( ...)Product overview

Below please find an alphabetical list of key Roche Applied Science products for nucleic acid amplification. For detailed information about a product please follow the respective link. Protector RNase Inhibitor

• is a new type of RNase inhibitor and inhibits the widest known spectrum of RNases (RNaseA, RNaseB, RNaseT1, RNaseT2, RNase1).
  
• does not interfere with the performance of enzymes in an assay - even when high amounts (e.g. up to 16-fold the standard concentration) of Protector RNase Inhibitor are needed to protect difficult RNA samples.
  
• is fully active over a wide pH range of 5,0 - 9,0.sdfsdfsd.
  
• is fully active over a wide temperature range of 25C - 55C. Even at 60C, some RNase Inhibition is still measured.This is advantageous when performing reverse transcription reactions at elevated temperatures to overcome secondary structure in RNA (e.g. in combination with AMV Reverse Transcriptase to generate full length transcripts).

making it the product of choice for the secure protection of all your RNA samples.

C. therm. Polymerase One-Step RT-PCR System

C. therm. Polymerase easily overcomes structure and sensitivity problems encountered during RT-PCR reactions. This magnesium-dependent polymerase exhibits optimal activity between 60C and 70C (maximum of 72C), dramatically reducing RNA secondary structure and increasing transcription efficiency.

C. therm Polymerase for Reverse Transcription in Two-Step RT-PCR

C. therm. Polymerase easily overcomes structure and sensitivity problems encountered during RT-PCR reactions. This magnesium-dependent polymerase exhibits optimal activity between 60C and 70C (maximum of 72C), dramatically reducing RNA secondary structure and increasing transcription efficiency.

Expand High Fidelity PCR System

Expand High Fidelity PCR System delivers superior PCR results with a greater than two-fold higher yield and three-fold greater fidelity than Taq DNA polymerase. More sensitive than Taq DNA polymerase, Expand High Fidelity improves PCR results for genomic targets up to 5 kb in length.

Expand High Fidelity ^PLUS PCR System

Roche Applied Science, which pioneered the blending of thermostable DNA Polymerases with the Expand High Fidelity and Expand Long Template PCR Systems, now introduces the Expand High Fidelity PLUS PCR System - the first product of a family of next generation PCR Blends. This new blend combines Taq DNA Polymerase with a novel proofreading protein, isolated and characterized by Roche Applied Science. This protein mediates proofreading activity but has no polymerase activity itself. The synergy between the mechanism of the proofreading protein and the processivity of Taq DNA Polymerase is the key to Expand HiFiPLUS Enzyme Blends unique ability to

• Deliver higher yields
• Improve accuracy for high-fidelity applications, and
• Incorporate dUTP to prevent carry-over Contamination

for amplification reactions up to 5 kb.

Expand Long Template PCR System

Expand Long Template PCR System simplifies long and accurate PCR reactions. This optimized enzyme blend and three-buffer set enables PCR products from five to greater than twenty kilobases in length to be produced. Even if Taq were able to amplify such long fragments, Expand Long Template would deliver twice the fidelity and much higher yields.

Expand 20 kb^PLUS PCR System

Expand 20 kb^PLUS PCR System is a unique enzyme blend containing Taq and Pwo DNA Polymerase and another thermostable enzyme. The system produces extra long PCR products from genomic DNA (>20 kb fragments from human genomic DNA or up to 47 kb fragments from lambda-DNA). The enzyme blend may be used for any application that requires amplification of very long DNA sequences.

FastStart Taq DNA Polymerase

Obtain superior results in routine, multiplex, nested, high-throughput, or complex genomic PCR up to 3 kb with FastStart Polymerase. This modified recombinant Taq DNA Polymerase is inactive at temperatures below 75C but is activated by a 4-minute heat-activation step at 95C. Minimize non-specific amplification products and amplify even the most problematic templates (GC-rich sequences, repeats, high secondary structure) with the optimized buffer system and the revolutionary GC-RICH Resolution Solution.

