Navigation Links
Properties of Reverse Transcriptases

Enzyme Maximum size of template Temperature optimum Units needed for standard reaction Ion require-
ment
Rnase H activity Combined RT and DNA Pol. Incorporates DIG-11-dUTP Expand Re-
verse Trans-
criptased up to 14kb 42C (up to 50C) 50 Mg2+ - - yes AMV Reverse Transcriptase up to 12kb 42C (up to 60C) 40 Mg2+ ++ - yes M-MuLV Reverse Transcriptase up to 10kb 37C 40 Mg2+ + - yes C. therm Polymerase for RT in Two-Step RT-PCR up to 4kb 60-70C 6 Mg2+ - -e yes Tth DNA Polymerase up to 1kb 60-70C 4 Mn2+ - + yes a Number in parentheses indicates maximum temperature tolerated by enzyme. b Standard reaction performed as recommended in enzyme package insert. c For nonradioact ive labeling; also incorporates other modified nucleotides. d Not for sale in the U.S. e C. therm. Polymerase for Reverse Transcription in Two-Step RT-PCR does not survive incubation at 95C. For RT-PCR, use the polymerase together with a second, thermostable enzyme such as Taq DNA Polymerase or Expand High Fidelity PCR System.
'"/>

Source:


Page: All 1 2

Related biology technology :

1. Properties of PCR Enzymes
2. Reverse transcriptases suitable for one-step RT-PCRb
3. Reverse transcriptases suitable for two-step RT-PCRd
4. C. therm. Polymerase for Reverse Transcription in Two-Step RT-PCR
5. Transcriptor Reverse Transcriptase
6. Increase the Power and Sensitivity for Your cDNA Synthesis with the New Transcriptor Reverse Transcriptase
7. Use of Transcriptor Reverse Transcriptase in Microarray Analysis
8. Recombinant HIV Reverse Transcriptase
9. Novel Bonding Chemistry Imparts Enhanced Polar Selectivity to TSK-GEL ODS-100V Reversed Phase Columns
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:5/24/2017)... , May 23, 2017 As Ebola resurfaces in ... four deaths and 20 suspected cases now reported, a new ... PubMed database, showed a correlation between the 2014 and 2017 ... counts rose sharply in 2012-13, which preceded the 2014 outbreak. ... the Ebola gene Replikin counts in 2014-15, which again precedes ...
(Date:5/23/2017)... ... May 23, 2017 , ... A recent survey conducted by the Weed Science ... control weed in 12 categories of broadleaf crops, fruits and vegetables, while common lambsquarters ... U.S. and Canada participated in the 2016 survey, the second conducted by WSSA. ...
(Date:5/23/2017)... ... May 23, 2017 , ... ... its 20th anniversary, marking the occasion with a strong presence at Bio-IT World ... Reception and further extends an invitation to all attendees to view posters ...
(Date:5/23/2017)... USA (PRWEB) , ... May 22, 2017 , ... ... Optics and Photonics 2017 in San Diego, California, this August will feature ... solar fuels, and autonomous vehicles. , SPIE Optics and Photonics, the largest multidisciplinary ...
Breaking Biology Technology:
(Date:5/16/2017)... --  Bridge Patient Portal , an enterprise patient ... Systems , an electronic medical record solutions developer ... a partnership to build an interface between the ... products, including Centricity Practice Solution (CPS), Centricity Business ... integrations will allow healthcare delivery networks using GE ...
(Date:4/18/2017)... 2017  Socionext Inc., a global expert in SoC-based imaging and ... the M820, which features the company,s hybrid codec technology. A demonstration ... Probe, Inc., will be showcased during the upcoming Medtec Japan at ... the Las Vegas Convention Center April 24-27. ... Click here for an ...
(Date:4/11/2017)... , April 11, 2017 Crossmatch®, a ... authentication solutions, today announced that it has been ... Research Projects Activity (IARPA) to develop next-generation Presentation ... "Innovation has been a driving force ... program will allow us to innovate and develop ...
Breaking Biology News(10 mins):