Navigation Links
Protocol for GenomePlex Whole Genome Amplification ,,,from Whole Blood

  • Add the following reagents to the entire 15 l reaction:
    • 7.5 l 10x Amplification Master Mix
    • 47.5 l Nuclease Free Water
    • 5.0 l JumpStart Taq DNA Polymerase (12.5 units)
  • Mix thoroughly, centrifuge briefly, and begin thermocycling:
      Initial Denaturation 95 C for 3 minutes
      Perform 14 cycles as follows:
    • Denature 95 C for 15 seconds
    • Anneal/Extend 65 C for 5 minutes
  • After cycling is complete, maintain the reactions at 4 C or store at -20 C until ready for analysis or purification. VI. Quantification of Amplified Products
    The amount of GenomePlex Whole Genome Amplification Kit products can be detected with or without purification. For the highest quality samples of DNA we strongly recommend cleaning up the samples after amplification. The amplified products can be measured with the PicoGreen dsDNA Quantitation Assay (Molecular Probes Inc. Product # P-7589). Another method of detecting the amplified products is spectrophotometric absorption (OD260) on a NanoDrop instrument.

    VII. Purification of Amplified Products

    • Performed with GenElute PCR Clean-Up Kit (NA1020)
      1. Insert a GenElute Miniprep Binding Column (with a blue o-ring) into a provided collection tube, if not already assembled. Add 0.5 ml of the Column Preparation Solution to each miniprep column and centrifuge at 12,000 x g for 30 seconds to 1 minute. Discard the eluate.

        Note: The Column Preparation Solution maximizes binding of the DNA to the membrane resulting in more consistent yields.

      2. Add 5 volumes of Binding Solution to 1 volume of the PCR reaction and mix. For example, add 500 l of Binding Solution to 100 l of the PCR reaction. Transfer the solution into the binding column. Centrifuge the column at maximum speed (12,000-
        '"/>


    Source:


    Page: All 1 2 3 4 5 6

    Related biology technology :

