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Primer Walking

Full Clone Sequencing


Agencourt offers primer walking sequencing of PCR products, plasmid inserts or entire plasmid clones. Simply submit your template in either raw or purified form and we will return to you an assembled consensus sequence. Select from either single stranded coverage for confirmation sequencing or double stranded coverage for publication quality or clone validation purposes.


Key features of the service include:
Proprietary primer design software
High quality consensus sequencing
Sophisticated bioinformatics and reporting tools
Unparalleled sample tracking and data security


Comprehensive Service Offering
Our comprehensive primer walking and assembly service includes the following features:
Template purification using our patented SPRI chemistry
Automated primer picking using our proprietary design software
Sequencing reaction setup and thermal cycling
Dye-terminator removal using our SPRI chemistry
Sequence detection using ABI 3730xl capillary sequencers
Assembly and finishing to generate contiguous consensus sequence
Detailed reporting tools


Data Quality
Agencourt uses custom software for the successful design of oligo sequences for primer walking. Use of our SPRI DNA purification chemistry with ABI 3730xl sequencers provides superior sequencing results. Dedicated highly trained personnel use our proprietary bioinformatics pipeline for sequence assembly, data analysis, and sequence editing to generate the highest quality finished sequence. Agencourt utilizes the Bermuda Standard for finished sequence which is recognized as the gold standard in sequence quality.


Detailed Reporting Tools
For clone verification and confirmation sequencing applications, Agencourt provides detailed reporting tools to aid customers in the analysis of their sequence data. In addition to providing an assembled consensus sequence, we can provide discrepancy reports comparing sequence data to a reference sequence. The discrepancy report annotates sequence differences and amino acid changes for easy analysis.


State-of-the-Art Facility
With the capacity to sequence over 75,000 lanes per day, we can manage both large and small projects with total customer satisfaction in mind. Our highly automated pipeline integrates manufacturing principles such as statistical process control and preventive maintenance programs to assure consistent high quality results.


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