Navigation Links
Properties of Reverse Transcriptases

Enzyme Maximum size of template Temperature optimum Units needed for standard reaction Ion require-
ment
Rnase H activity Combined RT and DNA Pol. Incorporates DIG-11-dUTP Expand Re-
verse Trans-
criptased up to 14kb 42C (up to 50C) 50 Mg2+ - - yes AMV Reverse Transcriptase up to 12kb 42C (up to 60C) 40 Mg2+ ++ - yes M-MuLV Reverse Transcriptase up to 10kb 37C 40 Mg2+ + - yes C. therm Polymerase for RT in Two-Step RT-PCR up to 4kb 60-70C 6 Mg2+ - -e yes Tth DNA Polymerase up to 1kb 60-70C 4 Mn2+ - + yes a Number in parentheses indicates maximum temperature tolerated by enzyme. b Standard reaction performed as recommended in enzyme package insert. c For nonradioact ive labeling; also incorporates other modified nucleotides. d Not for sale in the U.S. e C. therm. Polymerase for Reverse Transcription in Two-Step RT-PCR does not survive incubation at 95C. For RT-PCR, use the polymerase together with a second, thermostable enzyme such as Taq DNA Polymerase or Expand High Fidelity PCR System.
'"/>

Source:


Page: All 1 2

Related biology technology :

1. Properties of PCR Enzymes
2. Reverse transcriptases suitable for one-step RT-PCRb
3. Reverse transcriptases suitable for two-step RT-PCRd
4. C. therm. Polymerase for Reverse Transcription in Two-Step RT-PCR
5. Transcriptor Reverse Transcriptase
6. Increase the Power and Sensitivity for Your cDNA Synthesis with the New Transcriptor Reverse Transcriptase
7. Use of Transcriptor Reverse Transcriptase in Microarray Analysis
8. Recombinant HIV Reverse Transcriptase
9. Novel Bonding Chemistry Imparts Enhanced Polar Selectivity to TSK-GEL ODS-100V Reversed Phase Columns
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:10/12/2017)... ... 12, 2017 , ... They call it the “hairy ball.” ... depiction of a system of linkages and connections so complex and dense that ... computer science at Worcester Polytechnic Institute (WPI) and director of the university’s bioinformatics ...
(Date:10/12/2017)... ca (PRWEB) , ... October 12, 2017 , ... ... the Surgical Wound Market with the addition of its newest module, US Hemostats ... $1.2B market for thrombin hemostats, absorbable hemostats, fibrin sealants, synthetic sealants and biologic ...
(Date:10/11/2017)... ... October 11, 2017 , ... ... in its endogenous context, enabling overexpression experiments and avoiding the use of exogenous ... RNA guides is transformative for performing systematic gain-of-function studies. , This complement ...
(Date:10/11/2017)... , Oct. 11, 2017  VMS BioMarketing, a leading provider ... nationwide oncology Clinical Nurse Educator (CNE) network, which will launch ... for communication among health care professionals to enhance the patient ... office staff, and other health care professionals to help women ... cancer. ...
Breaking Biology Technology:
(Date:4/5/2017)... YORK , April 5, 2017 Today ... is announcing that the server component of the HYPR ... known for providing the end-to-end security architecture that empowers ... HYPR has already secured over 15 million ... makers including manufacturers of connected home product suites and ...
(Date:3/30/2017)... LOS ANGELES , March 30, 2017  On ... Hack the Genome hackathon at ... This exciting two-day competition will focus on developing health ... experience. Hack the Genome is ... has been tremendous. The world,s largest companies in the ...
(Date:3/28/2017)... The report "Video Surveillance Market ... Storage Devices), Software (Video Analytics, VMS), and Service (VSaaS, ... to 2022", published by MarketsandMarkets, the market was valued ... to reach USD 75.64 Billion by 2022, at a ... year considered for the study is 2016 and the ...
Breaking Biology News(10 mins):