Navigation Links
Promegas Multiplexed Luciferase Reporter and Cell Viability Assays performed on the PHERAstar

ion note, we demonstrate the combination of several Promega cell-based assays multiplexed in both low-volume 384- and 1536-well plate formats. The BMG LABTECH PHERAstar microplate reader is used to record luminescence. Table 1 highlights the assays used in this application.


Materials and Methods

  • HEK293 cells stably transfected with a CRE/CL1 hPEST Renilla construct
  • Isoproterenol
  • Corning Low-volume 384-well plates, catalog no. 3673
  • Corning 1536-well plates, catalog no. 3937
  • Promega CellTiter-Glo Luminescent Cell Viability Assay, catalog no. G7570*
  • Promega EnduRen Live Cell Substrate, catalog no. E6482*
  • Promega ViviRen Live Cell Substrate, catalog no. E6492*
  • Deerac Fluidics Equator HTS low-volume liquid dispenser
  • BMG LABTECH PHERAstar (figure 1)
  • BMG LABTECH PHERAstar luminescence optic module

  • Multiplexing Luciferase Reporter and Cell Viability Assays
    Promegas luminescent CellTiter-Glo assay was multiplexed with either the luminescent EnduRen Live Cell Substrate, or the luminescent ViviRen Live Cell Substrate. The experimental set-up was similar for each assay combination.

    For the low-volume 384 assay format, a density of 12,000 stably transfected HEK 293 cells per well was plated with the Deerac Fluidics Equator. Cells were allowed to attach for 10 hours at 37C / 10% CO2. EnduRen Live Cell Substrate was added by the Equator to one plate of cells at a final concentration of 60 M and allowed to incubate with the cells for 2 hours at 37C / 10% CO2. 10
    '"/>

    Source:


    Page: All 1 2 3 4 5

    Related biology technology :

    1. Promegas Multiplexed Cell Viability and Apoptosis Assays performed on the PHERAstar
    2. Multiplexed Quantitative Peptide Assays for Protein Biomarkers of Cardiovascular Disease in Human Plasma
    3. Stable HeLa Luciferase Reporter Cell Lines Expressing GAL4 Fusion Transactivators
    4. Dual-Luciferase Quantitation in the LMax Microplate Luminometer (MaxLine Application Note #39)
    5. Luciferase Reporter Gene Cells and Recombinant Luciferase Dilutions Studied with the CLIPR Luciferase Assay Kit and the LMax Microplate Luminometer (MaxLine Application Note #38)
    6. New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter
    7. Versatile Reporter Vectors for Monitoring Viral Transduction
    8. New Reporter Plasmids for Studying Interferon-Stimulated Signal Transduction Pathways
    9. Genetic Reporter Systems
    10. Genetic Reporter Systems
    11. Detection of Reporter Gene Activity in Cell Cultures and Murine Epidermis After Helios Gene Gun-Mediated Particle Bombardment
    Post Your Comments:
    (Date:12/17/2014)... (PRWEB) December 17, 2014 Audacity, a ... agency and member of the Huntsworth Health Group, is ... its executive team. In her role, Gonzales will serve ... “We are incredibly honored to welcome a talent of ... and CEO, Gaëtan Fraikin. “She is a proven healthcare ...
    (Date:12/17/2014)...   Synageva BioPharma Corp. (NASDAQ: ... rare disorders, announced today its presentation at the upcoming ... San Francisco, CA. Sanj ... present on Monday, January 12, 2015, at 9:00 a.m. ... live and may be accessed from the "Webcasts & ...
    (Date:12/15/2014)... Agritech Limited (NASDAQ GS: SEED) (" Origin ", or the ... China , today announced that the Company ... 30, 2014, before the market opens on Thursday, January 8, ... January 8, 2015, at 8:00 a.m. ET / 9:00 p.m. ... To participate in the call, please dial +1-888-346-8982 in ...
    (Date:12/13/2014)... The DNA Microarray market market was ... expected to grow at a CAGR of 15.2% ... TOC of the DNA Microarray for an in-depth ... help of various tables and figures. , ... consists of instrument, consumables, and services, where the ...
    Breaking Biology Technology:Audacity Welcomes Healthcare Communications Expert Jamie Gonzales as Senior Vice President of Client Services 2Origin Agritech Limited to Announce Fiscal 2014 Annual Financial Results on January 8th, 2015 2Origin Agritech Limited to Announce Fiscal 2014 Annual Financial Results on January 8th, 2015 3DNA Microarray Market Is Expected to Grow at a CAGR of 15.2% from 2014 to 2019 - A Report by MicroMarket Monitor 2DNA Microarray Market Is Expected to Grow at a CAGR of 15.2% from 2014 to 2019 - A Report by MicroMarket Monitor 3DNA Microarray Market Is Expected to Grow at a CAGR of 15.2% from 2014 to 2019 - A Report by MicroMarket Monitor 4
    ... fusions in lysates of the yeast Saccharomyces cerevisiae using the , ... , Paul Gavin # and Mark Prescott # ... # Department of Biochemistry and Molecular ... Australia , * Technical assistance: Varian Australia Pty ...
    ... Overview , Affinity ... number of biological molecules. For example, antibodies may be purified based ... purified on the basis of its affinity for a monospecific antibody ... column with a relatively large volume. This note discusses the use ...
    ... Purpose , ... be identified and quantified more effectively and efficiently using LC/MS/MS. These ... challenges. , Overview ... safety organizations worldwide are placing great importance on the identification and ...
    Cached Biology Technology: Monitoring fluorescence resonance energy transfer (FRET) between GFP, fusions in lysates of the yeast Saccharomyces cerevisiae using the , Varian, Cary Eclipse 2 Monitoring fluorescence resonance energy transfer (FRET) between GFP, fusions in lysates of the yeast Saccharomyces cerevisiae using the , Varian, Cary Eclipse 3 Monitoring fluorescence resonance energy transfer (FRET) between GFP, fusions in lysates of the yeast Saccharomyces cerevisiae using the , Varian, Cary Eclipse 4Use of ZipTip Pipette Tips for Microscale Affinity Mass Spectrometry 2Use of ZipTip Pipette Tips for Microscale Affinity Mass Spectrometry 3Identification of Insecticides and Herbicides by LC/MS/MS Using the API 2000 LC/MS/MS System 2
    (Date:11/21/2014)... , Nov. 20, 2014   Atmel® Corporation ... microcontroller (MCU) and touch technology solutions, today launched the ... with the widest V cc range from ... accuracy and faster I 2 C bus communication speeds, ... EEPROM memory making them ideal for consumer, industrial, computer, ...
    (Date:11/18/2014)... , Nov. 17, 2014 The Parenteral Drug Association ... regulatory agencies will speak and at least seven more will ... at the Omni Shoreham Hotel in Washington D.C. ... again to have significant support from the regulatory agencies in ... Europe in our effort to help advance the ...
    (Date:11/12/2014)... , Nov. 12, 2014 Crossmatch™, a ... that its U.are.U ® fingerprint readers have been ... in Central Mexico . The bakery ... to eliminate payroll issues caused by employees clocking in ... the U.are.U fingerprint readers, Montparnasse relied on paper timecards ...
    Breaking Biology News(10 mins):Atmel Launches Industry's First Wide-V(cc) Low-Power Temperature Sensor Family 2Atmel Launches Industry's First Wide-V(cc) Low-Power Temperature Sensor Family 3Atmel Launches Industry's First Wide-V(cc) Low-Power Temperature Sensor Family 4FDA's Janet Woodcock, EMA's Emer Cooke Headline PDA Quality Metrics Conference 2Montparnasse Pasteleria Achieves Time and Attendance Transparency with Crossmatch U.are.U Fingerprint Readers 2
    ... of Rochester Medical Center scientists have demonstrated a new ... sometimes dangerous E. coli bacteria. , The unique technology ... of the sensing system that also includes a silicon ... and Bioelectronics published an article on the technology in ...
    ... clinical trial demonstrate that high doses of an experimental ... healthy adults. , Approximately half of those volunteers ... the highest dosage of the vaccine tested in the ... predict would neutralize the virus. The National Institute of ...
    ... estate may be its most important feature to many ... San Diego (UCSD) and the University of Virginia have ... distributed along the chromosomes of bacteria and simpler organisms ... , In a paper published Jan. 13 online in ...
    Cached Biology News:Rochester scientists develop fast-working biosensor 2H5N1 avian flu virus vaccine induces immune responses in healthy adults 2H5N1 avian flu virus vaccine induces immune responses in healthy adults 3Patterns in genome organization may partially explain how microbial cells work 2Patterns in genome organization may partially explain how microbial cells work 3
    ... Using the New FAST(tm) Frame Multi-Slide Plate! ... designed to hold four, 16-pad FAST Slides ... a microplate footprint for high throughput processing ... standards recommended by the Society of Biomolecular ...
    ... Package Superior Protein Detection and Quantitation ... 2D Proteomic Imaging System is a highly ... general life science research applications. ProXPRESS 2D ... all dyes in the UV and VIS ...
    fully automated system for the processing of microarrays on microscope slides...
    ... Blot includes 7 lanes ... from Alzheimer's-diseased brain tissue ... gyrus, frontal lobe, temporal ... lobe and cerebellum. Patent/trademark/license ...
    Biology Products: