Navigation Links
Protocol for GenomePlex Whole Genome Amplification ,,,from Whole Blood

se) and centrifuge at ≥ 6,500 x g for 1 minute.
  • Discard the collection tube containing the flow-through and place the column in a new 2 ml collection tube.
  • Add 500 l of Wash Solution (be sure to dilute with ethanol prior to first use) to the binding column and centrifuge at maximum speed (12,000 x g to 16,000 x g) for 3 minutes to dry the binding column.
  • Pipette 200 l of Elution Solution onto the column and centrifuge for 1 minute at ≥ 6,500 x g to elute the DNA.
  • Store the eluted DNA at -20 C or proceed with the next step. V. Protocol for GenomePlex Whole Genome Amplification from Whole Blood
    • Performed with GenomePlex Whole Genome Amplification Kit (WGA1)

      Fragmentation

      1. Prepare DNA solution of 1 ng/l from whole blood extraction protocol described above.
      2. Add 1 l of 10X Fragmentation Buffer to 10 l DNA (1 ng/l) in a PCR tube.
      3. Place the tube in a thermal cycler at 95 C for EXACTLY 4 minutes. Note, the incubation is time sensitive and any deviation may alter results.
      4. Immediately cool the sample on ice and centrifuge briefly.

      Library Preparation

      1. Add 2 l of 1x Library Preparation Buffer.
      2. Add 1 l of Library Stabilization Solution.
      3. Mix thoroughly and place in thermal cycler at 95 C for 2 minutes.
      4. Cool the sample on ice and centrifuge briefly.
      5. Add 1 l Library Preparation Enzyme, mix thoroughly, and centrifuge briefly.
      6. Place sample in thermal cycler and incubate as follows:
        • 16 C for 20 minutes
        • 24 C for 20 minutes
        • 37 C for 20 minutes
        • 75 C for 5 minutes
        • 4 C hold
      7. Remove samples from thermal cycler and centrifuge briefly. Samples may be amplified immediately or stored at - 20 C up to three days.

      Amplification


        '"/>


    Source:


    Page: All 1 2 3 4 5 6

    Related biology technology :

