Navigation Links
Principles of Curve Fitting for Multiplex Sandwich Immunoassays, Rev B

Diana Davis, PhD, Aiguo Zhang, PhD, Chloe Etienne, Ivan Huang, and Michele Malit, Bio-Rad Laboratories, Inc., 2000 Alfred Nobel Drive, Hercules, CA 94547 USA


Introduction
Bio-Plex cytokine assays are bead-based multiplex sandwich immunoassays. The concentrations of analytes in a Bio-Plex cytokine immunoassay are quantitated using a calibration or standard curve. More specifically, a series of known concentrations of an analyte is used to construct a plot of signal intensity vs. concentration. The plot is mathematically modeled to derive an equation that may be used to predict the concentrations of unknown samples. The type of mathematical or curve-fitting model as well as the fit of the model have a direct effect on the accuracy of the results. Therefore, curve fitting is a critical component of immunoassay performance. In this technical note, curvefitting methods as well as methods used to determine the quality of the curve fitting are discussed. Advantages and disadvantages of each method and the effects on the predicted cytokine concentrations are illustrated using representative Bio-Plex cytokine assay data.


Methods of Curve Fitting
Linear Regression
The simplest method for determining concentrations from a standard curve is to construct a plot of the concentration vs. the response using the linear portion of the response curve. This method has been used traditionally to quantitate results of ELISA and other immunoassays (Nix and Wild 2001). An example of a GM-CSF Bio-Plex cytokine assay plotted using a linear regression is shown in Figure 1. The R2 value is used to determine the overall goodness of the linea
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. Principles and applications of electrical cell fusion
2. Principles and applications of electrical cell fusion
3. Identification of Nonspecific Products Using Melt-Curve Analysis on the iCycler iQ Detection System, Rev A
4. Real-Time PCR/Melt-Curve Analysis: SNP Detection With FRET, Rev A
5. Mx4000 Multiplex Quantitative PCR System*,PPP
6. QIAGEN Multiplex PCR Handbook
7. QIAGEN Multiplex PCR Kit
8. Real-Time Multiplex PCR from Genomic DNA Using the iCycler iQ Detection System
9. Correlation of Bio-Plex Suspension Array Reader Validation With Multiplex Cytokine Assay Performance, Rev A
10. Development of a Multiplex Bead-Based Assay for Antibody Screening of a Nonhuman Primate Colony on the Bio-Plex System
11. Feasibility of Multiplexing Bio-Plex Total Target and Phosphoprotein Assays
Post Your Comments:
(Date:12/17/2014)... Based on the revenues gained from ... is dominated by four major players contributing to ... players include GE Healthcare (U.K.), Siemens AG (Germany), ... Full Report Copy at http://www.marketsandmarkets.com/Market-Reports/surgical-imaging-market-210534462.html , ... attributed to its strong product portfolio, which includes ...
(Date:12/17/2014)... December 17, 2014 Once more, EMAAR ... a larger packaged waste water treatment plant to phase ... Economic City (KAEC). Two years ago Bioshaft successfully supplied ... Development, serving two residential towers with an occupancy of ... a quarter million gallons per day and was signed ...
(Date:12/17/2014)... 17, 2014 Audacity, a leading California-based ... member of the Huntsworth Health Group, is proud to ... team. In her role, Gonzales will serve as Senior ... incredibly honored to welcome a talent of Jamie’s caliber ... Gaëtan Fraikin. “She is a proven healthcare innovation leader ...
(Date:12/17/2014)... 17, 2014 Tigercat Pharma, Inc. today ... evaluating the investigational oral NK-1 receptor antagonist VPD-737 ... chronic itch who are unresponsive or inadequately responsive ... steroids and antihistamines. The primary ... Scale (VAS) itch score from baseline, comparing drug ...
Breaking Biology Technology:Global Refurbished Surgical Imaging Market by Product & Application is Estimated to Reach $1.25 Billion by 2019 - New Research Report by MarketsandMarkets 2Global Refurbished Surgical Imaging Market by Product & Application is Estimated to Reach $1.25 Billion by 2019 - New Research Report by MarketsandMarkets 3Global Refurbished Surgical Imaging Market by Product & Application is Estimated to Reach $1.25 Billion by 2019 - New Research Report by MarketsandMarkets 4Audacity Welcomes Healthcare Communications Expert Jamie Gonzales as Senior Vice President of Client Services 2Velocity Pharmaceutical Development, LLC and Tigercat Pharma, Inc. Announce Phase 2 Results for VPD-737 in Patients With Chronic Pruritus 2Velocity Pharmaceutical Development, LLC and Tigercat Pharma, Inc. Announce Phase 2 Results for VPD-737 in Patients With Chronic Pruritus 3Velocity Pharmaceutical Development, LLC and Tigercat Pharma, Inc. Announce Phase 2 Results for VPD-737 in Patients With Chronic Pruritus 4
