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Prominence UFLC

r, it is necessary to decrease the sample injection volume in proportion to the column cross-sectional area (about 2 L to 5 L injection volume with 3 mm internal diameter column). With the Prominence UFLC, the SIL- 20AHT/20ACHT uses a high-performance measuring pump and enhanced air tightness of flow lines to achieve peak area repeatability (RSD) of 0.3% or less, even with injections of 2 L to 5 L.

Carryover has gained a great deal of attention in recent years, especially with todays sensitive triple quad mass spectrometers. For ultra-fast LC, reduced carryover is a very important factor, but combating sample carryover may increase analysis cycle time if additional autosampler needle rinsing is required. The Prominence UFLC SIL- 20AHT/20ACHT autosamplers successfully reduce carryover using the same proven techniques of the SIL- 20A/AC models. These include inhibiting the adsorption of samples to needle and seal surfaces, and an option for two different rinse solvents. Based on Shimadzu test parameters, carryover for the SIL-20AHT/10ACHT is typically 0.005% or less.

Figure 3 shows a chromatogram and reproducibility of retention time and peak area of 7 alkyl phenones under steep gradient conditions and a 1-minute run time. Prominence UFLC provided excellent reproducibility on all compounds with a 2l injection. When using instruments designed for high-pressure operation, it is conceivable that there may be problems with the durability of maintenance parts, ease of maintenance and quality of analysis. In the case of a high-pressure autosampler, the longevity of the high-pressure valve seal is shortened. Prominence UFLC uses Shimadzus own proprietary high-precision 6-port valve, which achieves 0.3% RSD or less area repeatability in 100,000 cycle endurance tests (based on Shimadzu test parameters) as shown in Table 1 . As shown, Prominence UFL
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