Navigation Links
Principles and applications of electrical cell fusion

Producing monoclonal antibodies

Traceable to G. Khler and C. Milstein [4], this method revolutionized the production of antibodies. Primary B lymphocytes, which produce a specific antibody, are fused with tumor cells.

The numerous fusion products are isolated and selected. These single cells are replicated as clones, which is why the term monoclonal is used.

The fusion products, described as hybridoma, stand out for two reasons: they secrete a specific antibody and they can be replicated in culture almost infinitely. This has enabled mass production of specific antibodies.

For these achievements, G. Khler, C. Milstein, and N. K. Jerne were awarded the Nobel Prize for Medicine in 1984.


Cloning mammals


The famous case of Dolly, the cloned sheep [5], would not have been possible without cell fusion. The first step in mammalian cloning is the removal of the nucleus from an oocyte. This enucleated oocyte is then fused with a somatic cell (e.g. from connective tissue). This is carried out by using electrical cell fusion because it is the only method efficient enough to produce the desired result.

The fusion product is implanted into a surrogate mother and is then carried through the full gestation period.

Cloning mammals from embryonic cells was carried out as long ago as the late 1980s [6 -10] and aroused little public interest at the time.

Vaccination against cancer
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Principles and applications of electrical cell fusion
2. Principles of Curve Fitting for Multiplex Sandwich Immunoassays, Rev B
3. An Assessment of the Eppendorf twin.tec plate performance in PCR* applications
4. Suggested applications for reverse transcriptases
5. Self-Drying Vacuum Pumps... the solution for applications producing condensation
6. A new wide field-of-view confocal imaging system and its applications in drug discovery and pathology
7. Production of Hybridomas by Electrofusion
8. Monitoring fluorescence resonance energy transfer (FRET) between GFP fusions in lysates of the yeast Saccharomyces cerevisiae using the Varian Cary Eclipse
9. Gram Quantities of MAb Produced with Simple Bioreactor in Serum-Free Perfusion Culture Replacing Ascitic Fluid Production
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Principles and applications electrical cell fusion

