Navigation Links
Protocol for GenomePlex Whole Genome Amplification ,,,from Whole Blood

  • Add the following reagents to the entire 15 l reaction:
    • 7.5 l 10x Amplification Master Mix
    • 47.5 l Nuclease Free Water
    • 5.0 l JumpStart Taq DNA Polymerase (12.5 units)
  • Mix thoroughly, centrifuge briefly, and begin thermocycling:
      Initial Denaturation 95 C for 3 minutes
      Perform 14 cycles as follows:
    • Denature 95 C for 15 seconds
    • Anneal/Extend 65 C for 5 minutes
  • After cycling is complete, maintain the reactions at 4 C or store at -20 C until ready for analysis or purification. VI. Quantification of Amplified Products
    The amount of GenomePlex Whole Genome Amplification Kit products can be detected with or without purification. For the highest quality samples of DNA we strongly recommend cleaning up the samples after amplification. The amplified products can be measured with the PicoGreen dsDNA Quantitation Assay (Molecular Probes Inc. Product # P-7589). Another method of detecting the amplified products is spectrophotometric absorption (OD260) on a NanoDrop instrument.

    VII. Purification of Amplified Products

    • Performed with GenElute PCR Clean-Up Kit (NA1020)
      1. Insert a GenElute Miniprep Binding Column (with a blue o-ring) into a provided collection tube, if not already assembled. Add 0.5 ml of the Column Preparation Solution to each miniprep column and centrifuge at 12,000 x g for 30 seconds to 1 minute. Discard the eluate.

        Note: The Column Preparation Solution maximizes binding of the DNA to the membrane resulting in more consistent yields.

      2. Add 5 volumes of Binding Solution to 1 volume of the PCR reaction and mix. For example, add 500 l of Binding Solution to 100 l of the PCR reaction. Transfer the solution into the binding column. Centrifuge the column at maximum speed (12,000-
        '"/>


    Source:


    Page: All 1 2 3 4 5 6

    Related biology technology :

