Information gathered on any given polypeptide, known or unknown, can be easily retrieved by clicking on the corresponding spot. A file containing all of the information entered for this particular protein, mostly obtained from experiments performed in our laboratory, appears as shown in Figure 2 (only a fraction of the file is shown). Files for known proteins contain links to public protein databases including a subset of MedLine, SWISS-PROT, and PDB. Other links include OMIM, GeneCards, Unigene, and other web sites such as CySPID, metabolic pathways, the cytokine explorer, histology images, etc. In the future, as new databases and related web sites become available, it will be possible to navigate through various databanks containing complementary information (nucleic acid and protein sequence, genome mapping, diseases, protein structure, posttranslational modifications, antibodies, signaling pathways, etc.). In particular, as the Human Genome Project progresses, 2-D PAGE databases are expected to annotate DNA sequences, and will be instrumental in linking protein and DNA sequencing
with mapping information, offering a global approach to the study of cell regulation (Celis et al. 1990, 1996a). In addition, human proteomic databanks are expected to expedite drug discovery by pinpointing candidate drug targets on the basis of changes in the proteome expression profile of biopsies obtained from patients and controls (Celis et al. 1996a).
Currently, a great deal of effort is being invested in the development
of similar 2-D PAGE databases of SCCs, TCCs, and urine (http://biobase.dk/cgi-bin/celis).