DNA Extraction Protocol
    11. Eppendorf Multiporator Transfection Protocols for Eukaryotic Cells
  • Post Your Comments:
    (Date:8/21/2014)... Dallas, Texas (PRWEB) August 21, 2014 ... Global Seaweed Fertilizer Industry” is a professional ... Fertilizer market. The report provides basic Seaweed ... application, and industry chain structure as well ... international market analysis, including domestic market as ...
    (Date:8/21/2014)... August 21, 2014 The College of ... private college in the Mid-Hudson Region to be designated ... begin accepting applications from qualified “high-technology” businesses that align ... very pleased to have been selected for the START-UP ... transformative initiative to stimulate economic development in New York ...
    (Date:8/21/2014)... HONG KONG , Aug. 21, 2014  China Cord ... today announced its plan to release financial results for ... August 28, 2014, after market close in the US.  ... call at 8:00 a.m. ET on Friday, August 29, ... brief overview of the Company,s recent developments, followed by ...
    (Date:8/21/2014)... August 21, 2014 Gallus ... contract development and manufacturing organization (CMO) announced today ... Inc., an oncology company focused on the development ... to manufacture the anti-prostate specific membrane antigen (PSMA) ... candidate. Under the agreement the antibody will ...
    Breaking Biology Technology:Internationals Seaweed Fertilizer Industry 2014 Development Trend Analysis in New Research Study at DeepResearchReports.com 2Internationals Seaweed Fertilizer Industry 2014 Development Trend Analysis in New Research Study at DeepResearchReports.com 3The College of New Rochelle Becomes First Private College in Mid-Hudson Region to Earn START-UP NY Designation 2The College of New Rochelle Becomes First Private College in Mid-Hudson Region to Earn START-UP NY Designation 3China Cord Blood Corporation to Report First Quarter of Fiscal 2015 Financial Results 2Gallus Enters Agreement with Progenics Pharmaceuticals, Inc. for Manufacture of Anti-PSMA Monoclonal Antibody 2Gallus Enters Agreement with Progenics Pharmaceuticals, Inc. for Manufacture of Anti-PSMA Monoclonal Antibody 3
    ... MILWAUKEE--- GE Healthcare , a $10 billion provider ... information technology, announced today that it has received ... healthcare industry authority, KLAS Enterprises for the second ... called Centricity(R) Pharmacy Information System, was recognized in ...
    ... OASYS , a leading developer of K-12 software ... Behind management, recently announced their online, ASP software ... (IEPs) and monitors the progress of children with ... in 16 Wisconsin school districts during 2003, provides ...
    ... Third Wave Technologies Inc , a maker of molecular ... 2D6 analysis panel, which provides clinical trial laboratories with ... for the important 2D6 gene. , ,The panel is ... the ability to configure custom assay panels. The panel ...
    Cached Biology Technology:Third Wave Releases Gene Test 2
    (Date:8/22/2014)... be able to purchase fuel cell cars from Toyota ... of the cars will run on hydrogen made from ... warming. , Now scientists at Stanford University have developed ... battery to produce hydrogen by water electrolysis. The ... split liquid water into hydrogen and oxygen gas. Unlike ...
    (Date:8/21/2014)... of American singles found that during sex with ... rates. On average, men experience orgasm 85.1 percent ... little difference. For women, however, orgasm occurrence is ... percent of the time during sex with a ... women,s sexual orientation, with lesbian women experiencing orgasm ...
    (Date:8/21/2014)... dandruff, eczema and other itchy, flaky maladies in humans ... Hawaiian coral reefs and the extreme environments of arctic ... the scientific journal PLOS Pathogens considers the ... genus Malassezia in light of new insights ... world. , University of Hawai,i at Mānoa scientist Anthony ...
    Breaking Biology News(10 mins):Stanford scientists develop a water splitter that runs on an ordinary AAA battery 2Stanford scientists develop a water splitter that runs on an ordinary AAA battery 3Orgasm rates for single women less predictable than men's, vary by sexual orientation 2Orgasm rates for single women less predictable than men's, vary by sexual orientation 3Orgasm rates for single women less predictable than men's, vary by sexual orientation 4From dandruff to deep sea vents, an ecologically hyper-diverse fungus 2
    ... common contact allergy in adults. Research at the University ... which many deem harmless compared to synthetic perfumes, may ... adults in northern Europe is believed to suffer from ... common is nickel allergy, but many people also suffer ...
    ... Your mother always told you not to use your teeth ... Human skulls have small faces and teeth and are not ... of our earliest ancestors, say scientists. New research published in ... Academy of Sciences reveals nut-cracking abilities in our 250-million-year-old ...
    ... discovered a chemical that stops stem cells from turning into ... develop new medical treatments more easily. Stem cells have ... the body, and scientists believe they have huge potential to ... Professor Melanie Welham,s team at the University of ...
    Cached Biology News:Even natural perfumes may cause allergies 2Early humans had 'jaws of steel' 2Don't go changing: New chemical keeps stem cells young 2
    EDG-1CT (endothelial cell differentiation gene-1, C terminal)...
    Mouse Anti-human CD11b/Mac-1alpha, clone LM2/1, Monoclonal Antibody...
    Mouse monoclonal [1-N-14] to Adenovirus Type 7 hexon C11 Immunogen: Purified Adenovirus type 7 Specificity: This antibody is specific for Adenovirus type 7 hexon C11....
    ... Kir2.1 protein. The epitope is specific ... in any other known protein.,SPECIES REACTIVITIES: ... in rabbit, bovine, porcine and guinea ... rat and mouse (17/19 residues) and ...
    Biology Products: