Navigation Links
Proteinase K, Recombinant, PCR Grade: the Ideal Tool for Template Preparations

The endopeptidase originally isolated from the filtrate of the lower fungus Tritirachium album limber is recombinantly expressed in Pichia pastoris. Proteinase K is extremely effective on native proteins from different sources, and remains active in the presence of denaturing reagents. This makes it a useful tool in the isolation of DNA/RNA species. The proteinase is an enzyme with a molecular weight of 28.9 kDa, as shown by MALDI mass spectroscopy, and consists of a single peptide chain of 279 amino acids. Like other serine proteases, the enzyme is inactivated by sulfonyl fluoride analogs such as Pefabloc SC*. The pH activity of Proteinase K for hydrolysis of the synthetic substrate Chromozym TRY* is optimal in the range pH 6.59.5.

For the isolation of undamaged high molecular-weight nucleic acids from different sources an increased purity of the product is attained. The most stringent specification parameters and the minimized contamination result in the best quality available on the market. That means absence of endonucleases, nicking activity, and ribonucleases, a DNA content of less than 10 pg/mg, and a low bioburden content of less than 5 cfu/ml in solution, and less than 125 cfu/g in the lyophilized form.

Advantages of the new recombinant Proteinase K

  • Improved overall performance due to lot-to-lot consistency
  • Superior quality due to a minimized contaminant level
  • Reliable and sensitive tests are conducted to assay for trace contaminants, resulting in superior product purity

The performance of the new recombinant enzyme has been extensively tested and compared to the performance of the native enzyme (Figure 1). There are no differences in the performance or physical and chemical parameters compared to the native enzyme, thus no protocol changes are necessary.


'"/>

Source:


Page: All 1 2

Related biology technology :

1. pfuturbo DNA Polymerase: A High-Performance, High-Fidelity Enzyme Ideal for PCR Cloning
2. TripleMaster PCR SystemThe Ideal Choice for Long-Range PCR
3. TripleMaster and Perfect gDNA Blood Mini Kit Team Up to Amplify Long gDNA Templates
4. Using the TripleMaster PCR System for Robust Amplification of GC-Rich DNA Templates
5. Expand Long Template PCR System
6. High Pure PCR Template Preparation Kit
7. InstaGene Matrix: Prepare DNA Templates for PCR With No Phenol/Chloroform Extractions and No Deproteinization Steps, Rev C
8. Preparation of PCR Quality Template DNA from a Wide Range of Tissues Using InstaGene Matrix
9. Inactivation of Contaminating Templates in PCR Using High Intensity Ultraviolet Light
10. Scalable Automation of the TempliPhi DNA Sequencing Template Amplification and DYEnamic ET Terminator Sequencing Kits on the Sciclone ALH 3000
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:10/12/2017)... ... October 12, 2017 , ... ... multicenter, prospective clinical study that demonstrates the accuracy of the FebriDx® test, ... clinically significant acute bacterial and viral respiratory tract infections by testing the ...
(Date:10/12/2017)... ... October 12, 2017 , ... ... analysis platform specifically designed for life science researchers to analyze and interpret ... Rosalind Franklin, who made a major contribution to the discovery of the ...
(Date:10/11/2017)... ... ... is a basic first aid supply for any work environment, but most personal eye wash ... if a dangerous substance enters both eyes? It’s one less decision, and likely quicker response ... piece. , “Whether its dirt and debris, or an acid or alkali, getting anything in ...
(Date:10/11/2017)... 2017  VMS BioMarketing, a leading provider of patient support ... Nurse Educator (CNE) network, which will launch this week. The ... health care professionals to enhance the patient care experience by ... other health care professionals to help women who have been ... ...
Breaking Biology Technology:
(Date:8/23/2017)... N.Y. , Aug. 23, 2017  The general public,s help is ... human microbiome—the bacteria that live in and on the human body –and ... The ... in the human microbiome, starting with the gut. The project's goal is ... disease. Photo credit: IBM ...
(Date:6/23/2017)... ITHACA, N.Y. , June 23, 2017 /PRNewswire/ ... leader in dairy research, today announced a new collaboration ... reduce the chances that the global milk supply is ... dairy project, Cornell University has become the newest academic ... Supply Chain, a food safety initiative that includes IBM ...
(Date:5/16/2017)... , May 16, 2017   Bridge Patient ... organizations, and MD EMR Systems , an ... partner for GE, have established a partnership to ... product and the GE Centricity™ products, including Centricity ... These new integrations will allow ...
Breaking Biology News(10 mins):