    1. New Protocols for Isolating High- Molecular-Weight Genomic DNA
    2. Eppendorf Multiporator Transfection Protocols for Eukaryotic Cells
    3. Lambda DNA Extraction Protocol
    4. M13/Phagemid DNA Extraction Protocol
    5. Mouse Tail Genomic DNA Isolation Protocol(1)
    6. Genomic DNA Isolation Protocol(1,2,3)
    7. Basic Plasmid DNA Isolation Protocol
    8. Protocol for RNA Isolation Using TRIzol Reagent with Phase Lock Gel-Heavy
    9. RNA-free Plasmid DNA Isolation Protocol
    10. Lambda DNA Extraction Protocol
    11. Eppendorf Multiporator Transfection Protocols for Eukaryotic Cells
  • Post Your Comments:
    (Date:8/22/2014)... a study published today (August 22nd), in the ... the Champalimaud Neuroscience Programme establish the effect of ... of advanced genetic and optical techniques., "Serotonin is ... a wide range of brain functions, from the ... of complex emotional behaviours, This neurotransmitter is also ...
    (Date:8/22/2014)... 2014 Ophthalmic drug delivery technologies ... challenging endeavors as the anatomy, physiology and biochemistry ... the foreign substances. Ocular drug delivery technologies provide ... and minimal use of needles; also it enables ... provides specific targeting within the ocular globe. On ...
    (Date:8/22/2014)... -- Research and Markets has announced the ... & Reagents (Cloning, Epigenetics, PCR, Restriction Digestion, Sequencing) Market ... The global molecular biology enzymes market ... expected to grow at a CAGR of 13.74% to ... & reagents find applications in a large number of ...
    (Date:8/22/2014)... 22, 2014 The identification of ... can be accelerated by exome sequencing. Built on ... the custom SeqCap EZ Developer system has been ... Watson will additionally present how he achieved coverage ... human and mouse exome kits. With over 236,000 ...
    Breaking Biology Technology:From happiness to pain: Understanding serotonin's function 2Ocular Drug Delivery Technologies Market: Global Industry Analysis, Size, Share, Growth, Trends and Forecast 2014 - 2020 2Ocular Drug Delivery Technologies Market: Global Industry Analysis, Size, Share, Growth, Trends and Forecast 2014 - 2020 3Ocular Drug Delivery Technologies Market: Global Industry Analysis, Size, Share, Growth, Trends and Forecast 2014 - 2020 4Global Molecular Biology Enzymes, Kits, & Reagents (Cloning, Epigenetics, PCR, Restriction Digestion, Sequencing) Market - Forecast to 2018 2Global Molecular Biology Enzymes, Kits, & Reagents (Cloning, Epigenetics, PCR, Restriction Digestion, Sequencing) Market - Forecast to 2018 3Efficient Identification of Protein-Coding Variants in a Model Organism Through Exome Sequencing, New Webinar Hosted by Xtalks 2
    ... LOS ANGELES, May 3, 2012 MD+DI ... UBM Canon,s leading brand providing the medical device industry with ... a 2012 Maggie Award for Best Overall Category in the ... 2011 issue. MD+DI,s May 2011 cover ...
    ... PDL BioPharma, Inc. (PDL) (NASDAQ: PDLI ) ... 31, 2012. (Logo:  http://photos.prnewswire.com/prnh/20110822/SF55808LOGO ) ... percent over the same period of 2011. Total revenues for ... $83.3 million for the same period of 2011. Total revenue ...
    ... May 3, 2012 Immunomic Therapeutics, Inc., ("ITI," Lancaster, ... MD, announced that the U.S. Food and Drug Administration ... Drug Application ("IND") filed for the allergy immunotherapy, JRC- ... ITI that there will be no clinical hold and ...
    Cached Biology Technology:MD+DI, the Global MedTech Industry Authority, Wins a 2012 Maggie for Best Overall Category in Medical, Dental & Related Services/Trade 2PDL BioPharma Announces First Quarter 2012 Financial Results 2PDL BioPharma Announces First Quarter 2012 Financial Results 3PDL BioPharma Announces First Quarter 2012 Financial Results 4PDL BioPharma Announces First Quarter 2012 Financial Results 5Immunomic Therapeutics Receives IND Authorization for Phase I Study of JRC-LAMP-Vax Vaccine to Treat Japanese Red Cedar (Sugi) Allergy 2Immunomic Therapeutics Receives IND Authorization for Phase I Study of JRC-LAMP-Vax Vaccine to Treat Japanese Red Cedar (Sugi) Allergy 3
    (Date:8/22/2014)... is available in German . ... in PCFs proteins are fixated in a way so ... Next, the HZB and Fudan University researchers are planning ... functional materials. Their findings are being published today in ... , Proteins are sensitive molecules. Everyone knows that ...
    (Date:8/22/2014)... American consumers will finally be able to purchase fuel ... as zero-emissions vehicles, most of the cars will run ... that contributes to global warming. , Now scientists at ... uses an ordinary AAA battery to produce hydrogen by ... through two electrodes that split liquid water into hydrogen ...
    (Date:8/21/2014)... -- A new study of American singles found ... have the highest orgasm rates. On average, men ... their sexual orientation making little difference. For women, ... women experience orgasm 62.9 percent of the time ... this pattern varies with women,s sexual orientation, with ...
    Breaking Biology News(10 mins):Proteins: New class of materials discovered 2Stanford scientists develop a water splitter that runs on an ordinary AAA battery 2Stanford scientists develop a water splitter that runs on an ordinary AAA battery 3Orgasm rates for single women less predictable than men's, vary by sexual orientation 2Orgasm rates for single women less predictable than men's, vary by sexual orientation 3Orgasm rates for single women less predictable than men's, vary by sexual orientation 4
    ... - (Toronto) A new study from the Centre for ... a variation of a specific gene may play a role ... of Alzheimer,s cases. This innovative study has combined genetics and ... developing late-onset Alzheimer,s disease long before symptoms appear. The ...
    ... examine the response to noise of a diverse range of ... found that many families of crustaceans previously assumed to be ... Lizard Island on the Great Barrier Reef, the team collected ... webs. On each of 34 nights, one light trap ...
    ... (Minority Access to Research Careers) Program has announced the ... Sports Medicine (ACSM) Southeast Chapter Annual Meeting in Greenville, ... to promote the entry of underrepresented minority students, postdoctorates ... community and to encourage the participation of young scientists ...
    Cached Biology News:Combining brain imaging, genetic analysis may help identify people at early risk of Alzheimer's 2Rowdy residents warn crustaceans away from perilous reefs 2
    ... The Label IT siRNA Tracker ... Reagent provides a straight forward approach ... of your design, in an efficient ... tracking experiments. Subcellular localization and functional ...
    ... (A) and 36kDa (C) subunits Formulation: ... 500mM NaCl, 60mM 2-mercaptoethanol, 1mM MgCl2, ... and 0.1mg/ml serum albumin Properites: For ... Purification: DEAE chromatography, aminohexyl-agarose and gel ...
    ... IT siRNA Tracker Intracellular Localization Kit ... straight forward approach to directly label ... in an efficient yet non-destructive manner, ... localization and functional inhibition of target ...
    ... The Label IT siRNA Tracker Intracellular ... provides a straight forward approach to ... your design, in an efficient yet ... experiments. Subcellular localization and functional inhibition ...
    Biology Products: