Navigation Links
Principles of Curve Fitting for Multiplex Sandwich Immunoassays, Rev B

on may not be as useful when analyzing samples in a multiplex setting (e.g., Bio-Plex cytokine assays are available in an 18-plex panel). Each analyte exhibits a different response and resulting linear range, and as a result it is difficult to select a universal set of standards that covers all of the analytes in a panel. This problem may be circumvented in the data analysis step of Bio-Plex Manager by deleting specific standards for each analyte; however, selection of standards that yield an R2 value >0.99 for each analyte is a tedious and time-consuming task. It is also important to analyze the backcalculated standards when using linear regression. Even if the R2 value is very high (>0.99), the accuracy of the fit as determined by standards recovery may indicate otherwise. For example, Figure 6 shows a cytokine assay exhibiting an R2 value of 1.000, indicating an excellent fit through the points; however, the associated backcalculated standards data (Table 1) indicate that the goodness of fit is not optimal throughout the entire range of the standards.

Logistic regression yields accurate quantitation across a wider range of concentrations compared to linear regression. This is the primary advantage of using a logistic regression. In Table 2, the standards recovery of a mouse cytokine assay using both linear and logistic regression methods is shown. The cells corresponding to 70130% standards recovery for linear and logistic regression are shaded. The range using logistic regression is much broader compared to the linear regression range. These data are shown graphically in Figure 7. The red bars indicate the range of concentrations showing 70130% recovery using linear re
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. Principles and applications of electrical cell fusion
2. Principles and applications of electrical cell fusion
3. Identification of Nonspecific Products Using Melt-Curve Analysis on the iCycler iQ Detection System, Rev A
4. Real-Time PCR/Melt-Curve Analysis: SNP Detection With FRET, Rev A
5. Mx4000 Multiplex Quantitative PCR System*,PPP
6. QIAGEN Multiplex PCR Handbook
7. QIAGEN Multiplex PCR Kit
8. Real-Time Multiplex PCR from Genomic DNA Using the iCycler iQ Detection System
9. Correlation of Bio-Plex Suspension Array Reader Validation With Multiplex Cytokine Assay Performance, Rev A
10. Development of a Multiplex Bead-Based Assay for Antibody Screening of a Nonhuman Primate Colony on the Bio-Plex System
11. Feasibility of Multiplexing Bio-Plex Total Target and Phosphoprotein Assays
Post Your Comments:
(Date:1/15/2014)... Rochester, NY (PRWEB) January 15, 2014 The ... cause a major disease. One of these latent viruses is ... is rheumatoid arthritis (RA). Rheumatoid arthritis (RA) is a chronic ... the theory, a study found that RA patients have high ...
(Date:1/15/2014)... (PRWEB) January 15, 2014 2013 was ... Scottsdale’s Brain State Technologies®. They saw continued independent research ... Center who were awarded a $1 million grant from ... in “Brain and Behavior” a peer reviewed journal, Amy ...
(Date:1/15/2014)... AudioNotch is the internet's leading provider ... treatment of tinnitus. Patients listen to sound therapy that ... period of weeks to months, their tinnitus volume decreases. , ... Notched Music and Notched White Noise. Now, AudioNotch is pleased ...
(Date:1/14/2014)... Date: Friday, April 11, 2014 , Time: ... 1360 Almshouse Road, Warrington, Pa. , Details: The ... to finding a cure for hepatitis B and improving the ... annual Crystal Ball on Friday, April 11 at Warrington Country ...
Breaking Biology Technology:Study: Rheumatoid Arthritis (RA) Patients Have EBV; The CBCD Says this is Consistent with Microcompetition 2Study: Rheumatoid Arthritis (RA) Patients Have EBV; The CBCD Says this is Consistent with Microcompetition 3Dynamic Innovative Technology Showcased at Scottsdale Company’s Open House 2Dynamic Innovative Technology Showcased at Scottsdale Company’s Open House 3Hepatitis B Foundation to Host Annual Crystal Ball Gala 2
... the Risks of DVT to Mark U.S. House of ... Coalition to Prevent DVT today announced the launch of ... deep-vein thrombosis (DVT) and to encourage dialogue between healthcare ... A customized recreational vehicle will visit hospitals and ...