... Chest Physicians (ACCP) Guidelines Will Increase ... Use of Trellis(R) Peripheral Infusion System, NORTHBROOK, ... (ACCP) has published new evidence-based clinical guidelines for,physicians that ... for acute iliofemoral deep vein thrombosis (DVT) in,conjunction with ...
... are everywhere from the air we breathe and the food ... are innocuous or even beneficial, and only a handful pose any ... unwanted intruder? , Research from the University of Wisconsin-Madison suggests ... , A study appearing online this week in the Proceedings ...
... Ardea Biosciences, Inc.,(Nasdaq: RDEA ) today ... the,Company,s novel investigational non-nucleoside reverse transcriptase,inhibitor (NNRTI) for ... Annual ICAAC/IDSA 46th Annual Meeting in Washington, DC ... of HIV Research, Chelsea and Westminster,Hospital, will present ...
Cached Biology Technology:Chest Physicians Now Suggest Early Intervention for Certain Blood Clots in DVT 2Symbiotic microbes induce profound genetic changes in their hosts 2Ardea to Present Data on Lead HIV Candidate, RDEA806, at ICAAC/IDSA Joint Meeting 2Ardea to Present Data on Lead HIV Candidate, RDEA806, at ICAAC/IDSA Joint Meeting 3
(Date:12/10/2014)... -- Valencell, a leader in performance biometric data sensor technology, ... its licensees for highly accurate, clinically validated biometric technology. ... from fitness and health sectors, but first responders/military and ... only as useful as the biometric data it delivers. ... long-term mass consumer adoption of wearable products," said Dr. ...
(Date:12/10/2014)... California , December 9, 2014 ... with ITN,s BCARD Platform   ... cloud-based enterprise meeting scheduling solutions for business-to-business (B2B) ... the world leader in mobile near-field communication (NFC), ...      (Logo: http://photos.prnewswire.com/prnh/20140612/691055) , ...
(Date:12/3/2014)... 2014   Marvin Test Solutions , a ... for military, aerospace, and manufacturing organizations, today announced ... TS-900 PXI semiconductor test platform . The ... systems to customers at a fraction of the ... ATE. "Our semiconductor customers asked ...
Breaking Biology News(10 mins):Wearable Technology Products Demand Highly Accurate Biometric Technology 2Jifflenow And ITN International Bring Cutting-Edge Badge Scanning Technology To B2B Events 2Jifflenow And ITN International Bring Cutting-Edge Badge Scanning Technology To B2B Events 3Marvin Test Solutions Brings New Capabilities to PXI-Based Semiconductor Test with TS-960 2Marvin Test Solutions Brings New Capabilities to PXI-Based Semiconductor Test with TS-960 3
... atomic bomb, Niels Bohr* worked tirelessly to promote a ... highly relevant in a world where internet surveillance and ... warfare raise questions about not just openness but also ... of the celebrations of the 100th anniversary of Bohr,s ...
... comprehensive, quality-controlled dataset that gives ready access to millions ... Horizon Oil Spill. The dataset, collected to support oil removal ... three year process that began with the gathering of water ... during and after the oil release in 2010. ...
... A technology-driven concept, a safe city is ... better use of intelligence. The four pillars of ... multi-agency collaboration, shared command & control, and intelligence ... a developed environment, assurance of safety for citizens ...
Cached Biology News:International Niels Bohr Conference: An open world 2NOAA launches website holding millions of chemical analyses from Deepwater Horizon oil spill 2Frost & Sullivan: Safe Cities to directly impact First Responder and Biometric markets positively 2Frost & Sullivan: Safe Cities to directly impact First Responder and Biometric markets positively 3Frost & Sullivan: Safe Cities to directly impact First Responder and Biometric markets positively 4Frost & Sullivan: Safe Cities to directly impact First Responder and Biometric markets positively 5
... Software reads and processes raw ... microarray data for analysis. ... one 1-year license for Feature ... ,one CD-ROM containing Feature Extraction ...
... CHEMICONs ELF 97 Cytological Labeling Kit provides ... the detection of cellular targets, including cell-surface ... Cytological Labeling Kit contains the patented ELF ... fluorescent yellow-green precipitate at the site of ...
... Many differences exist between the various commercial ... glass slide used, the length and GC ... and the manufacturing protocol employed all influence ... analysis. With all the different variables ...
... SYBR Safe DNA Gel Stain combo offers these benefits: ... these same products separately ... Eliminates risks to yourself, ... RNA fragments from 100 bp to >30 kb. UltraPure ...
Biology Products: