Navigation Links
Protect RNA and Improve Target Cell Identification

t or Acridine Orange in the LCM Staining Kit (Figure 4). Size profiles also indicate less RNA degradation after staining samples with the LCM Staining Kit vs a competitor's staining kit. (Figure 4).

Figure 4 . Higher RNA Quality with Ambion's LCM Stains. RNA was isolated from consecutive tissue sections that were either unstained or stained with three different dyes (Ambion's Cresyl Violet and Acridine Orange, and a Competitor Stain). The RNAqueous Micro Kit (Ambion) was used to process samples that were stained with the LCM Staining Kit. The Competitor's RNA isolation kit and dyes were used to process separate samples. All of the resulting RNA preparations were analyzed on an Agilent 2100 bioanalyzer. (A) Ratio of 28S rRNA to 18S rRNA of RNA preparations from various types of tumor samples. (B) Representative Agilent bioanalyzer traces for RNA preparations from human colon cancer sections show less degradation in the RNA isolated using the LCM Staining and RNAqueous-Micro Kits (top) compared to RNA isolated with the competitor's reagents (bottom).


RNA from LCM samples stained with Cresyl Violet or Acridine Orange can be used to analyze gene expression with either qRT-PCR or array analyses (Figure 5). The RNAqueous-Micro Kit was used to isolate RNA from the LCM sample shown in Figure 2. RNA from HPRT was amplified from this sample by qRT-PCR (Figure 5A). In addition, the RNAqueous-Micro Kit was used to isolate RNA from the LCM sample shown in Figure 3. This RNA (20 ng) was linearly amplified using the MessageAmp II aRNA Amplification Kit (Cat# 175
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. RNeasy Protect Mini, Midi, and Maxi Kits
2. Protector RNase Inhibitor
3. Protector RNase Inhibitor Enhance the Protection of RNA against Degradation
4. RNase Protection Assay (RPA) Using DIG-Labeled RNA Probes
5. Nuclease Protection Assays: The Basics
6. The Best Enzyme for the Toughest PCR Challenges Improved with New Hot-Start Feature
7. Novel PCR Enhancing Factor Improves Performance of Pfu DNA Polymerase
8. Improved Sensitivity for Staining RNA with SYBR Gold Stain
9. Improve Lipid- or Calcium Phosphate-Mediated Transfection of Human Dermal Fibroblasts
10. Improved Vectors for PathDetect Trans-Reporting Systems
11. Improve Amplification Specificity with Hot Start PCR Enzyme
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Protect RNA and Improve Target Cell Identification

