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Protect RNA and Improve Target Cell Identification

CT-embedded tissues. The LCM Staining Kit enables researchers to obtain high quality total RNA from small, homogenous samples particularly when used in combination with Ambion's RNAqueous-Micro RNA Isolation Kit, which now contains additional reagents and protocols for use with LCM samples (see Isolate High Quality Total RNA from LCM Samples Suitable for Microarray and qRT-PCR Analysis).


Superior Staining to Identify Cells of Interest

The LCM Staining Kit includes two stains that produce superior results compared to conventional protocols. These stains greatly facilitate target cell identification during LCM (Figure 1). Only Ambion's staining procedures avoid exposing tissue sections to aqueous solutions that reactivate endogenous RNase activity.

Figure 1. Obtain Better Resolution with Ambion's LCM Staining Kit Dyes. Consecutive sections from human small intestine (20X magnification) were stained according to manufacturers' instructions.


Cresyl Violet (Basic Nuclear Stain)

Cresyl Violet is a basic synthetic dye that binds acidic components such as RNA-rich ribosomes, nuclei, and nucleoli. This histological stain has been used to stain neurological tissues and is especially useful for allowing researchers to identify cellular morphology and tissue architecture (see Figure 2).

Figure 2. LCM Sample Stain
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