( of AMV reverse transcriptase for the cD...)Product overview

of AMV reverse transcriptase for the cDNA synthesis step to reduce secondary structure. AMV also provides better processivity and fuller length transcripts when compared to RNase H negative M-MuLV reverse transcriptase mutants. For the final PCR step, the Titan system uses the enhanced fidelity and high yield of the Expand High Fidelity enzyme blend.

Uracil-DNA Glycosylase

Uracil-DNA glycosylase is used for prevention of carry-over contamination in PCR. The enzyme modifies all uracil-containing products from previous PCRs which then can be hydrolyzed by heat under alkaline conditions.

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