Navigation Links
Principles of Curve Fitting for Multiplex Sandwich Immunoassays, Rev B

on may not be as useful when analyzing samples in a multiplex setting (e.g., Bio-Plex cytokine assays are available in an 18-plex panel). Each analyte exhibits a different response and resulting linear range, and as a result it is difficult to select a universal set of standards that covers all of the analytes in a panel. This problem may be circumvented in the data analysis step of Bio-Plex Manager by deleting specific standards for each analyte; however, selection of standards that yield an R2 value >0.99 for each analyte is a tedious and time-consuming task. It is also important to analyze the backcalculated standards when using linear regression. Even if the R2 value is very high (>0.99), the accuracy of the fit as determined by standards recovery may indicate otherwise. For example, Figure 6 shows a cytokine assay exhibiting an R2 value of 1.000, indicating an excellent fit through the points; however, the associated backcalculated standards data (Table 1) indicate that the goodness of fit is not optimal throughout the entire range of the standards.

Logistic regression yields accurate quantitation across a wider range of concentrations compared to linear regression. This is the primary advantage of using a logistic regression. In Table 2, the standards recovery of a mouse cytokine assay using both linear and logistic regression methods is shown. The cells corresponding to 70130% standards recovery for linear and logistic regression are shaded. The range using logistic regression is much broader compared to the linear regression range. These data are shown graphically in Figure 7. The red bars indicate the range of concentrations showing 70130% recovery using linear re
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. Principles and applications of electrical cell fusion
2. Principles and applications of electrical cell fusion
3. Identification of Nonspecific Products Using Melt-Curve Analysis on the iCycler iQ Detection System, Rev A
4. Real-Time PCR/Melt-Curve Analysis: SNP Detection With FRET, Rev A
5. Mx4000 Multiplex Quantitative PCR System*,PPP
6. QIAGEN Multiplex PCR Handbook
7. QIAGEN Multiplex PCR Kit
8. Real-Time Multiplex PCR from Genomic DNA Using the iCycler iQ Detection System
9. Correlation of Bio-Plex Suspension Array Reader Validation With Multiplex Cytokine Assay Performance, Rev A
10. Development of a Multiplex Bead-Based Assay for Antibody Screening of a Nonhuman Primate Colony on the Bio-Plex System
11. Feasibility of Multiplexing Bio-Plex Total Target and Phosphoprotein Assays
Post Your Comments:
(Date:10/22/2014)... (PRWEB) October 22, 2014 Currently ... Sartorius, A & D Weighing, Rice Lake, and ... Semi-Micro Balance . The Sartorius CPA Semi-Micro Balance ... affordable high-quality, precise, and user-friendly laboratory balance. , ... laboratory equipment, their laboratory balances are well known ...
(Date:10/22/2014)... (PRWEB) October 22, 2014 According ... Market by Service Type (Consulting, Integration, Deployment & ... by Deployment Model (Public, Private, Others (Hybrid & ... MarketsandMarkets, defines and segments the Cloud Professional Services ... and forecasting of revenues. It also identifies the ...
(Date:10/22/2014)... 22, 2014 Nuvilex, Inc. (OTCQB: NVLX) – ... people worldwide are living with diabetes, with  that number ... The global market for diabetes treatments is approximately $500 ... worldwide died from pancreatic cancer.  Pancreatic cancer is the ... in the United States , and ...
(Date:10/20/2014)... (PRWEB) October 20, 2014 The ... (Medication Dispensing Systems, Packaging and Labeling Systems, Table-top ... Automation) - Global Forecasts & Trends to 2019” ... and opportunities in North America, Europe, Asia-Pacific, and ... 101 market tables and 30 figures spread through ...
