Navigation Links
Properties of Reverse Transcriptases

Enzyme Maximum size of template Temperature optimum Units needed for standard reaction Ion require-
ment
Rnase H activity Combined RT and DNA Pol. Incorporates DIG-11-dUTP Expand Re-
verse Trans-
criptased up to 14kb 42C (up to 50C) 50 Mg2+ - - yes AMV Reverse Transcriptase up to 12kb 42C (up to 60C) 40 Mg2+ ++ - yes M-MuLV Reverse Transcriptase up to 10kb 37C 40 Mg2+ + - yes C. therm Polymerase for RT in Two-Step RT-PCR up to 4kb 60-70C 6 Mg2+ - -e yes Tth DNA Polymerase up to 1kb 60-70C 4 Mn2+ - + yes a Number in parentheses indicates maximum temperature tolerated by enzyme. b Standard reaction performed as recommended in enzyme package insert. c For nonradioact ive labeling; also incorporates other modified nucleotides. d Not for sale in the U.S. e C. therm. Polymerase for Reverse Transcription in Two-Step RT-PCR does not survive incubation at 95C. For RT-PCR, use the polymerase together with a second, thermostable enzyme such as Taq DNA Polymerase or Expand High Fidelity PCR System.
'"/>

Source:


Page: All 1 2

Related biology technology :

1. Properties of PCR Enzymes
2. Reverse transcriptases suitable for one-step RT-PCRb
3. Reverse transcriptases suitable for two-step RT-PCRd
4. C. therm. Polymerase for Reverse Transcription in Two-Step RT-PCR
5. Transcriptor Reverse Transcriptase
6. Increase the Power and Sensitivity for Your cDNA Synthesis with the New Transcriptor Reverse Transcriptase
7. Use of Transcriptor Reverse Transcriptase in Microarray Analysis
8. Recombinant HIV Reverse Transcriptase
9. Novel Bonding Chemistry Imparts Enhanced Polar Selectivity to TSK-GEL ODS-100V Reversed Phase Columns
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:9/21/2017)... ... September 21, 2017 , ... ... sector professionals, has announced the addition of 5 new courses to its prospectus. ... Compliance with Regulation 21 CFR Part 11 on Electronic Records and Electronic Signatures ...
(Date:9/21/2017)... ... September 21, 2017 , ... Lajollacooks4u welcomed the San Diego chapter of Les ... society of professional women with high achievement in the fields of food, fine beverage ... , Twelve members began with an olive oil tasting to whet their palettes and ...
(Date:9/20/2017)... , ... September 20, 2017 , ... ... collaboration with Koch Agronomic Services (Koch) to feature new innovations aimed at helping ... broadcast first quarter 2018. American Farmer airs Tuesdays at 8:30aET on RFD-TV. Check ...
(Date:9/20/2017)... ... ... Diversity focused business accelerator, The Refinery , announced today they are hosting ... technology-driven, women-led startups in Boston, MA, New Haven/Hamden, CT, and Newark, NJ. Fueling ... that week – in Boston, it will be part of the City of Boston's ...
Breaking Biology Technology:
(Date:4/13/2017)... MONICA, Calif. , April 13, 2017 ... New York will feature emerging and evolving ... Summits. Both Innovation Summits will run alongside the expo ... of speaker sessions, panels and demonstrations focused on trending ... coast,s largest advanced design and manufacturing event will take ...
(Date:4/11/2017)... April 11, 2017 No two people ... at the New York University Tandon School of ... have found that partial similarities between prints are ... in mobile phones and other electronic devices can ... The vulnerability lies in the fact that fingerprint-based ...
(Date:4/5/2017)... , April 5, 2017 Today HYPR ... that the server component of the HYPR platform is ... providing the end-to-end security architecture that empowers biometric authentication ... HYPR has already secured over 15 million users across ... manufacturers of connected home product suites and physical access ...
Breaking Biology News(10 mins):