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Promegas Multiplexed Luciferase Reporter and Cell Viability Assays performed on the PHERAstar

ion note, we demonstrate the combination of several Promega cell-based assays multiplexed in both low-volume 384- and 1536-well plate formats. The BMG LABTECH PHERAstar microplate reader is used to record luminescence. Table 1 highlights the assays used in this application.


Materials and Methods

  • HEK293 cells stably transfected with a CRE/CL1 hPEST Renilla construct
  • Isoproterenol
  • Corning Low-volume 384-well plates, catalog no. 3673
  • Corning 1536-well plates, catalog no. 3937
  • Promega CellTiter-Glo Luminescent Cell Viability Assay, catalog no. G7570*
  • Promega EnduRen Live Cell Substrate, catalog no. E6482*
  • Promega ViviRen Live Cell Substrate, catalog no. E6492*
  • Deerac Fluidics Equator HTS low-volume liquid dispenser
  • BMG LABTECH PHERAstar (figure 1)
  • BMG LABTECH PHERAstar luminescence optic module

  • Multiplexing Luciferase Reporter and Cell Viability Assays
    Promegas luminescent CellTiter-Glo assay was multiplexed with either the luminescent EnduRen Live Cell Substrate, or the luminescent ViviRen Live Cell Substrate. The experimental set-up was similar for each assay combination.

    For the low-volume 384 assay format, a density of 12,000 stably transfected HEK 293 cells per well was plated with the Deerac Fluidics Equator. Cells were allowed to attach for 10 hours at 37C / 10% CO2. EnduRen Live Cell Substrate was added by the Equator to one plate of cells at a final concentration of 60 M and allowed to incubate with the cells for 2 hours at 37C / 10% CO2. 10
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