Navigation Links
Properties of PCR Enzymes

The primary requirements for a DNA polymerase used in PCR are optimal activity at temperatures around 75C and the ability to retain that activity after prolonged incubation at even higher temperatures (95C). Initially, single DNA polymerases were used in PCR, where Taq DNA Polymerase was used for standard PCR, Tth DNA Polymerase for combined RT-PCR, and Pwo DNA Polymerase when fidelity of PCR was important. As researchers wanted to amplify longer templates, the enzyme blend concept was born, then optimized to produce enzyme blends that could amplify very long DNA (up to 35 kb targets from a genomic template; see Expand Long Template PCR System and Expand 20 kbPLUS PCR System). Finally, as the demand for more robust PCR, higher yield and fidelity increased, blends of two polymerases, such as the Expand High Fidelity PCR System, were optimized for various applications. These blends actually outperformed the individual DNA polymerases. To find the Roche polymerase product most suited for your application problem, please use our Product Selection Guide.
'"/>

Source:


Page: All 1

Related biology technology :

1. Properties of Reverse Transcriptases
2. Comparing Fidelity and Performance of Proofreading PCR Enzymes
3. Choice of RT-PCR Enzymes
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:10/11/2017)... ... , ... Disappearing forests and increased emissions are the main causes of the ... Especially those living in larger cities are affected by air pollution related diseases. , ... pollution-affected countries globally - decided to take action. , “I knew I had to ...
(Date:10/10/2017)... ... October 10, 2017 , ... ... (ADC) therapeutics, today confirmed licensing rights that give it exclusive global access ... developed in collaboration with Children’s Hospital Los Angeles (CHLA). Additionally, an ...
(Date:10/10/2017)... , Oct. 10, 2017 SomaGenics announced ... the NIH to develop RealSeq®-SC (Single Cell), expected to ... profiling small RNAs (including microRNAs) from single cells using ... highlights the need to accelerate development of approaches to ... "New techniques for measuring levels of ...
(Date:10/9/2017)... ... , ... The award-winning American Farmer television series will feature 3 Bar Biologics ... at 8:30aET on RFD-TV. , With global population estimates nearing ten billion people ... to feed a growing nation. At the same time, many of our valuable resources ...
Breaking Biology Technology:
(Date:4/19/2017)... New York , April 19, 2017 ... competitive, as its vendor landscape is marked by the ... the market is however held by five major players ... Safran. Together these companies accounted for nearly 61% of ... of the leading companies in the global military biometrics ...
(Date:4/11/2017)... , April 11, 2017 Crossmatch®, ... secure authentication solutions, today announced that it has ... Advanced Research Projects Activity (IARPA) to develop next-generation ... program. "Innovation has been a driving ... Thor program will allow us to innovate and ...
(Date:4/5/2017)... , April 5, 2017  The Allen Institute ... Allen Cell Explorer: a one-of-a-kind portal and dynamic digital ... 3D imaging data, the first application of deep learning ... human stem cell lines and a growing suite of ... platform for these and future publicly available resources created ...
Breaking Biology News(10 mins):