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Protect RNA and Improve Target Cell Identification

t or Acridine Orange in the LCM Staining Kit (Figure 4). Size profiles also indicate less RNA degradation after staining samples with the LCM Staining Kit vs a competitor's staining kit. (Figure 4).

Figure 4 . Higher RNA Quality with Ambion's LCM Stains. RNA was isolated from consecutive tissue sections that were either unstained or stained with three different dyes (Ambion's Cresyl Violet and Acridine Orange, and a Competitor Stain). The RNAqueous Micro Kit (Ambion) was used to process samples that were stained with the LCM Staining Kit. The Competitor's RNA isolation kit and dyes were used to process separate samples. All of the resulting RNA preparations were analyzed on an Agilent 2100 bioanalyzer. (A) Ratio of 28S rRNA to 18S rRNA of RNA preparations from various types of tumor samples. (B) Representative Agilent bioanalyzer traces for RNA preparations from human colon cancer sections show less degradation in the RNA isolated using the LCM Staining and RNAqueous-Micro Kits (top) compared to RNA isolated with the competitor's reagents (bottom).


RNA from LCM samples stained with Cresyl Violet or Acridine Orange can be used to analyze gene expression with either qRT-PCR or array analyses (Figure 5). The RNAqueous-Micro Kit was used to isolate RNA from the LCM sample shown in Figure 2. RNA from HPRT was amplified from this sample by qRT-PCR (Figure 5A). In addition, the RNAqueous-Micro Kit was used to isolate RNA from the LCM sample shown in Figure 3. This RNA (20 ng) was linearly amplified using the MessageAmp II aRNA Amplification Kit (Cat# 175
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