Navigation Links
Principles and applications of electrical cell fusion

Kurt Lucas, Eppendorf AG

Significance of cell fusion

Electrical cell fusion is an essential step in some of the most innovative methods in modern biology, such as the production of monoclonal antibodies, the cloning of mammals, and vaccination against cancer. Compared to the chemically induced cell fusion via polyethylene glycol (PEG), electrical cell fusion is a highly efficient method.
The process was discovered in 1978 by Zimmermann [1-3], who has initiated ongoing development ever since.

The principle of electrical cell fusion

First, the cells are brought into very close contact via dielectrophoresis. Unlike electrophoresis, in which direct current is applied in order to move molecules, dielectrophoresis uses highfrequency alternating current. In particles such as living cells, dipoles are induced which cause cells to align in such a way that they resemble a string of pearls which are in very close contact with each other.

A very short high-voltage pulse is then applied, which causes permeation of the cell membrane and the subsequent combining of the membranes. The cells then fuse. In order to stabilize the process, alternating voltage is then applied for a brief period.

The resulting formation is described as a heterokaryon because, although the outer cell membrane has fused, two or more cell nuclei are still present. The cell nuclei also fuse at a later point within the cell. In most cases, this results in a drastic reduction in the number of chromosomes in the nucleus.


'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Principles and applications of electrical cell fusion
2. Principles of Curve Fitting for Multiplex Sandwich Immunoassays, Rev B
3. An Assessment of the Eppendorf twin.tec plate performance in PCR* applications
4. Suggested applications for reverse transcriptases
5. Self-Drying Vacuum Pumps... the solution for applications producing condensation
6. A new wide field-of-view confocal imaging system and its applications in drug discovery and pathology
7. Production of Hybridomas by Electrofusion
8. Monitoring fluorescence resonance energy transfer (FRET) between GFP fusions in lysates of the yeast Saccharomyces cerevisiae using the Varian Cary Eclipse
9. Gram Quantities of MAb Produced with Simple Bioreactor in Serum-Free Perfusion Culture Replacing Ascitic Fluid Production
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Principles and applications electrical cell fusion

(Date:9/30/2014)... 30, 2014 CORD:USE Cord Blood Bank – ... it has entered into an Equity and Exclusive Services ... licensed a series of patented technologies for the isolation of ... applications.  Tianhe is performing Phase I/II clinical trials in ... using Tianhe,s Stem Cell Educator Therapy showing ...
(Date:9/30/2014)... (PRWEB) September 30, 2014 ... solutions for drug discovery and related life ... consortium has chosen Genedata Selector as its ... (NGS) data. FUNGITECT is a European consortium ... fungal diseases via individualized anti-fungal drug therapies. ...
(Date:9/30/2014)... , Sept. 30, 2014  KemPharm, Inc., ... discovery and development of proprietary new molecular entity ... Patent and Trademark Office (USPTO) issued U.S. Patent ... entitled, "Benzoic Acid, Benzoic Acid Derivatives and Heteroaryl ... Making and Use Thereof." The patent, ...
(Date:9/30/2014)... , Sept. 30, 2014 FemCap Inc., ... health announces its receipt of a patent No: US, ... cup has also received FDA clearance in the U.S., ... the CE in Europe. This patent is related to its ... this issuance, the company is releasing FemmyCycle Teen, a ...
Breaking Biology Technology:CORD:USE is Pleased to Announce it has Partnered with Tianhe Stem Cell Biotechnologies, Developer of Cord Blood Stem Cell Therapy Showing Promising Results in Clinical Trials for the Treatment of Diabetes and Autoimmune Diseases 2CORD:USE is Pleased to Announce it has Partnered with Tianhe Stem Cell Biotechnologies, Developer of Cord Blood Stem Cell Therapy Showing Promising Results in Clinical Trials for the Treatment of Diabetes and Autoimmune Diseases 3CORD:USE is Pleased to Announce it has Partnered with Tianhe Stem Cell Biotechnologies, Developer of Cord Blood Stem Cell Therapy Showing Promising Results in Clinical Trials for the Treatment of Diabetes and Autoimmune Diseases 4FUNGITECT Consortium Adopts Genedata Selector as Data Analysis Platform for Anti-fungal R 2FUNGITECT Consortium Adopts Genedata Selector as Data Analysis Platform for Anti-fungal R 3KemPharm, Inc. Receives Patent from the USPTO for Novel Prodrug of Hydromorphone, KP511 2The FemmyCycle 2
... Beckman Coulter,Inc. (NYSE: BEC ), a ... automate, and innovate complex biomedical testing,announced today that ... present at the 19th Annual Piper Jaffray Healthcare,Conference ... (ET). (Logo: http://www.newscom.com/cgi-bin/prnh/20031202/BECLOGO ) The ...
... Highlighted by the,introduction of the IQQA(R) Enterprise ... radiology imaging market with its universal enterprise,solution, ... of 2D/3D/4D advanced,clinical applications on existing PACS ... code-level integration. Available on the,IQQA(R) Enterprise Engine ...
... Saint Louis University,(SLU) recognized St. Louis-based Sigma-Aldrich(TM) (Nasdaq: ... life science and high technology company,s,recent $1 million ... largest,in Sigma-Aldrich history, comes in the form of ... by the university,s medical school research,teams. (Photo: ...
Cached Biology Technology:Beckman Coulter to Present at the 19th Annual Piper Jaffray Healthcare Conference 2EDDA Technology Introduces Universal Enterprise Solution - Enabling 2D/3D/4D Advanced Clinical Applications on Existing PACS Workstations 2EDDA Technology Introduces Universal Enterprise Solution - Enabling 2D/3D/4D Advanced Clinical Applications on Existing PACS Workstations 3Saint Louis University Honors Sigma-Aldrich Corp. for $1 Million Donation 2
(Date:9/30/2014)... Here,s some hope: A new research report published in ... , explains how scientists developed a synthetic version ... embryo development in human and mouse eggs similar to ... cell during fertilization. , "We believe that the results ... our understanding of human fertilization by providing a precise ...
(Date:9/30/2014)... 30, 2014  Spectra Automation, a trusted ... biotech and power generation industries, announced today ... Manager, an easy-to-use and cost-effective data collection, ... development laboratories. Most upstream ... analyzer systems. Accessing and consolidating data from ...
(Date:9/30/2014)... 30, 2014) People who suffer from asthma may ... control their asthma besides properly taking medications and avoiding ... in the Annals of Allergy, Asthma and Immunology ... Allergy, Asthma and Immunology (ACAAI), asthma sufferers can learn ... The woman described in the Annals article improved her ...
Breaking Biology News(10 mins):Synthetic sperm protein raises the chance for successful in vitro fertilization 2Spectra Automation Introduces RECONN Bioprocess Manager 2Asthma symptoms kicking up? Check your exposure to air pollution 2
... A new study, supported in part by the National Heart, ... Health, suggests that a combination of mobile technology and remote ... behavior in adults. The study focused on the best way ... appear Monday, May 28, in the Archives of Internal ...
... A study led by researchers from McGill University provides ... heart a finding that promises to contribute to the ... heart tissue. The research, published in the Proceedings ... the muscle fibers in the heart wall are locally arranged ...
... getting stronger or weaker? Right now, it,s hard to know. ... this medical challenge by developing and applying a technique that ... technique was more sensitive in detecting bone loss than the ... it may find use in clinical settings, and could pave ...
Cached Biology News:NIH-funded study examines use of mobile technology to improve diet and physical activity behavior 2Study provides new insights into structure of heart muscle fibers 2Earlier detection of bone loss may be in future 2Earlier detection of bone loss may be in future 3Earlier detection of bone loss may be in future 4
... units/µL ,• High specific activity and processivity ... 'A' overhang ,• Processes ,A ... activity in primer extension and other molecular ... the enzyme, each lot has been functionally ...
... Recombinant DNA polymerase from Thermus aquaticus ... DNA contamination; 1 tube of 250 units ... LD (Low DNA), is the same enzyme ... purified through a proprietary separation process to ...
Our original highly-purified, robust Taq Polymerase which delivers consistently high yields and minimal background...
Taq DNA polymerase, 10,000U...
Biology Products: