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Protect RNA and Improve Target Cell Identification

ed with Cresyl Violet in Ambion's LCM Staining Kit. These representative photographs show an 8 m section from human ovary (20X magnification) that was stained with Cresyl Violet and dissected with the Pixel IIe LCM System (Arcturus). LCM sample Cluster of ovarian adenocarcinoma cells.


Acridine Orange (Fluorescent Intercalating Stain)

Acridine Orange stain intercalates into nucleic acids to enhance visability of nuclei in clusters of cells (Figure 3). Nuclear-rich epithelial tissues, such as clusters of malignant cells, are easily identified with this dye. Isolated cells stained with Acridine Orange will fluoresce on the cap after microdissection, making it easy to assess whether the cells were effectively microdissected and retrieved. If tissue morphology is slightly damaged, Acridine Orange may also provide better contrast than Cresyl Violet.

Figure 3. LCM Sample Stained with Acridine Orange in Ambion's LCM Staining Kit. These representative photographs show a 10 m section from mouse hippocampus (4X magnification) that was stained with Acridine Orange and subsequently dissected with the Pixel IIe LCM System (Arcturus). LCM sample Hippocampus granular cell layer and dentate gyrus.


Quality RNA for Downstream Applications

The integrity of RNA isolated after staining samples with Ambion's unique dye formulation is superior to RNA isolated using a competitor's stain (Figure 4). RNA integrity was assessed on an Agilent 2100 bioanalyzer. As seen in the electropherogram trace, a good 28S/18S rRNA ratio is maintained with human tumor samples stained with Cresyl Viole
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