Navigation Links
Proteinase K, Recombinant, PCR Grade: the Ideal Tool for Template Preparations

The endopeptidase originally isolated from the filtrate of the lower fungus Tritirachium album limber is recombinantly expressed in Pichia pastoris. Proteinase K is extremely effective on native proteins from different sources, and remains active in the presence of denaturing reagents. This makes it a useful tool in the isolation of DNA/RNA species. The proteinase is an enzyme with a molecular weight of 28.9 kDa, as shown by MALDI mass spectroscopy, and consists of a single peptide chain of 279 amino acids. Like other serine proteases, the enzyme is inactivated by sulfonyl fluoride analogs such as Pefabloc SC*. The pH activity of Proteinase K for hydrolysis of the synthetic substrate Chromozym TRY* is optimal in the range pH 6.59.5.

For the isolation of undamaged high molecular-weight nucleic acids from different sources an increased purity of the product is attained. The most stringent specification parameters and the minimized contamination result in the best quality available on the market. That means absence of endonucleases, nicking activity, and ribonucleases, a DNA content of less than 10 pg/mg, and a low bioburden content of less than 5 cfu/ml in solution, and less than 125 cfu/g in the lyophilized form.

Advantages of the new recombinant Proteinase K

  • Improved overall performance due to lot-to-lot consistency
  • Superior quality due to a minimized contaminant level
  • Reliable and sensitive tests are conducted to assay for trace contaminants, resulting in superior product purity

The performance of the new recombinant enzyme has been extensively tested and compared to the performance of the native enzyme (Figure 1). There are no differences in the performance or physical and chemical parameters compared to the native enzyme, thus no protocol changes are necessary.


'"/>

Source:


Page: All 1 2

Related biology technology :

1. pfuturbo DNA Polymerase: A High-Performance, High-Fidelity Enzyme Ideal for PCR Cloning
2. TripleMaster PCR SystemThe Ideal Choice for Long-Range PCR
3. TripleMaster and Perfect gDNA Blood Mini Kit Team Up to Amplify Long gDNA Templates
4. Using the TripleMaster PCR System for Robust Amplification of GC-Rich DNA Templates
5. Expand Long Template PCR System
6. High Pure PCR Template Preparation Kit
7. InstaGene Matrix: Prepare DNA Templates for PCR With No Phenol/Chloroform Extractions and No Deproteinization Steps, Rev C
8. Preparation of PCR Quality Template DNA from a Wide Range of Tissues Using InstaGene Matrix
9. Inactivation of Contaminating Templates in PCR Using High Intensity Ultraviolet Light
10. Scalable Automation of the TempliPhi DNA Sequencing Template Amplification and DYEnamic ET Terminator Sequencing Kits on the Sciclone ALH 3000
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:6/23/2016)... , June 23, 2016  Blueprint Bio, a company ... to the medical community, has closed its Series A ... Nunez . "We have received a commitment ... capital we need to meet our current goals," stated ... us the runway to complete validation on the current ...
(Date:6/23/2016)... Francisco, CA (PRWEB) , ... June 23, 2016 ... ... (EDC) software, is exhibiting at the Pennsylvania Convention Center and will showcase its ... Annual conference. ClinCapture will also be presenting a scientific poster on Disrupting Clinical ...
(Date:6/23/2016)... , June 22, 2016  Amgen (NASDAQ: ... of the QB3@953 life sciences incubator to ... health. The shared laboratory space at QB3@953 was created ... a key obstacle for many early stage organizations - ... of the sponsorship, Amgen launched two "Amgen Golden Ticket" ...
(Date:6/22/2016)... 22, 2016 Research and Markets has announced ... report to their offering. ... from $29.3 billion in 2013. The market is expected to grow ... 2015 to 2020, increasing from $50.6 billion in 2015 to $96.6 ... during the forecast period (2015 to 2020) are discussed. As well, ...
Breaking Biology Technology:
(Date:5/20/2016)... , May 20, 2016  VoiceIt is ... partnership with VoicePass. By working together, ... experience.  Because VoiceIt and VoicePass take slightly different ... engines increases both security and usability. ... excitement about this new partnership. "This ...
(Date:5/12/2016)... 2016 WearablesResearch.com , a brand of ... results from the Q1 wave of its quarterly wearables ... consumers, receptivity to a program where they would receive ... insurance company. "We were surprised to see ... Michael LaColla , CEO of Troubadour Research, "primarily because ...
(Date:5/3/2016)...  Neurotechnology, a provider of high-precision biometric identification ... Identification System (ABIS) , a complete system for ... can process multiple complex biometric transactions with high ... face or iris biometrics. It leverages the core ... MegaMatcher Accelerator , which have been used in ...
Breaking Biology News(10 mins):