(Date:7/11/2014)... July 11, 2014 /PRNewswire-iReach/ -- Maxim Peptide, a website ... are used solely in scientific experiments, is currently celebrating ... in business, Maxim Peptide, which was created by the ... new and updated website that now features a special ... been updated with fascinating articles about peptide research, including ...
(Date:7/11/2014)... 11, 2014 /CNW/ - Sunovion Pharmaceuticals Canada Inc. (Sunovion) ... (eslicarbazepine acetate) for use as a once-daily adjunctive therapy ... epilepsy who are not satisfactorily controlled with conventional therapy. ... patients under 18 years of age. ... and according to Epilepsy Canada, it affects 0.6% of ...
(Date:7/10/2014)... (PRWEB) July 10, 2014 Deep ... Century Medicine Forum on ‘Commercialising Longevity Research’ and ... entrepreneurs to the London Bioscience Innovation Center for ... Foundation and Aging Analytics Ltd. The meeting highlighted ... in translational research for age-related disease, as well ...
(Date:7/10/2014)... (PRWEB) July 10, 2014 OMICS Group’s ... techniques during 18-20 August, 2014 at Double Tree by ... the recent developments in Analytical & Bioanalytical research methods ... , Speaking on this occasion, Dr. Srinubabu Gedela, MD ... Acta conference is a remarkable one in bringing a ...
Breaking Biology Technology:Maxim Peptide Celebrates Second Anniversary Online with an Updated Website and Special Deals Section 2Sunovion Pharmaceuticals Canada Inc. Announces Health Canada Approval of APTIOM(TM) (eslicarbazepine acetate) as a Once-Daily Adjunctive Treatment for Partial-Onset Seizures in Patients with Epilepsy 2Sunovion Pharmaceuticals Canada Inc. Announces Health Canada Approval of APTIOM(TM) (eslicarbazepine acetate) as a Once-Daily Adjunctive Treatment for Partial-Onset Seizures in Patients with Epilepsy 3Sunovion Pharmaceuticals Canada Inc. Announces Health Canada Approval of APTIOM(TM) (eslicarbazepine acetate) as a Once-Daily Adjunctive Treatment for Partial-Onset Seizures in Patients with Epilepsy 4DKV Sponsors Forum on Commercialising Longevity Research; Brings Together Scientists, Investors, Entrepreneurs and Celebrities 2DKV Sponsors Forum on Commercialising Longevity Research; Brings Together Scientists, Investors, Entrepreneurs and Celebrities 3DKV Sponsors Forum on Commercialising Longevity Research; Brings Together Scientists, Investors, Entrepreneurs and Celebrities 4DKV Sponsors Forum on Commercialising Longevity Research; Brings Together Scientists, Investors, Entrepreneurs and Celebrities 5DKV Sponsors Forum on Commercialising Longevity Research; Brings Together Scientists, Investors, Entrepreneurs and Celebrities 6A Critical Review of Analytical Techniques for Improved Living Standards 2A Critical Review of Analytical Techniques for Improved Living Standards 3A Critical Review of Analytical Techniques for Improved Living Standards 4
... 20, 2011 Onyx Pharmaceuticals, Inc. (Nasdaq: ONXX ... teleconference with management to discuss third quarter 2011 financial results, ... November 2, 2011, at 5:00 p.m. Eastern Time (2:00 p.m. ... 30, 2011 will be released earlier that day. ...
... Dyadic International, Inc. (OTC Pink: DYAI), a ... report its financial results for the third quarter ... conference call to discuss those financial results as ... Results Release:  After market close, Thursday, November 3, ...
... Therapeutics Corporation (NASDAQ: UTHR ) announced today ... results before market open on Thursday, October 27, 2011. ... on Thursday, October 27, 2011, at 9:00 a.m. Eastern ... international callers dialing 1-970-315-0533.  A rebroadcast of the teleconference ...
Cached Biology Technology:
(Date:7/11/2014)... for Bone and Mineral Research awarded Michael F. Holick, ... with the 2014 Louis V. Avioli Award. Holick, a ... internationally known for revolutionizing the understanding of vitamin D ... award honors a member of the American Society for ... bone and mineral basic research. It is named for ...
(Date:7/11/2014)... release is available in German . ... a method that uses the refraction of X-rays through ... images produced with this method are often of much ... in the team of Prof. Franz Pfeiffer are particularly ... and therapy including X-ray phase-contrast imaging. One main ...
(Date:7/11/2014)... at the National Institute of Standards and Technology ... work more effectively, they often prove that necessity ... the case recently for M. Lorna De Leoz ... growing specialization of glycomics. Glycomics is the study ... that are attached to proteins and lipids and ...
Breaking Biology News(10 mins):BUSM's Michael F. Holick receives American Society for Bone and Mineral Research award 2New simple setup for X-ray phase contrast 2
... After the completion of a draft sequence of the Chinese ... in 2002, CAS researchers have recently finished the fine map ... recent issue of PLoS Biology (Vol.3 Issue 2, 2005), scientists ... "much improved, near-complete genome analysis of the indica and japonica ...
... provoke allergic reactions? A new study in the February ... some of the blame on bioactive molecules that are ... and cause them to launch a typical allergy-promoting immune ... more of these allergy-provoking compounds than do pollen from ...
... widely-used class of drugs that keep the HIV-virus infection ... lethal heart rhythm disturbances in some patients. The finding ... edition of The Lancet. , In collaboration with ... Center, Minneapolis; the University of Minnesota, Minneapolis, and University ...
Cached Biology News:Whole genome fine map of rice completed 2HIV Patients May Be at Risk of Heart Problems When Taking Protease Inhibitor Drugs 2HIV Patients May Be at Risk of Heart Problems When Taking Protease Inhibitor Drugs 3
This issue concentrated on the purification of proteins for specific purposes (i.e. large-scale purification, purification for therapeutic use, etc.)...
Edited by D.M. Gersten (1996) Provides comprehensive descriptions of the techniques available for analytical gel electrophoresis of proteins and offers clear step-by-step protocols for all of the m...
... M. Walker (1996) • This book ... for studying proteins and peptides. Each ... step-by-step instruction, timesaving troubleshooting tips, alternative ... of reagents and suppliers. Topics covered ...
...
Biology Products: