Navigation Links
Protein Depletion for Plasma and Serum Proteomic Analysis

Human plasma and serum represents an important biological material for disease diagnosis. However, the wide dynamic range in protein concentration remains a major challenge in the development of diagnostic assays for the very low concentration of biomarker proteins in the presence of high abundance proteins. A practical and effective strategy is to remove 99% of the diagnostically uninformative proteins in order to enhance the detection of the low abundance proteins and penetrate deeper into the plasma proteome. Among a number of plasma protein depletion techniques, the ProteoPrep 20 represents the most powerful enabling technology currently available.


1. INTRODUCTION
1.1 Why blood plasma?
Blood plasma is not only the most studied among biological fluids, but also the primary material for disease diagnosis. Blood plasma contains a very high concentration of proteins, typically in the range of 60-80 mg of protein per ml. Estimates of the number of proteins in blood plasma start from 10,000, but the actual number of distinct proteins may be several orders of magnitude higher [1,2]. This is because each protein has a potential for a variety of post-translational and metabolic modifications [3-6], both in normal and diseased cells.

The global composition of proteins in the blood plasma represents the plasma proteome. Perfusion of blood through the different organs and tissues can result in the addition of new proteins, removal of some proteins, or modification of existing proteins, which may vary according to specific physiological or pathological conditions [7-14]. It is logical to expect correlation between the proteomic profiles of
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32

Related biology technology :

1. A New C-Terminal GST Vector for Protein Production in S. pombe
2. New Mammalian Two-Hybrid System Detects Protein-Protein Interactions
3. Antibodies for Studying NMDA Receptor Protein Expression and Synapse-Specific Immunolabeling
4. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
5. New Yeast Cloning System for Producing Proteins with Native Amino Acid Sequences
6. Optimized Imaging of Protein Gels Stained with Coomassie Brilliant Blue Dye
7. High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli
8. Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
9. Yeast Protein Production System Features High Yields and One-Step Purification
10. Efficient Cleavage of Fusion Proteins to Yield Native Amino Termini
11. Expression and Purification of Recombinant Proteins That Have Native Amino Acid Sequence
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Protein Depletion for Plasma and Serum Proteomic Analysis