GC-RICH PCR System

The GC-Rich PCR System, a blend of Taq DNA polymerase and a proofreading polymerase, powers through templates that are difficult or impossible to amplify with other polymerases or additives. The enhanced processivity of the blend and the unique GC-RICH Resolution Solution combine to deliver superior performance especially from problematic templates.

High Fidelity PCR Master

High Fidelity PCR Master contains all necessary reagents (buffers, nucleotides and enzymes) needed for PCR. The mix contains a unique enzyme blend of Taq DNA Polymerase and Pwo DNA Polymerase[a thermostable enzyme with a proofreading (3'5' exonuclease) activity]. High Fidelity PCR Master amplifies with threefold greater accuracy than Taq DNA Polymerase alone and gives better results with impure templates because it is less affected by contaminants in a
DNA preparation than other DNA polymerases.

PCR Core Kit^PLUS

The PCR Core KitPLUS is a convenient kit with ready-to-use reagents which has been specially designed for performing PCR with Taq polymerase and carry-over prevention. The kit contains heat-labile UNG which allows reliable removal of carry-over contaminants.

PCR-Grade Deoxynucleotides

Produce sensitive and consistent results with the newly developed PCR grade deoxy-nucleotides from Roche Applied Science. Improve performance and sensitivity in PCR and RT-PCR assays, even when analyzing low copy number targets. New production procedures eliminate dNTPs with modified bases, and tetra- and pyrophosphate contamination which often inhibit PCR and labeling reactions. Roche Molecular Biochemcals is the only supplier to function test each lot of dNTPs for RNase, DNase, and nicking activity.

PCR-Grade Water

Like all components of a PCR or RT-PCR assay, the water component must be of a high purity. Standard laboratory water is often contaminated with DNA, RNA or nucleases. These contaminants often contribute to suboptimal amplification reactions. This PCR-grade water is specially purified, deionized, double-distilled, and autoclaved. The unique production process also ensures that no detectable amounts of nucleic acids are included, as tested with conventional thermal cyclers and in highly sensitive assays with the LightCycler Instrument and related LightCycler kits. In addition, this water is guaranteed to be free of detectable endonucleases, ribonucleases and nicking activity.

PCR Master

The PCR Master Kit contains 10 vials of ready-to-use PCR reaction mix including Taq DNA polymerase sufficient for 100 PCR reactions. It amplifies genomic targets up to 3 kb with good yield and specificity and is capable of amplifying lambda DNA up to 10 kb.

Taq DNA Polymerase

Get the most consistent results out of your PCR reactions by using Roche Applied Science (RASs) Taq DNA Polymerase, which is held to our rigorous purity and quality standards. Before it arrives in your laboratory, every lot of Roche Taq DNA polymerase must pass tests for functionality and contaminating activities. Thus, you can be confident that you will obtain reliable, consistent results with every lot of RAS Taq DNA Polymerase.

Pwo DNA Polymerase

initially isolated from the hyperthermophilic archaebacterium Pyrococcus woesei - combines an efficient 5- 3thermostable polymerase activity with a highly processive 3- 5exonuclease, or "proofreading" activity. Roche Applied Science (RASs) recombinant preparation minimizes the incorporation of incorrect bases and delivers a 18-fold higher accuracy than Taq DNA Polymerase and is therefore the product of choice when DNA needs to be amplified with the highest possible accuracy.

Titan One Tube RT-PCR System/Kit

Using three distinct enzymes, the Titan RT-PCR System offers unmatched sensitivity and reproducibility in one tube RT-PCR. The Titan system exploits the superior thermal stability of AMV reverse transcriptase for the cDNA synthesis step to reduce secondary structure. AMV also provides better processivity and fuller length transcripts when compared to RNase H negative M-MuLV reverse transcriptase mutants. For the final PCR step, the Titan system uses the enhanced fidelity and high yield of the Expand High Fidelity enzyme blend.

Uracil-DNA Glycosylase

Uracil-DNA glycosylase is used for prevention of carry-over contamination in PCR. The enzyme modifies all uracil-containing products from previous PCRs which then can be hydrolyzed by heat under alkaline conditions.

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