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Protein Depletion for Plasma and Serum Proteomic Analysis

Human plasma and serum represents an important biological material for disease diagnosis. However, the wide dynamic range in protein concentration remains a major challenge in the development of diagnostic assays for the very low concentration of biomarker proteins in the presence of high abundance proteins. A practical and effective strategy is to remove 99% of the diagnostically uninformative proteins in order to enhance the detection of the low abundance proteins and penetrate deeper into the plasma proteome. Among a number of plasma protein depletion techniques, the ProteoPrep 20 represents the most powerful enabling technology currently available.


1. INTRODUCTION
1.1 Why blood plasma?
Blood plasma is not only the most studied among biological fluids, but also the primary material for disease diagnosis. Blood plasma contains a very high concentration of proteins, typically in the range of 60-80 mg of protein per ml. Estimates of the number of proteins in blood plasma start from 10,000, but the actual number of distinct proteins may be several orders of magnitude higher [1,2]. This is because each protein has a potential for a variety of post-translational and metabolic modifications [3-6], both in normal and diseased cells.

The global composition of proteins in the blood plasma represents the plasma proteome. Perfusion of blood through the different organs and tissues can result in the addition of new proteins, removal of some proteins, or modification of existing proteins, which may vary according to specific physiological or pathological conditions [7-14]. It is logical to expect correlation between the proteomic profiles of
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