    1. New Protocols for Isolating High- Molecular-Weight Genomic DNA
    2. Eppendorf Multiporator Transfection Protocols for Eukaryotic Cells
    3. Lambda DNA Extraction Protocol
    4. M13/Phagemid DNA Extraction Protocol
    5. Mouse Tail Genomic DNA Isolation Protocol(1)
    6. Genomic DNA Isolation Protocol(1,2,3)
    7. Basic Plasmid DNA Isolation Protocol
    8. Protocol for RNA Isolation Using TRIzol Reagent with Phase Lock Gel-Heavy
    9. RNA-free Plasmid DNA Isolation Protocol
    10. Lambda DNA Extraction Protocol
    11. Eppendorf Multiporator Transfection Protocols for Eukaryotic Cells
  • Post Your Comments:
    (Date:11/22/2014)... Grand Rapids, MI (PRWEB) November 22, 2014 ... the opportunity to simulate the proper management of ... training and test lung devices . Grand Rapids-based, ... Lung” plans to donate two of the respiratory ... $12,000 to $15,000 each. Grand Valley and Muskegon ...
    (Date:11/21/2014)...   TRU-D SmartUVC LLC and its UK partners, ... disinfection robot, TRU-D SmartUVC, at FIS 2014 in ... 26 at Stand 23. The conference, hosted by ... largest infection-related event of its kind and brings ... the leading events of its type throughout the world. This ...
    (Date:11/21/2014)... Alto, Calif. (PRWEB) November 20, 2014 ... a nonprofit, public policy research organization representing leading ... pharmaceutical firms, and PwC US today ... states in life sciences industry growth. The trend ... 2013. The 2015 California Biomedical Industry Report ...
    (Date:11/21/2014)... Angeles, CA (PRWEB) November 21, 2014 ... it that makes Albert Einstein so instantly recognizable? Why ... African-born physicist, mathematician, astronomer and author Hilton Ratcliffe seeks ... they have nothing at all to do with science. ... Muse Harbor Publishing , November 21, 2014), Ratcliffe puts ...
    Breaking Biology Technology:Michigan Instruments Donates Two Respiratory Simulation Training and Test Lung (TTL) Devices to Grand Valley State University 2Michigan Instruments Donates Two Respiratory Simulation Training and Test Lung (TTL) Devices to Grand Valley State University 3TRU-D SmartUVC to Showcase Superbug-Killing Robot at FIS 2014 2TRU-D SmartUVC to Showcase Superbug-Killing Robot at FIS 2014 3California Healthcare Institute and PwC Report Boom in California’s Biomedical Industry 2California Healthcare Institute and PwC Report Boom in California’s Biomedical Industry 3California Healthcare Institute and PwC Report Boom in California’s Biomedical Industry 4“Stephen Hawking Smoked My Socks” Sparks Controversy in Science World 2“Stephen Hawking Smoked My Socks” Sparks Controversy in Science World 3“Stephen Hawking Smoked My Socks” Sparks Controversy in Science World 4
    ... Empowers Users to Take Full Control of the ... to Results Disclosure - while Adhering to Changing ... to Manage Their Clinical Trial Registration and Results ... ( http://www.intrasphere.com ), a ...
    ... , - European Operating ... Healthcare Educational Program, Accepted,for Accreditation , ... healthcare barrier,protective solutions, has announced it has taken a ... the area of continued,education. , ...
    ... developed an entirely new method for starting chemical reactions. ... control catalytic activity one of the most fundamental ... reactions with mechanical force. This discovery paves the way ... influence of mechanical tension. The results of their research ...
    Cached Biology Technology:Intrasphere Technologies Releases Version 1.5 of Its PharmaCM Clinical Trial Registration Solution Powered by Microsoft 2Intrasphere Technologies Releases Version 1.5 of Its PharmaCM Clinical Trial Registration Solution Powered by Microsoft 3Intrasphere Technologies Releases Version 1.5 of Its PharmaCM Clinical Trial Registration Solution Powered by Microsoft 4Ansell Healthcare Espresses Firm Commitment to Support Continued Education of Healthcare Professionals Worldwide 2Ansell Healthcare Espresses Firm Commitment to Support Continued Education of Healthcare Professionals Worldwide 3Ansell Healthcare Espresses Firm Commitment to Support Continued Education of Healthcare Professionals Worldwide 4A molecular ripcord for chemical reactions 2
    (Date:11/3/2014)... Australia report that low birth weight and preterm birth ... replacements in adulthood. Findings published in the American College ... , indicate that low birth weight and pre-term babies ... to OA as adults. , According to the ... are diagnosed with clinical OA. Symptoms of OA range ...
    (Date:11/2/2014)... November 2014)—In support of a bold quest to rid ... Gates Foundation today announced an award of US$156 million ... (MVI) in building new vaccines that will interrupt the ... "accelerating to zero" agenda. Such vaccines would ensure that ... called an "immunological bed net." , This approach ...
    (Date:11/2/2014)... walk into a bar and bump into a biologist . ... to some late-night nerd sketch, researchers have taken this premise ... biology, namely, finding meaning in the rising oceans of genomic ... cancer mutations that genome-wide studies are publishing at a dizzying ... parse the signal from the noise (and there is no ...
    Breaking Biology News(10 mins):Preterm, low birth-weight babies may need new hips in adulthood 2New malaria vaccines to prevent infection and block transmission get a shot in the arm 2New malaria vaccines to prevent infection and block transmission get a shot in the arm 3New malaria vaccines to prevent infection and block transmission get a shot in the arm 4Mutant models 2Mutant models 3Mutant models 4
    ... of some insects or the deep black of a ... the nanostructure of a fly,s eyes, according to Penn ... and inexpensively copy biological surface structures. "Only a ... says Akhlesh Lakhtakia, the Charles Godfrey Binder (Endowed) Professor ...
    ... size guidelines for all-terrain vehicles (ATV) are inadequate to ... results from a study by researchers at the University ... found that national ATV size guidelines for youth ... frame or engine size do not assure a ...
    ... of sedentary and overweight older people placed on a four-month ... more fat, compared to older sedentary people who were placed ... study also showed that when older people diet without exercising, ... said senior researcher Bret H. Goodpaster. When they combined weight ...
    Cached Biology News:Coating copies microscopic biological surfaces 2Coating copies microscopic biological surfaces 3Study finds ATV guidelines inadequate 2Older people who diet without exercising lose valuable muscle mass 2