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Principles and applications of electrical cell fusion

. Small numbers of cells (20 50 l) can be fused directly in the micro fusion chamber. Helix fusion chamber

Large numbers of cells (in volumes up to 250 l) can be fused in the helix fusion chamber (Fig. 5).

The parameters obtained using the micro fusion chamber can be transferred directly.

This system thus represents a unique opportunity for simple scaling-up.

Fig. 5: The helix fusion chamber can be used to fuse large numbers of cells (in volumes up to 250 l) simultaneously. Support The Multiporator is the most compact, flexible electromanipulation device on the market. For help in the development of new applications, please contact our Hotline and our team of application specialists. Send your e-mails to: bioapps@brinkmann.com Bibliography

[1] Zimmermann, U., Pilwat, G. The relevance of electric field induced changes in the membrane structure to basic membrane research and clinical therapeutics and diagnosis. In , Kyoto, Japan, 140, 1978. Abstract IV-19-(H) of the 6th International Biophysics Congress

[2] Zimmermann, U., Vienken, J., Scheurich, P., Electric field induced fusion of biological cells. Biophys. Struct. Mech., 1980, 6 (Suppl.), 86.

[3] Zimmermann, U., Vienken, J., Pilwat, G., Development of drug carrier systems: electrical field induced effects in cell membranes. Bioelectrochem. Bioenerg.,
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