Protocol for GenomePlex Whole Genome Amplification ,,,from Whole Blood ( 16000 Xg) for 1 minute. Discard the el...)

Protocol for GenomePlex Whole Genome Amplification ,,,from Whole Blood

16,000 Xg) for 1 minute. Discard the eluate, but retain the collection tube.

Replace the binding column into the collection tube. Apply 0.5 ml of diluted Wash Solution to the column and centrifuge at maximum speed for 1 minute. Discard the eluate, but retain the collection tube.

Note: Be sure to add ethanol to the Wash Solution Concentrate prior to first time use. See Preparation Instructions.

Replace the column into the collection tube. Centrifuge the column at maximum speed for 2 minutes, without any additional wash solution, to remove excess ethanol. Discard any residual eluate as well as the collection tube.

Transfer the column to a fresh 2 ml collection tube. Apply 50 l of Elution Solution or water to the center of each column. Incubate at room temperature for 1 minute.

Note: When eluting with water, make sure that the pH of the water is between 5.5 and 8.5. Elution may also be performed using the Elution Solution diluted 10-fold with water.

To elute the DNA, centrifuge the column at maximum speed for 1 minute. The PCR amplification product is now present in the eluate and is ready for immediate use or storage at -20 C.

Appendix

Application Data

Performance of DNA Amplified with GenomePlex WGA Identical to Non-Amplified DNA

A blood sample was isolated for genomic DNA using the GenElute Blood Genomic DNA Kit (NA2000). 10ng of genomic DNA was amplified using the GenomePlex WGA kit (WGA1) followed by purification using the GenElute PCR Clean Up Kit. SNP genotyping analysis was performed on non-amplified DNA and GenomePlex amplified DNA. GenomePlex WGA DNA genotyping results provided the same accuracy and quality of scores to non-amplified DNA.

References
1. Barker, D. L., et al. Two methods of whole-gen
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