... Christine Lawless, MD, has accepted the position as Medical ... medical education (CME), beginning March 2, 2009. Recognized as ... cardiology, Dr. Lawless recently completed her clinical tenure at ... she was Associate Professor of Internal Medicine and Co-Director ...
... March 10 WaferGen Biosystems, Inc. (OTC Bulletin ... analysis systems, today announced that Kumar Kastury, Ph.D., ... will present data on the company,s SmartChip(TM) Real-Time ... Anniversary Quantitative PCR Conference. The conference will ...
Cached Biology Technology:The Coalition to Prevent Deep-Vein Thrombosis (DVT) RV Tour Takes to the Road to Educate the Nation About the Importance of DVT Risk Assessment 2The Coalition to Prevent Deep-Vein Thrombosis (DVT) RV Tour Takes to the Road to Educate the Nation About the Importance of DVT Risk Assessment 3The Coalition to Prevent Deep-Vein Thrombosis (DVT) RV Tour Takes to the Road to Educate the Nation About the Importance of DVT Risk Assessment 4The Coalition to Prevent Deep-Vein Thrombosis (DVT) RV Tour Takes to the Road to Educate the Nation About the Importance of DVT Risk Assessment 5Former President of Sports Cardiology Consultants, LLC Christine Lawless, MD, MBA, ABCL, FACC, FACSM, Named Medical Director of CME Enterprise 2WaferGen to Present Data on SmartChip(TM) Real-Time PCR System at Cambridge Healthtech Institute's Quantitative PCR Conference 2WaferGen to Present Data on SmartChip(TM) Real-Time PCR System at Cambridge Healthtech Institute's Quantitative PCR Conference 3WaferGen to Present Data on SmartChip(TM) Real-Time PCR System at Cambridge Healthtech Institute's Quantitative PCR Conference 4
(Date:4/17/2014)... One day about eight years ago, Katia Silvera , ... her father were on a field trip in a mountainous ... they had never seen before. , Unable to identify it, ... The orchid turned out to be an unnamed species. So ... . , "Lophiaris" is the genus name, comprising about 40 ...
(Date:4/17/2014)... If a restaurant ... he can expect his premises to be trashed. Warnings like ... is enough to make restaurant owners pay up. Similarly, mafia-like ... in other birds, nests. If the host birds throw the ... destroying the entire nest. Consequently, it is beneficial for hosts ...
(Date:4/17/2014)... fragmentation of forests in the Amazon help create ... to rapid and widespread forest loss during drought ... findings show that forests in the Amazon could ... with forest fires lead to large-scale loss of ... assistant professor of geography, Penn State. , "We ...
Breaking Biology News(10 mins):Orchid named after UC Riverside researcher 2Orchid named after UC Riverside researcher 3Fear of the cuckoo mafia 2Fear of the cuckoo mafia 3Drought and fire in the Amazon lead to sharp increases in forest tree mortality 2Drought and fire in the Amazon lead to sharp increases in forest tree mortality 3
... An international team of investigators at Sanford-Burnham Medical Research ... Centre for Molecular Life Sciences and other organizations have ... also plays a significant role in Frank-Ter Haar syndrome ... on February 12 in the American Journal of ...
... CINCINNATI -- Scientists have discovered a molecular pathway that works ... may lead to new therapies for repairing injury in a ... week,s Proceedings of the National Academy of Sciences ... Children,s Hospital Medical Center and the Brigham & Women,s Hospital ...
... reveals that a common underlying mechanism is shared by ... complex defects in brain development and function. Rett syndrome ... Retardation, X-linked syndrome (ATR-X) have each been linked with ... DNA that make up chromosomes and control how genetic ...
Cached Biology News:Researchers identify mechanism for Frank-Ter Haar syndrome 2Scientists discover molecular pathway for organ tissue regeneration and repair 2A common thread links multiple human cognitive disorders 2
iNOS/NOS Type II Purified Anti-Mouse, Anti-Rat, Anti-Human clone Polyclonal, Isotype Rabbit Ig, 150 µg Consult technical datasheet for details....
...
... gradient system uses two Adept CE 4100 ... CE 4200 dual wavelength detector for added flexibility. ... by PowerStream data processing software and interface unit, ... The supplied analytical pumps may be substituted ...
... Highly nutritious peptone, used ... production of bacterial toxins, ... of bacteria with high ... Haemophilus, Neisseria, Pasteurella, Salmonella ...
Biology Products: