Navigation Links
Primer design

RT-PCR amplification of a particular mRNA sequence requires two PCR primers that are specific for that mRNA sequence. The primer design should also allow differentiation between the amplified product of cDNA and an amplified product derived from contaminating genomic DNA. There are two approaches to designing the required primers (see figure below).

Make primers that anneal to sequences in exons on both sides of an intron (Panel 1). With these primers, any product amplified from genomic DNA will be much larger than a product amplified from intronless mRNA.

Make primers that span exon/exon boundaries on the mRNA (Panel 2). Such primers should not amplify genomic DNA.


'"/>

Source:


Page: All 1

Related biology technology :

1. Control RT-PCR Primers for Human Gene Transcripts with Varying Abundance
2. RT-PCR Primer Sets for Human and Mouse Mismatch Repair Genes
3. Gene-Specific Primers for NMDA Receptor Subunits
4. RT-PCR Primers for the Study of Apoptosis
5. New PCR Primers for Human Diseases
6. Amplification of Epstein-Barr Virus Exon C with Difficult Primers Using Eppendorf Mastercycler gradient
7. General Notes on Primer Design in PCR*
8. Human t-PA Control Primer Set
9. Primer Walking
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Primer design

(Date:5/29/2015)... 29, 2015 Translation is frequently listed as ... a good reason--if used in a way that is right ... of a business product or service offering. , Here ... a positive effect on a company's bottom line: , 1.    Attract ... in the world can use, why not offer it globally? ...
(Date:5/28/2015)... May 28, 2015 ... has announced the addition of Jain ... Technologies, Markets and Companies"  to their ... report describes and evaluates animal biotechnology ... and pharmaceuticals as well as improvement ...
(Date:5/28/2015)... May 27, 2015 Research and Markets( ... PharmaBiotech,s new report "Neuroprotection - Drugs, Markets ... This report describes the role of neuroprotection in ... the nervous system as well as in chronic ... the underlying mechanisms of damage to neural tissues ...
(Date:5/28/2015)... McKinney, Texas (PRWEB) May 28, 2015 ... products for use in applications such as animal ... fat and oil reduction in wastewater treatment plants ... be attending the World Pork Expo, June 3-5 ... earlier this year in a Wall Street Journal ...
Breaking Biology Technology:Three Ways Translation Can Help Businesses Grow 2Global Animal Biotechnology Report 2015 - Technologies, Markets and Companies Analysis to 2024 2Global Animal Biotechnology Report 2015 - Technologies, Markets and Companies Analysis to 2024 3Global Neuroprotection Market Report 2015-2024 - Drugs, Markets and Companies 2Global Neuroprotection Market Report 2015-2024 - Drugs, Markets and Companies 3DryLet to Showcase Patented ManureMagicâ„¢ at the 2015 World Pork Expo, June 3-5 in Des Moines, Iowa 2
... IL (October 21, 2009) A faster tool for cell ... simple tasks have won grand prizes of the 2009 Collegiate ... Fame sponsored by the Abbott Fund, the non-profit foundation of ... Patent and Trademark Office (USPTO). Harris Wang, who invented ...
... FRANCISCO, Calif., Oct. 21 iPierian, Inc., a biopharmaceutical ... and development through the application of cellular reprogramming, today ... a $3.7 million National Institutes of Health (NIH) Grand ... The grant, given over a two-year span, will be ...
... TAI,AN CITY, China, Oct. 21 ... ("China Biologic," or the "Company"),one of the leading plasma-based ... named on Forbes Magazine,s fifth annual list of,Asia,s "200 ... To assemble its Best Under ...
Cached Biology Technology:Winners announced in 2009 Collegiate Inventors Competition 2Winners announced in 2009 Collegiate Inventors Competition 3Winners announced in 2009 Collegiate Inventors Competition 4Winners announced in 2009 Collegiate Inventors Competition 5iPierian to Collaborate With Johns Hopkins University on $3.7 Million NIH Grand Opportunities Grant 2China Biologic Named to Forbes Magazine's Fifth Annual List of Asia's '200 Best Under a Billion' 2China Biologic Named to Forbes Magazine's Fifth Annual List of Asia's '200 Best Under a Billion' 3
(Date:5/11/2015)... , May 11, 2015 Curemark LLC, a ... start of a new Phase III double blind, randomized, ... novel formulation, CM-AT, on all children ages 3-8 with ... FDA Phase III double blinded clinical trial for CM-AT ... levels of the digestive enzyme chymotrypsin. This new trial ...
(Date:5/7/2015)... , May 7, 2015 ... fingerprint sensors, FPC1022 and FPC1035, FPC,s smallest touch ... FPC1035 are mainly considered for integration on the ... gives smartphone OEMs increased possibilities to integrate touch ... decreased size also improves possibilities for module manufacturers ...
(Date:4/27/2015)... more than four decades, AUVSI,s Unmanned Systems ... event for the unmanned land, sea, and air industries. ... local, national, or trade news organization interested in gathering ... applications of unmanned technologies. The conference will explore the ... and how it will soon impact the lives of ...
Breaking Biology News(10 mins):Curemark, LLC, Launches New Phase III Trial in Expanded Population of Children with Autism 2FPC Introduces its Smallest Touch Fingerprint Sensors to Date 2UNMANNED SYSTEMS 2015: Largest International Conference and Trade Show for the Drone and Unmanned Technology Industry 2UNMANNED SYSTEMS 2015: Largest International Conference and Trade Show for the Drone and Unmanned Technology Industry 3UNMANNED SYSTEMS 2015: Largest International Conference and Trade Show for the Drone and Unmanned Technology Industry 4UNMANNED SYSTEMS 2015: Largest International Conference and Trade Show for the Drone and Unmanned Technology Industry 5
... JUPITER, FL, November 9, 2010 For Immediate Release ... Research Institute have identified for the first time a ... that controls the body,s natural 24-hour physiological cycle. These ... for jet lag, shift work, and sleep disturbances, but ...
... commonly used to treat diabetes may also retard the ... disorder, polycystic kidney disease. PKD does not discriminate by ... worldwide. Researchers from the schools of Science ... from the Mayo Clinic report this month in the ...
... Soil is no longer necessary for growing potatoes, at ... Company) is undertaking a research project, together with Neiker-Tecnalia ... producing tubers in air, by means of a novel ... is a pioneering experience in Spain, given that never ...
Cached Biology News:Scripps Research scientists identify new mechanism regulating daily biological rhythms 2Scripps Research scientists identify new mechanism regulating daily biological rhythms 3Common diabetes drug may halt growth of cysts in polycystic kidney disease 2Newco produces seed potatoes in the open air 2Newco produces seed potatoes in the open air 3
... The EC570-90 is a compact, lightweight, high ... 500 volts or 2500 milliamps. It is an ... RNA submarine gels. It is also ideal for ... time can be set to automatically end a ...
Rat Hepatocytes (RH) (>3,000,000 cells)...
QCPN...
Rat Aortic Smooth Muscle Cells (RAOSMC) (>500,000 cells)...
Biology Products: