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Promegas Multiplexed Cell Viability and Apoptosis Assays performed on the PHERAstar

his application note, we demonstrate the combination of several Promega cell-based assays multiplexed in both low-volume 384 and 1536-well plate formats. The BMG LABTECH PHERAstar microplate reader is used to record both luminescence and fluorescence, depending on the multiplex combination. Table 1 highlights the assays used in this application.


Materials and Methods

  • Jurkat cells
  • Anti-FAS monoclonal antibody
  • Corning Low-volume 384-well plates, catalog no. 3673
  • Corning 1536-well plates, catalog no. 3937
  • Promega Caspase-Glo 3/7 Assay System, catalog no. G8091*
  • Promega CellTiter-Blue Cell Viability Assay, catalog no. G8080
  • Promega Apo-ONE Homogeneous Caspase 3/7 Assay, catalog no. G7790
  • Deerac Fluidics Equator HTS low-volume liquid dispenser
  • BMG LABTECH PHERAstar (figure 1)
  • BMG LABTECH PHERAstar luminescence optic module
  • BMG LABTECH PHERAstar fluorescence optic module (Ex: 485 nm; Em: 520 nm)
  • BMG LABTECH PHERAstar fluorescence optic modules (Ex: 540 nm; Em: 590 nm)

  • Multiplexing Cell Viability and Apoptosis Assays
    Promegas fluorescent CellTiter-Blue cell viability assay was multiplexed with either the luminescent Caspase-Glo 3/7 assay, or the fluorescent Apo-ONE assay. The experimental set-up was similar for each assay combination.

    For the low-volume 384 assay format, a density of 10,000 Jurkat cells per well was plated with the Deerac Fluidics Equator. Next, a range of anti-FAS monoclonal antib
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