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Protector RNase Inhibitor Enhance the Protection of,,,RNA against Degradation

Protector RNase Inhibitor

  • Is a new type of RNase inhibitor and inhibits a wide known spectrum of RNases.
  • Does not interfere with the performance of enzymes in an assay - even when high amounts (e.g., up to 16-fold the standard concentration) of Protector RNase Inhibitor are needed to protect difficult RNA samples.
  • Is fully active over a wide pH range of 5.0 9.0.
  • Is fully active over a wide temperature range of 25C 55C. Even at 60C, some activity of the RNase Inhibitor is still measured.
Choose the highest measure of safety to avoid degradation of your RNA samples

Apply Protector RNase Inhibitor and improve your results in
  • Isolation and purification of RNA
  • RT-PCR reactions with conventional thermal cyclers and qPCR systems
  • cDNA synthesis reactions (e.g., when preparing targets for use on microarrays)
  • In vitro transcription/translation reactions
  • RNase protection assays
  • RNA-dependent in vitro functional assays
and all other applications in which RNases can destroy your precious RNA samples.

Why take unnecessary risks ?

Include Protector RNase Inhibitor in every assay.
Protector RNase Inhibitor inhibits a wide spectrum of different RNases (Table 1); up to 1 ng of RNase A is inhibited by Protector RNase Inhibitor (Figure).

Choose an RNase Inhibitor that can be used over a wide pH and temperature spectrum

Protector RNase Inhibitor is fully active over

  • A wide pH range o f 5.0 9.0 allowing its use in many different applications.
  • Fully active over a broad temperature range of 25C 55C. Even at 60C, some RNase inhibition is still measured. This is advantageous when performing reverse transcription reactions at elevated temperatures to overcome secondary structure in RNA (e.g., in combination with AMV Reverse Transcriptase to generate full length transcripts).

Choose an RNase Inhibitor that wont interfere with your established assays
Add Protector RNase Inhibitor to your reaction mixes without interfering with the performance of the other enzymes or the final result. Protector RNase Inhibitor is

  • Produced under GMP conditions and delivered according to specifications as a highly purified product without detectable endonucleases or nicking activity. In addition, the product has a DNA content of < 100 pg/mg and a bioburden of < 50 cfu/ml.
  • Completely free of detectable RNases (many commercially available inhibitors can themselves be contaminated).
  • Function tested in very demanding assays with the Titan RT-PCR System and the LightCycler Instrument.
  • Designed to inhibit RNases without interfering with enzymes or enzyme blends that are commonly used to analyze RNA (Table 2).


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Related biology technology :

1. Protector RNase Inhibitor
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3. Inducing RNAi with siRNA Cocktails Generated by RNase III
4. Dicer vs. RNase III for Preparation of siRNA Cocktails
5. Combat RNase Contamination in the Lab
6. Measuring RNase Activity A Real-time Kinetic Analysis
7. RNase Activity in Mouse Tissue: Classification, Hierarchy, and Methods for Control
8. A simple and rapid LC-MS/MS method for the simultaneous determination of nine antiretroviral drugs commonly used in Europe (protease Inhibitors and non-nucleoside reverse transcriptase inhibitors)
9. A Microtiter-based Assay for the Determination of ID50s of b-lactamase Inhibitors Employing Reporter Substrates Detected at UV or Visible Wavelengths (MaxLine Application Note #20)
10. Enhanced PCR Cloning System
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