(Date:8/29/2014)... Aug. 29, 2014 /PRNewswire/ - iCo Therapeutics ("iCo" ... reported financial results for the six months ended ... expressed in Canadian dollars and presented under International ... 2014 Highlights , Announced top-line results ... primary endpoint for subjects enrolled in the Phase ...
(Date:8/29/2014)... (PRWEB) August 29, 2014 A major ... the biotech start-up dense realm of Boston-Cambridge, is gaining ... able investors. James Sherley, the Director of Boston’s Adult ... the local and national visibility of his company an ... 2013. , In addition to a social media marketing ...
(Date:8/28/2014)... Ca. (PRWEB) August 28, 2014 Best ... rating for an alcohol-based hand sanitizer, asks food processors ... by comparing the hand sanitizer they’re currently using to ... . Hand hygiene is critical to fighting cross-contamination and ... Best Sanitizers believes there are key criteria that ...
(Date:8/28/2014)... Aug. 28, 2014 ... market research report is available ... Whole Exome Sequencing Market by ... Target Enrichment), by Services (Sequencing), ... by Application (Cancer, Monogenic disorders) ...
Breaking Biology Technology:iCo Therapeutics Announces Second Quarter 2014 Financial Results 2iCo Therapeutics Announces Second Quarter 2014 Financial Results 3iCo Therapeutics Announces Second Quarter 2014 Financial Results 4The Adult Stem Cell Technology Center, LLC Participates in Multiple Stem Cell and Regenerative Medicine Conferences Before Year End 2The Adult Stem Cell Technology Center, LLC Participates in Multiple Stem Cell and Regenerative Medicine Conferences Before Year End 3The Adult Stem Cell Technology Center, LLC Participates in Multiple Stem Cell and Regenerative Medicine Conferences Before Year End 4Food Processors and Food Handlers are Encouraged to Prepare for Fall Harvest by Evaluating Their Current Hand Sanitizer Using Nine Key Criteria 2Food Processors and Food Handlers are Encouraged to Prepare for Fall Harvest by Evaluating Their Current Hand Sanitizer Using Nine Key Criteria 3Whole Exome Sequencing Market by Product (Systems, Kits (Library Preparation, Target Enrichment), by Services (Sequencing), by Technology (Sequencing by Synthesis), by Application (Cancer, Monogenic disorders) - Global Forecast to 2018 2Whole Exome Sequencing Market by Product (Systems, Kits (Library Preparation, Target Enrichment), by Services (Sequencing), by Technology (Sequencing by Synthesis), by Application (Cancer, Monogenic disorders) - Global Forecast to 2018 3Whole Exome Sequencing Market by Product (Systems, Kits (Library Preparation, Target Enrichment), by Services (Sequencing), by Technology (Sequencing by Synthesis), by Application (Cancer, Monogenic disorders) - Global Forecast to 2018 4Whole Exome Sequencing Market by Product (Systems, Kits (Library Preparation, Target Enrichment), by Services (Sequencing), by Technology (Sequencing by Synthesis), by Application (Cancer, Monogenic disorders) - Global Forecast to 2018 5Whole Exome Sequencing Market by Product (Systems, Kits (Library Preparation, Target Enrichment), by Services (Sequencing), by Technology (Sequencing by Synthesis), by Application (Cancer, Monogenic disorders) - Global Forecast to 2018 6Whole Exome Sequencing Market by Product (Systems, Kits (Library Preparation, Target Enrichment), by Services (Sequencing), by Technology (Sequencing by Synthesis), by Application (Cancer, Monogenic disorders) - Global Forecast to 2018 7Whole Exome Sequencing Market by Product (Systems, Kits (Library Preparation, Target Enrichment), by Services (Sequencing), by Technology (Sequencing by Synthesis), by Application (Cancer, Monogenic disorders) - Global Forecast to 2018 8Whole Exome Sequencing Market by Product (Systems, Kits (Library Preparation, Target Enrichment), by Services (Sequencing), by Technology (Sequencing by Synthesis), by Application (Cancer, Monogenic disorders) - Global Forecast to 2018 9Whole Exome Sequencing Market by Product (Systems, Kits (Library Preparation, Target Enrichment), by Services (Sequencing), by Technology (Sequencing by Synthesis), by Application (Cancer, Monogenic disorders) - Global Forecast to 2018 10Whole Exome Sequencing Market by Product (Systems, Kits (Library Preparation, Target Enrichment), by Services (Sequencing), by Technology (Sequencing by Synthesis), by Application (Cancer, Monogenic disorders) - Global Forecast to 2018 11Whole Exome Sequencing Market by Product (Systems, Kits (Library Preparation, Target Enrichment), by Services (Sequencing), by Technology (Sequencing by Synthesis), by Application (Cancer, Monogenic disorders) - Global Forecast to 2018 12Whole Exome Sequencing Market by Product (Systems, Kits (Library Preparation, Target Enrichment), by Services (Sequencing), by Technology (Sequencing by Synthesis), by Application (Cancer, Monogenic disorders) - Global Forecast to 2018 13
