II. Product Components
III. Materials to be Supplied by the User
IV. Protocol for Extraction of DNA from Buccal Swab
V. Protocol for GenomePlex Whole Genome Amplification from Buccal Swab
VI. Quantification of Amplified Products
VII. Purification of Amplified Products
This protocol provides a simple and convenient method to isolate, amplify, and purify genomic DNA from buccal swabs. Samples that result in low quantity genomic DNA can hinder the researcher's ability to perform downstream analysis. The protocol presents a simple and accurate tool to immortalize DNA for downstream applications. This method describes the means for amplifying nanogram amounts of starting genomic DNA with little or no detectable bias resulting in microgram quantities. The protocol is completed using the GenElute Mammalian Genomic DNA MiniPrep Kit, GenomePlex WGA kit, and GenElute PCR Clean-up Kit.
GenomePlex is a Whole Genome Amplification (WGA) method that allows the researcher to generate a representative, approximate 1000-fold amplification of genomic DNA. The kit utilizes a proprietary amplification technology based upon random fragmentation of genomic DNA and conversion of the resulting small fragments to PCR-amplifiable OmniPlex Library molecules flanked by universal priming sites. The OmniPlex library is then PCR amplified using universal oligonucleotide primers and a limited number of cycles.
This technology maintains the genetic representation with conco