(Date:8/4/2015)... PORTLAND, Oregon , August 4, 2015 /PRNewswire/ ... published by Allied Market Research, titled, "World Cardiac ... global cardiac biomarker market would generate the revenue ... of 18.9% from 2015 to 2020. Cardiac Troponins ... throughout the forecast period due to high sensitivity ...
(Date:8/4/2015)... ... August 04, 2015 , ... ... diagnostic tests – today announced that Robert A. Gregg, PhD, has joined RPS ... than 30 years of relevant experience and remarkable achievements in the medical device ...
(Date:8/4/2015)... Aug. 4, 2015  Blueprint Medicines (NASDAQ: BPMC ... kinase drugs for genomically defined diseases, today announced that ... a company overview at the 2015 Wedbush PacGrow Healthcare ... EDT. A live webcast of the event ... the Blueprint Medicines website at www.blueprintmedicines.com . An ...
(Date:8/4/2015)... Francisco, CA (PRWEB) , ... August 04, 2015 , ... ... difference by inviting a diverse selection of entrepreneurs from across the United States to ... CEO Jessica Richman is honored to be chosen to be among the attendees. , ...
Breaking Biology Technology:Cardiac Biomarker Market is Expected to Reach $2,085.9 Million by 2020 2Cardiac Biomarker Market is Expected to Reach $2,085.9 Million by 2020 3Cardiac Biomarker Market is Expected to Reach $2,085.9 Million by 2020 4Cardiac Biomarker Market is Expected to Reach $2,085.9 Million by 2020 5RPS Diagnostics Welcomes Robert Gregg as Senior Vice President of Clinical and Regulatory Affairs 2RPS Diagnostics Welcomes Robert Gregg as Senior Vice President of Clinical and Regulatory Affairs 3White House Invites uBiome CEO Jessica Richman to First-Ever Startup Demo Day 2
... Electronic health record systems generally are perceived to have ... more clinics consider moving from paper charts to EHR ... barriers to entry. , ,The evolution of systems for ... robust data security has prompted more hospitals and ...
... - Just six weeks after taking the helm at ... to pursue opportunities in the private sector, the organization announced. ... has served as general counsel to WiCell's parent, the ... high-tech and biotechnology industries. , ,Donley, who joined WARF in ...
... An education research team at the University of Wisconsin-Madison ... to study the impact of digital media on youth ... investment will support 24 national studies of different aspects of ... , ,"What MacArthur is actually trying to do, with this ...
Cached Biology Technology:GE Healthcare launches hybrid electronic record 2Donley resigns as WiCell director 2UW gets $3M to explore educational gaming 2UW gets $3M to explore educational gaming 3
(Date:7/21/2015)... , July 21, 2015 Passwords have ... seen in the recent U.S. Office of Personnel ... to the infosec conundrum, but developers and end-users ... To address this dilemma, Biometrics-as-a-Service provider HYPR Corp. ... Tokenization SDK for third party integration. ...
(Date:7/9/2015)... , July 07, 2015 ... announced the addition of the "Biometrics for ... Forecasts 2015-2020" report to their offering. ... major contributor to this growth and the forecast ... billion in revenue for companies involved in delivering ...
(Date:7/8/2015)... DIEGO , July 8, 2015  Trovagene, ... cell-free molecular diagnostics, today announced the launch of ... the Company,s Precision Cancer Monitoring℠ (PCM) technology for ... receiving one or a combination of the novel ... Opdivo® (nivolumab), a PD-1 inhibitor. The 50-patient study ...
Breaking Biology News(10 mins):HYPR Corp. Launches Industry First Biometric Tokenization Platform 2HYPR Corp. Launches Industry First Biometric Tokenization Platform 3HYPR Corp. Launches Industry First Biometric Tokenization Platform 4Global Banking Biometrics Market 2015-2020 - Market & Technology Analysis, Adoption Strategies and Forecasts 2Global Banking Biometrics Market 2015-2020 - Market & Technology Analysis, Adoption Strategies and Forecasts 3Trovagene Initiates Study to Monitor Response to Immunotherapy in Melanoma Patients Using its Precision Cancer Monitoring Platform 2Trovagene Initiates Study to Monitor Response to Immunotherapy in Melanoma Patients Using its Precision Cancer Monitoring Platform 3Trovagene Initiates Study to Monitor Response to Immunotherapy in Melanoma Patients Using its Precision Cancer Monitoring Platform 4
... a form of breast cancer known as estrogen receptor positive ... the treatment and prevention of recurring breast cancer. Currently, ... dose is prescribed for every patient. New research at ... patient-specific genetic factors, lack of exposure to vitamin D during ...
... DNA code for a complex region of the human genome ... Fraser University, chart new territory in immunity research. , They ... how well they operate and, potentially, what they fight off, ... drugs, treatments and vaccinations are designed to treat whole populations ...
... the Bellvitge Biomedical Research Institute (IDIBELL) and the Catalan ... study of seven missense variants of the BRCA1 gene ... to the risk of suffering breast or ovarian cancer. ... PLoS One The classification of these three ...
Cached Biology News:Cold winters freezing out breast cancer treatment 2Scientists find ethnicity linked to antibodies 2Identified as responsible for breast and ovarian hereditary cancer 3 mutations at BRCA1 gene 2
...
UltraPure DNase/RNase-Free Distilled Water is designed for use in all molecular biology applications. It is 0.1-m membrane-filtered and tested for DNase and RNase activity....
...
Water Distilled (Gibco™) is membrane filtered and endotoxin screened....
Biology Products: