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Protect RNA and Improve Target Cell Identification

1) and used for subsequent microarray analysis. Agilent bioanalyzer results show that yield (mean SD, 142 12 g) and size distribution (median, 1100 nt) of the aRNA were excellent in this experiment (Figure 5B).

Figure 5. Representative Results from qRT-PCR and aRNA Amplification Experiments from LCM Samples. (A) One-step qRT-PCR reaction to amplify HPRT mRNA was performed with total RNA isolated from human ovary LCM sample after staining with Cresyl Violet (see Figure 2; LCM Staining Kit, Ambion). (B) The MessageAmp II aRNA Kit (Ambion) was used to amplify 20 ng of total RNA isolated from mouse hippocampus and cerebellum after staining with Acridine Orange (AO) (see Figure 3) and Cresyl Violet (CV) (LCM Staining Kit, Ambion).


LCM Staining Kit Components

Each kit contains reagents sufficient for processing 80 slides with either stain. Additional components have been included in the kit that improve both staining quality and microdissection. For example, the kit comes with a barrier pen that is used to position the stains on the tissue sections; dehydration beads are provided to facilitate dehydration of the tissue--critical for successful LCM using the Arcturus instrument; RNase-free vessels hold the slides during processing; and RNaseZap and Nuclease-free Water minimize tissue sample exposure to environmental RNases. A CD that demonstrates several crucial
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