... Dec. 3 Thermo Fisher,Scientific Inc. (NYSE: TMO ... the China Doping Control Center (CDCC) has selected the,company ... software for,the testing of athletes at the 29th Olympic ... Chinese government and the International Olympic,Committee (IOC) to conduct ...
... Dec. 3 Signalife, Inc.,(Amex: SGN ) ... Congress on,Cardiac Pacing and Electrophysiology to be held ... Dr. Muricio Arruda, who has been testing,Signalife,s wireless ... the Cleveland Clinic Heart Center, will,present a prototoype ...
... IDenta Corp.,(OTC: IDTA.PK - News) announced today that it ... kit that will detect the presence of lead in ... outbreak of lead,contamination in products produced in China prompted ... unnamed, the new IDenta lead detector will be,targeted for ...
Cached Biology Technology:Thermo Fisher Scientific Selected by China Doping Control Center to Supply Testing Instrumentation for the 2008 Olympic Games in Beijing 2Thermo Fisher Scientific Selected by China Doping Control Center to Supply Testing Instrumentation for the 2008 Olympic Games in Beijing 3Thermo Fisher Scientific Selected by China Doping Control Center to Supply Testing Instrumentation for the 2008 Olympic Games in Beijing 4Signalife Presence at XIII World Congress on Cardiac Pacing and Electrophysiology 2Signalife Presence at XIII World Congress on Cardiac Pacing and Electrophysiology 3International Substance Detection Firm IDenta To Release New Lead Test Kit - Rash Of Toy Incidents Leads Company To Enter New Marketplace 2International Substance Detection Firm IDenta To Release New Lead Test Kit - Rash Of Toy Incidents Leads Company To Enter New Marketplace 3
(Date:8/29/2014)... Until recently, little has been known about what ... international team of scientists, one of whom is a ... by showing that genes controlling the development of the ... rabbit domestication., The study was published Aug. 28 in ... questions. It is online at http://www.sciencemag.org/ ., The ...
(Date:8/29/2014)... the repeated climatic shifts over the last 120,000 ... diversity, a team of researchers led by City ... developed a new biodiversity metric called "phylogeographic endemism." ... genetic variation within species is restricted in geographical ... biology, and 14 other researchers from institutions in ...
(Date:8/29/2014)... This news release is available in German . ... are key players in the natural nitrogen cycle on ... these specialist bacteria were thought to depend on nitrite ... scientists led by Holger Daims, a microbiologist at the ... can use hydrogen as an alternative source of energy. ...
Breaking Biology News(10 mins):New research reveals how wild rabbits were genetically transformed into tame rabbits 2New research reveals how wild rabbits were genetically transformed into tame rabbits 3CCNY team defines new biodiversity metric 2Hydrogen powers important nitrogen-transforming bacteria 2Hydrogen powers important nitrogen-transforming bacteria 3
... at the University of Southampton have created a new method ... repair therapies for people with bone fractures or those who ... The research, carried out by Dr Emmajayne Kingham at the ... and published in the journal Small , cultured human ...
... DCA team of scientists, led by researchers at Carnegie,s ... widespread die-off of Colorado trembling aspen trees is a ... temperatures. The die-off, triggered by the drought from 2000-2003, ... Colorado aspen forests. In 2002, the drought subjected the ...
... temperatures and NMR spectroscopy, the scientists visualized seven intermediate ... from 25C to -16C. Their results show that the ... protein folding. The scientists, findings may contribute to a ... misfold during illness. Whether Alzheimer,s, Parkinson,s or ...
Cached Biology News:Stem cell breakthrough could lead to new bone repair therapies on nanoscale surfaces 2Tree die-off triggered by hotter temperatures 2Tree die-off triggered by hotter temperatures 3Protein 'filmed' while unfolding at atomic resolution 2Protein 'filmed' while unfolding at atomic resolution 3
... single use spatulas have been designed to ... are for those interested in eliminating the ... Each spatula is individually packaged, RNase/DNase free, ... is a new product number, created to ...
... of cells • Blade design minimizes cell ... growth surface • Designed for use in ... • Sterilized by gamma radiation and certified ... new product number, created to easily match ...
... Corning single use spatulas have been ... samples. They are for those interested in ... reusable spatulas. Each spatula is individually packaged, ... CLS number is a new product number, ...
... The MagneGST™ Protein Purification System provides ... for the purification of glutathione-S-transferase (GST) ... (MagneGST™ Particles) are used to isolate ... or cleared lysate using either a ...
Biology Products: