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Promegas Multiplexed Cell Viability and Apoptosis Assays performed on the PHERAstar

ell. Using a non-lytic assay first, such as the CellTiter-Blue assay used here, allows for the sequential multiplexing of several different reagents within the same well. Multiplexed cell-based assays allow for multiple parameters to be measured within the same well. One example of this is to determine the method of cell death following a certain treatment protocol. By performing two assays within the same well, information on mode of action of drugs could be obtained faster and with less consumable usage. Data from miniaturized assays in 1536-well format are comparable to those run in low volume 384, indicating that smaller assay volume does not compromise the results obtained in higher density formats. The data generated here also showcases the ability of BMG LABTECHs multifunctional PHERAstar to record multiple output signals from the same assay well.


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