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Properties of PCR Enzymes

The primary requirements for a DNA polymerase used in PCR are optimal activity at temperatures around 75C and the ability to retain that activity after prolonged incubation at even higher temperatures (95C). Initially, single DNA polymerases were used in PCR, where Taq DNA Polymerase was used for standard PCR, Tth DNA Polymerase for combined RT-PCR, and Pwo DNA Polymerase when fidelity of PCR was important. As researchers wanted to amplify longer templates, the enzyme blend concept was born, then optimized to produce enzyme blends that could amplify very long DNA (up to 35 kb targets from a genomic template; see Expand Long Template PCR System and Expand 20 kbPLUS PCR System). Finally, as the demand for more robust PCR, higher yield and fidelity increased, blends of two polymerases, such as the Expand High Fidelity PCR System, were optimized for various applications. These blends actually outperformed the individual DNA polymerases. To find the Roche polymerase product most suited for your application problem, please use our Product Selection Guide.
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