Breaking Biology Technology:Pipette.com Launches the Addition of Sartorius CPA Semi-Micro Balance to Their Product Portfolio with Special Promotional Pricing 2Pipette.com Launches the Addition of Sartorius CPA Semi-Micro Balance to Their Product Portfolio with Special Promotional Pricing 3Cloud Professional Services Market by Public, Private, Hybrid & Community Expected to Reach $34.41 Billion by 2019 - New Report by MarketsandMarkets 2Cloud Professional Services Market by Public, Private, Hybrid & Community Expected to Reach $34.41 Billion by 2019 - New Report by MarketsandMarkets 3Cloud Professional Services Market by Public, Private, Hybrid & Community Expected to Reach $34.41 Billion by 2019 - New Report by MarketsandMarkets 4Cloud Professional Services Market by Public, Private, Hybrid & Community Expected to Reach $34.41 Billion by 2019 - New Report by MarketsandMarkets 5Nuvilex Brief Analyst Report: Thinking Outside the Box by BrokerBank Securities, Inc. 2Pharmacy Automation Systems Market worth $4,566.2 Million by 2019 - New Research Report by MarketsandMarkets 2Pharmacy Automation Systems Market worth $4,566.2 Million by 2019 - New Research Report by MarketsandMarkets 3Pharmacy Automation Systems Market worth $4,566.2 Million by 2019 - New Research Report by MarketsandMarkets 4
... vein treatment methods led by Dr. Sinjae Hyun, associate professor of biomedical ... he will present this summer to his research collaborators and the company. ... ... California company aided in the research of vein treatment methods ...
... Therapeutics, Inc. (CTI),(Nasdaq and MTA: CTIC) announced today ... accepted for filing and review, and has granted,priority ... for use of Zevalin(R) ([90Y]-ibritumomab tiuxetan) as consolidation,therapy ... achieve,a response to first-line therapy. Priority review is ...
... ENCO ), a full service multinational clinical ... many of the world,s leading,pharmaceutical and biotechnology companies, today ... from Nasdaq stating that since it had not yet,filed ... 2008 as of the,date of the letter, it was ...
Cached Biology Technology:Gift Aids Professor's Research into Vein Treatment 2Gift Aids Professor's Research into Vein Treatment 3Gift Aids Professor's Research into Vein Treatment 4FDA Accepts Cell Therapeutics' Zevalin sBLA and Grants Priority Review 2FDA Accepts Cell Therapeutics' Zevalin sBLA and Grants Priority Review 3FDA Accepts Cell Therapeutics' Zevalin sBLA and Grants Priority Review 4FDA Accepts Cell Therapeutics' Zevalin sBLA and Grants Priority Review 5Encorium Announces Receipt of Letter from Nasdaq Regarding its Third Quarter 2008 Form 10-Q Filing 2Encorium Announces Receipt of Letter from Nasdaq Regarding its Third Quarter 2008 Form 10-Q Filing 3
(Date:10/22/2014)... Medicine® scientists helps explain how bipolar disorder affects the ... therapies to treat the mental illness. , Scientists ... method recognized with the 2014 Nobel Prize in ... from mice with bipolar-like behaviors. In the synapses (where ... structures with concentrated levels of ANK3 -- the gene ...
(Date:10/22/2014)... , Oct. 20, 2014  Leading identity analyst firm ... half of the world,s population will have a chip-based ... Europe . Asia , ... market accounting for more than 60% of all National ... Acuity,s report  -- "The Global National eID ...
(Date:10/19/2014)... in India over the last 30 years contributed ... growth during that time, according to a new ... Change ., "Energy access is fundamental to development: ... including education, communication, and health," says IIASA researcher ... increased energy access is widely agreed to be ...
Breaking Biology News(10 mins):Bipolar disorder discovery at the nano level 2Half the World's Population Will Have Chip-Based National Electronic Identity (eID) Cards by 2018 2Improved electricity access has little impact on climate change 2
... DURHAM, N.C. Under the microscope, the bacteria start dividing ... on. But then individual cells begin "popping," like circus balloons ... Duke University bioengineers who captured it on video, turns out ... must be considered by scientists creating living, synthetic circuits out ...
... professor Kam Tang of the Virginia Institute of Marine ... fleas" play an important role in carrying hitchhiking bacteria ... article, "Bacteria dispersal by hitchhiking on zooplankton," appeared in ... the National Academy of Sciences . It was co-authored ...
... - Greenhouse gas markets, where invisible gases are ... people. Farmers can make money on these markets, ... methane capture technologies in animal-based systems, no-till farming, ... extension educators, offset aggregators, and other stakeholders need ...
Cached Biology News:Popping cells surprise living circuits creators 2Popping cells surprise living circuits creators 3Study shows that hitchhiking bacteria can go against the flow 2Study shows that hitchhiking bacteria can go against the flow 3Greenhouse gas calculator connects farming practices with carbon credits 2