Navigation Links
Properties of PCR Enzymes

The primary requirements for a DNA polymerase used in PCR are optimal activity at temperatures around 75C and the ability to retain that activity after prolonged incubation at even higher temperatures (95C). Initially, single DNA polymerases were used in PCR, where Taq DNA Polymerase was used for standard PCR, Tth DNA Polymerase for combined RT-PCR, and Pwo DNA Polymerase when fidelity of PCR was important. As researchers wanted to amplify longer templates, the enzyme blend concept was born, then optimized to produce enzyme blends that could amplify very long DNA (up to 35 kb targets from a genomic template; see Expand Long Template PCR System and Expand 20 kbPLUS PCR System). Finally, as the demand for more robust PCR, higher yield and fidelity increased, blends of two polymerases, such as the Expand High Fidelity PCR System, were optimized for various applications. These blends actually outperformed the individual DNA polymerases. To find the Roche polymerase product most suited for your application problem, please use our Product Selection Guide.
'"/>

Source:


Page: All 1

Related biology technology :

1. Properties of Reverse Transcriptases
2. Comparing Fidelity and Performance of Proofreading PCR Enzymes
3. Choice of RT-PCR Enzymes
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:2/9/2016)... ... February 09, 2016 , ... ... President for Public Policy for the National Organization for Rare Disorders (NORD). Dorman ... to ensure their voices are heard throughout the drug regulatory review process. , ...
(Date:2/9/2016)... YORK , Feb. 9, 2016 This ... analyzes the current and future prospects of the market ... this report include companies engaged in the manufacture of ... an executive summary with a market snapshot providing the ... scope of this report. This section also provides the ...
(Date:2/9/2016)... Three-Year Initiative Supports Next Generation of Medical Geneticists ... Camp Experiences SHPG ... lives of children born with rare diseases, as well as the ... announcing a new initiative designed to positively affect the lives of ... rare disease care. --> To mark the company,s founding ...
(Date:2/9/2016)... 2016 DelveInsight,s, "Protein-Tyrosine ... provides in depth insights on the pipeline ... Protein-Tyrosine Phosphatase 1B (PTP1B) Inhibitors. The DelveInsight,s ... stages of development including Discovery, Pre-clinical, IND, ... Preregistration. Report covers the product clinical trials ...
Breaking Biology Technology:
(Date:1/21/2016)... 2016 --> ... research report "Emotion Detection and Recognition Market by Technology (Bio-Sensors, ... Expression, Voice Recognition and Others), Services, Application Areas, ... 2020", published by MarketsandMarkets, the global Emotion Detection ... 22.65 Billion by 2020, at a CAGR of ...
(Date:1/15/2016)... 2016 Recent publicized breaches in cyber security ... ways to ensure data security and user authentication in ... Android that ties a user,s mobile number ... a hardware authorization token. Customer service agents who employ ... their KodeKey enabled device to verify their identity.  Companies ...
(Date:1/8/2016)... MANCHESTER, United Kingdom , Jan. 8, 2016   ... diagnostic products, today announced the closing of a $9 million ... Proceeds from the financing will be used to accelerate the ... for detecting early-stage pressure ulcers. United ... receiving CE Mark approval. The device,s introduction has been met ...
Breaking Biology News(10 mins):