    1. New Protocols for Isolating High- Molecular-Weight Genomic DNA
    2. Eppendorf Multiporator Transfection Protocols for Eukaryotic Cells
    3. Lambda DNA Extraction Protocol
    4. M13/Phagemid DNA Extraction Protocol
    5. Mouse Tail Genomic DNA Isolation Protocol(1)
    6. Genomic DNA Isolation Protocol(1,2,3)
    7. Basic Plasmid DNA Isolation Protocol
    8. Protocol for RNA Isolation Using TRIzol Reagent with Phase Lock Gel-Heavy
    9. RNA-free Plasmid DNA Isolation Protocol
    10. Lambda DNA Extraction Protocol
    11. Eppendorf Multiporator Transfection Protocols for Eukaryotic Cells
  • Post Your Comments:
    (Date:8/21/2014)... The College of New Rochelle announced today ... Mid-Hudson Region to be designated as a START-UP NY ... qualified “high-technology” businesses that align with the College’s academic ... been selected for the START-UP NY program and extremely ... economic development in New York by growing business and ...
    (Date:8/21/2014)... OTC Markets Group Inc. ... company, on its approval to list on The ... the best marketplace for established U.S. and global ... Logo - http://photos.prnewswire.com/prnh/20110118/MM31963LOGO "We ... its growth strategy and achievement of this important ...
    (Date:8/21/2014)... (PRWEB) August 21, 2014 Pursuit ... selling optimization technology and services for Life Sciences ... “Grading Pharma’s Use of New Commercial Sales Models” ... & Analytics. , The article examines some innovative ... that are being tested in the pharmaceutical market. ...
    (Date:8/21/2014)... 2014 SoundConnect , a ... as one of the nation’s Fastest Growing Private ... the 2nd consecutive year. Inc. magazine today ranked ... 500|5000, an exclusive ranking of the nation's fastest-growing ... look at the most important segment of the ...
    Breaking Biology Technology:The College of New Rochelle Becomes First Private College in Mid-Hudson Region to Earn START-UP NY Designation 2The College of New Rochelle Becomes First Private College in Mid-Hudson Region to Earn START-UP NY Designation 3OTC Markets Group Congratulates Immune Pharmaceuticals on NASDAQ Listing 2Pursuit's Peter Robinson Publishes "Grading Pharma's Use of New Commercial Sales Models" 2SoundConnect Returns on Inc. 5000 List of Fastest Growing Companies 2SoundConnect Returns on Inc. 5000 List of Fastest Growing Companies 3
    ... Dendreon Corporation (Nasdaq: DNDN ) today announced ... reviewed the impact of labeled and unlabeled use of ... advanced prostate cancer. This panel was convened as a ... initiated by the Centers for Medicare and Medicaid Services ...
    ... Nov. 17, 2010 Terso Solutions, Inc. ... cost way to control inventory management ... that can be implemented in less ... http://photos.prnewswire.com/prnh/20101117/CG03241-a ) (Photo:   http://www.newscom.com/cgi-bin/prnh/20101117/CG03241-a ...
    ... RESEARCH TRIANGLE PARK, N.C., Nov. 17, 2010 Chimerix, ... presented an update to the World Health Organization (WHO) ... CMX001, at the 12th Meeting of the WHO Advisory ... "Status of CMX001 for Smallpox and Other dsDNA Viruses." ...
    Cached Biology Technology:Dendreon Announces MEDCAC Panel Reviewed PROVENGE Evidence 2Dendreon Announces MEDCAC Panel Reviewed PROVENGE Evidence 3Terso Solutions RFID Jump Start Takes the Risk Out of Implementing RFID for Hospitals and Research Laboratories Looking to Streamline Operations and Improve Regulatory Compliance 2Chimerix Presents Update on CMX001 Development to the World Health Organization Advisory Committee on Variola Virus Research 2Chimerix Presents Update on CMX001 Development to the World Health Organization Advisory Committee on Variola Virus Research 3
    (Date:8/20/2014)... this image of the Para and Mato Grosso states ... intentionally set in order to deforest the land. Deforestation ... trees where the land is thereafter converted to a ... to farms, ranches, or urban use. The herringbone-patterned tan ... Rainforest in the middle of the image are evidence ...
    (Date:8/20/2014)... Oklahoma Medical Research Foundation a five-year, $14.5 million grant ... effects on humans. , For 10 years, OMRF ... the human immune response to anthrax bacteria as part ... funding came soon after anthrax-laced letters killed five and ... of Sept. 11, 2001. , The long-term goal ...
    (Date:8/20/2014)... aroma of sunscreen and seawater signals a relaxing trip ... the idyllic beach vacation comes with an environmental hitch. ... the sea, they can become toxic to some of ... for many other marine animals. Their study appears in ... , Antonio Tovar-Sanchez and David Snchez-Quiles point out that ...
    Breaking Biology News(10 mins):$14.5 million grant awarded to continue anthrax studies 2
    ... may be one step closer to a ,recipe, for large-scale ... Induced pluripotent stem cells (iPSCs) can be of great ... many different types of cells. However, producing these cells is ... unknown. But researchers from the University of Toronto, the ...
    ... today in the journal Gondwana Research , has ... Indian tectonic plates, demonstrating how they formed the supercontinent ... Holloway University, The Australian National University and Geoscience Australia, ... plates evolved and where they should be positioned when ...
    ... that crabs may have disappeared from Antarctica only to return ... ago following the discovery of a major colony of King ... continental shelf off the Antarctic Peninsula. It was thought ... 15 million years ago and was returning as seawater temperatures ...
    Cached Biology News:Toronto team ID proteins key in stem cell production 2Evidence suggests Antarctic crabs could be native 2
    ... ( Abpromise for all tested applications). ... to amino acids 14/26 of Mouse Green cones ... acids 18/30 of tiger red cones opsin 1 ... Entrez Gene ID: 5956 ...
    Goat polyclonal to p27 KIP 1 ( Abpromise for all tested applications). Antigen: Synthetic peptide: C-KRPATDDS STQNK, corresponding to Internal sequence amino acids 153-165 of Human p27 KIP 1 ...
    Request Info...
    Myt 1 (N-17)...
    Biology Products: