Navigation Links
Primer design

RT-PCR amplification of a particular mRNA sequence requires two PCR primers that are specific for that mRNA sequence. The primer design should also allow differentiation between the amplified product of cDNA and an amplified product derived from contaminating genomic DNA. There are two approaches to designing the required primers (see figure below).

Make primers that anneal to sequences in exons on both sides of an intron (Panel 1). With these primers, any product amplified from genomic DNA will be much larger than a product amplified from intronless mRNA.

Make primers that span exon/exon boundaries on the mRNA (Panel 2). Such primers should not amplify genomic DNA.


'"/>

Source:


Page: All 1

Related biology technology :

1. Control RT-PCR Primers for Human Gene Transcripts with Varying Abundance
2. RT-PCR Primer Sets for Human and Mouse Mismatch Repair Genes
3. Gene-Specific Primers for NMDA Receptor Subunits
4. RT-PCR Primers for the Study of Apoptosis
5. New PCR Primers for Human Diseases
6. Amplification of Epstein-Barr Virus Exon C with Difficult Primers Using Eppendorf Mastercycler gradient
7. General Notes on Primer Design in PCR*
8. Human t-PA Control Primer Set
9. Primer Walking
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:4/28/2016)... ... April 28, 2016 , ... Next week on May 5 ... first-in-class technologies for tissue stem cell counting and expansion to gene-editing scientists and ... & CRISPR-based Genome Engineering in Burlington, Massachusetts. , The attention of most gene-editing ...
(Date:4/27/2016)... , ... April 27, 2016 , ... The Pittcon Organizing ... 2019. Chuck has been a volunteer member of Committee since 1987. Since then, ... board of directors and treasurer and was chairman for both the program and exposition ...
(Date:4/27/2016)... , ... April 27, 2016 , ... ... Jon Clark has joined the company as an Expert Consultant. Mr. Clark ... industry collaborations and managing the development of small molecule monographs based on analytical ...
(Date:4/27/2016)... , April 27, 2016 MedDay, ... disorders, today announces the appointment of Catherine Moukheibir as Chairman ... MedDay,s previous Chairman, Jean Jacques Garaud , who contributed ... change is effective immediately. Catherine started her career ... and London .  She held ...
Breaking Biology Technology:
(Date:3/21/2016)... 2016 Unique technology combines ... superior security   Xura, Inc. ... secure digital communications services, today announced it is working ... enterprise customers, particularly those in the Financial Services Sector, ... authentication within a mobile app, alongside, and in combination ...
(Date:3/14/2016)... Florida , March 14, 2016 ... the growing mobile commerce market, announces the airing of a ... channels starting the week of March 21 st .  The ... CNBC, including its popular Squawk on the Street show. ... focused on the growing mobile commerce market, announces the airing ...
(Date:3/10/2016)... , March 10, 2016   Unisys Corporation (NYSE: ... Border Protection (CBP) is testing its biometric identity solution ... Diego to help identify certain non-U.S. citizens leaving ... The test, designed to help determine the efficiency and accuracy ... in February and will run until May 2016. --> ...
Breaking Biology News(10 mins):