Clearly, state-of-the-art proteomic technology, in combination with immunofluorescence analysis of cryostat sections, offers a powerful approach for searching for protein biomarkers that may be associated with the various stages of tumor progression. The heterogeneity of the tissues and tumors poses obvious restrictions in the interpretation of the proteome data, although we believe these limitations will be overcome as additional markers become available that define the various stages of tumor progression. Currently, a great deal of effort is being devoted to quantitate the tumor protein profiles using the phosphor imager marketed by Bio-Rad, as it may be possible to rely on quantitative expression patterns to characterize particular stages in tumor development.
We would like to thank I Andersen, B Basse, A Celis, JB Lauridsen, G Ratz, and B Hein for expert assistance and I Gromova and P Gromov for stimulating discussions. The work is being supported by grants from the Danish Cancer Society and the Biotechnology program.
Anderson L and Seilhamer J, A comparison of selected mRNA and protein abundances in human liver, Electrophoresis 18, 533537 (1997)
Celis JE et al., Comprehensive computerized 2D gel protein databases offer a global approach to the study of the mammalian cell, Bioassays 12, 9397 (1990)
Celis JE et al., The human keratinocyte two-dimensional gel protein database (update 1995): Mapping components of signal transduction pathways, Electrophoresis 16, 21772240 (1995)
Celis JE et al., Human 2-D PAGE databases for proteome ana