Navigation Links
Primer Walking

Full Clone Sequencing


Agencourt offers primer walking sequencing of PCR products, plasmid inserts or entire plasmid clones. Simply submit your template in either raw or purified form and we will return to you an assembled consensus sequence. Select from either single stranded coverage for confirmation sequencing or double stranded coverage for publication quality or clone validation purposes.


Key features of the service include:
Proprietary primer design software
High quality consensus sequencing
Sophisticated bioinformatics and reporting tools
Unparalleled sample tracking and data security


Comprehensive Service Offering
Our comprehensive primer walking and assembly service includes the following features:
Template purification using our patented SPRI chemistry
Automated primer picking using our proprietary design software
Sequencing reaction setup and thermal cycling
Dye-terminator removal using our SPRI chemistry
Sequence detection using ABI 3730xl capillary sequencers
Assembly and finishing to generate contiguous consensus sequence
Detailed reporting tools


Data Quality
Agencourt uses custom software for the successful design of oligo sequences for primer walking. Use of our SPRI DNA purification chemistry with ABI 3730xl sequencers provides superior sequencing results. Dedicated highly trained personnel use our proprietary bioinformatics pipeline for sequence assembly, data analysis, and sequence editing to generate the highest quality finished sequence. Agencourt utilizes the Bermuda Standard for finished sequence which is recognized as the gold standard in sequence quality.


Detailed Reporting Tools
For clone verification and confirmation sequencing applications, Agencourt provides detailed reporting tools to aid customers in the analysis of their sequence data. In addition to providing an assembled consensus sequence, we can provide discrepancy reports comparing sequence data to a reference sequence. The discrepancy report annotates sequence differences and amino acid changes for easy analysis.


State-of-the-Art Facility
With the capacity to sequence over 75,000 lanes per day, we can manage both large and small projects with total customer satisfaction in mind. Our highly automated pipeline integrates manufacturing principles such as statistical process control and preventive maintenance programs to assure consistent high quality results.


back to top
'"/>

Source:


Page: All 1 2

Related biology technology :

1. Control RT-PCR Primers for Human Gene Transcripts with Varying Abundance
2. RT-PCR Primer Sets for Human and Mouse Mismatch Repair Genes
3. Gene-Specific Primers for NMDA Receptor Subunits
4. RT-PCR Primers for the Study of Apoptosis
5. New PCR Primers for Human Diseases
6. Amplification of Epstein-Barr Virus Exon C with Difficult Primers Using Eppendorf Mastercycler gradient
7. General Notes on Primer Design in PCR*
8. Human t-PA Control Primer Set
9. Primer design
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:2/10/2016)... (PRWEB) , ... February 10, 2016 , ... ... attend the International Society of Pharmaceutical Engineering (ISPE) Rocky Mountain Chapter 21st Annual ... is expecting to fill more than 100 tables for its annual event, which ...
(Date:2/10/2016)... (PRWEB) , ... February 10, 2016 , ... ... now available on Microsoft Azure. On Azure, Arvados provides capabilities for managing and ... clear demand for Microsoft Azure from major institutions collecting and analyzing genomic data,” ...
(Date:2/9/2016)... ... 2016 , ... With a presidential election in November and the future of ... together over 500 top healthcare leaders for a night and day of debates and ... MBA students of the University of Pennsylvania’s Wharton School, will be held February 18 ...
(Date:2/9/2016)... ... February 09, 2016 , ... Clinovo , ... and validated Electronic Data Capture (EDC) system ClinCaptureand its new Contract Research Organization ... Coast 2016 Conference in San Mateo, California on February 10th and 11th. ...
Breaking Biology Technology:
(Date:1/27/2016)... 2016  Rite Track, Inc. a leading semiconductor equipment ... Chester, Ohio announced today the acquisition of PLUS ... in Austin, Texas , will significantly ... modifications, installations and technical support offerings for TEL Track ... commented, "PLUS has provided world class service including refurbishment, ...
(Date:1/21/2016)... --> ... report "Emotion Detection and Recognition Market by Technology (Bio-Sensors, NLP, ... Voice Recognition and Others), Services, Application Areas, End ... published by MarketsandMarkets, the global Emotion Detection and ... Billion by 2020, at a CAGR of 31.9%, ...
(Date:1/15/2016)... , Jan. 15, 2016 Recent publicized ... small to find new ways to ensure data security ... iOS and Android that ties ... biometrics, transforming it into a hardware authorization token. Customer ... swipe their fingerprint on their KodeKey enabled device to ...
Breaking Biology News(10 mins):