Navigation Links
Primary cells, rat heart muscle

Multiporator Transfection Protocol Protocol No. 4308 915.035 12/1999 Cell line Primary cells: Adult heart muscle cells from rat, 6 days in culture Transfection with Plasmid pCMV--galactosidase (in bidistilled H2O) Electroporation buffer Eppendorf Isoosmolar Electroporation Buffer (PI) Culture medium M199 / HEPES (2 mM carnithin, 5 mM taurine, 10 M cytosine-arabinofur.)+ 20% FCS Outgrowth medium HEPES-buffer (140 mM NaCl, 3.6 mM KCl, 1.2 mM MgSO4, 1 mM CaCl2, 11 mM glucose, 100 U/ml penicillin, 100 g/ml streptomycin, 20 mM HEPES, pH 7.4) without phenol red + 10% FCS Cuvette Eppendorf, 2 mm gap width, 400 l Temperature RT (20-25 C) Reference Dr. Gerhild Taimor Justus-Liebig-Universitt Physiologisches Institut Aulweg 129 D-35392 Gieen
Phone +49 641 9947243 Fax +49 641 9947239 mail: gerhild.taimor@physiologie.med.uni-giessen.de
  1. Harvest the cells by collagenase treatment (0.4 mg/ml, 30 min., 37 C) and centrifuge them (for 2 minutes, 300 x g, at room temperature).
  2. Resuspend the cells in M199 / 0.5% FCS, determine the number of cells and centrifuge them (for 2 minute
    '"/>


Source:


Page: All 1 2

Related biology technology :

1. Human Primary Preadipocytes and Differentiated Adipocytes
2. Primary cells, rat heart endothel
3. Efficient Delivery of siRNAs to Human Primary Cells: Electroporation vs. Chemical Transfection
4. Deliver siRNAs Into Primary Cells
5. High Throughput siRNA Delivery In Vitro: From Cell Lines to Primary Cells
6. Electroporation of Primary Bone Marrow Cells
7. Efficient Primary Structure Elucidation of Disulfide-bridged Peptides
8. Embryonic stem cells, mouse
9. Laser Capture Microdissection of muscle fiber populations and expression analysis by RT-PCR
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Primary cells rat heart muscle

(Date:10/27/2014)... BOSTON, MA (PRWEB) October 27, 2014 ... and profit. SoundConnect , an industry leading ... for its partners and agents to deliver cloud-based ... and accelerate growth opportunities. , With SoundConnect’s ... for every twenty-five video and/or web conferencing licenses ...
(Date:10/27/2014)... 27, 2014 The report “Smart ... Traffic Management, Communication, Monitoring), and by Display (Variable ... 2019” segments the global market into various sub-segments ... also identifies the drivers and restraints for this ... , Browse 75 market tables and 37 figures ...
(Date:10/27/2014)... Pa. , Oct. 27, 2014 ... Foundation of America (CCFA) announced today the launch ... , an educational campaign designed to ... diseases (IBD) on the paired role of nutrition ... To view the multimedia assets associated with this ...
(Date:10/26/2014)... 26, 2014 The report "Data ... Network Security Equipment, WAN Optimization Appliances]: Global Advancements, ... the data center networking market into various segments ... The report also identifies the factors driving this ... it along with the future roadmaps. , Browse ...
Breaking Biology Technology:SoundConnect Unveils Q4 Partner Incentives 2Smart Highway Market by Technology, and Display Expected to Reach $27.992.0 Million by 2019- New Report by MarketsandMarkets 2Smart Highway Market by Technology, and Display Expected to Reach $27.992.0 Million by 2019- New Report by MarketsandMarkets 3Smart Highway Market by Technology, and Display Expected to Reach $27.992.0 Million by 2019- New Report by MarketsandMarkets 4Celebrity Cook Sunny Anderson Teams Up With Janssen And Crohn's & Colitis Foundation Of America To Launch Educational Campaign 2Celebrity Cook Sunny Anderson Teams Up With Janssen And Crohn's & Colitis Foundation Of America To Launch Educational Campaign 3Celebrity Cook Sunny Anderson Teams Up With Janssen And Crohn's & Colitis Foundation Of America To Launch Educational Campaign 4Data Center Networking Market by Ethernet Switches, SAN & Routers Worth $21.85 Billion by 2018 - New Report by MarketsandMarkets 2Data Center Networking Market by Ethernet Switches, SAN & Routers Worth $21.85 Billion by 2018 - New Report by MarketsandMarkets 3Data Center Networking Market by Ethernet Switches, SAN & Routers Worth $21.85 Billion by 2018 - New Report by MarketsandMarkets 4
... news item is out of step with public opinion in ... state of Wisconsin grants $1 million and loans another $1 ... UW-Madison researcher so renowned that the words "Nobel Prize" practically ... drug discovery. , ,• The National Institutes of Health, the ...
... records have led to some adoption of the technology but ... ,Then Hurricane Katrina slammed into the Gulf Coast, destroying countless ... ,Katrina, and her followup Rita, may not only have changed ... focus of debate on electronic healthcare records. It is becoming ...
... -- Research scientist Seth Dobrin, Ph.D., has been named ... Genotyping Service of the Marshfield Clinic Research Foundation. , ,He ... to problems of mental health and development of new genetic ... discoveries he made earlier in his career as well as ...
Cached Biology Technology:Stem cell bank announcement validates quality of Wisconsin research 2Stem cell bank announcement validates quality of Wisconsin research 3Katrina's destruction likely to speed up electronic healthcare records adoption 2Katrina's destruction likely to speed up electronic healthcare records adoption 3Katrina's destruction likely to speed up electronic healthcare records adoption 4
(Date:10/28/2014)... differences in moving activity in a novel environment ... angling, according to an experimental study completed at ... Game and Fisheries Research Institute. The study used ... and authentic angling trials to analyse if behaviours ... reared in traditional and enriched hatchery rearing environments. ...
(Date:10/28/2014)... and citrus fruits and juices are associated with a ... research from the University of East Anglia (UEA). ... foods containing flavonols and flavanones (both subclasses of dietary ... cancer, the fifth-leading cause of cancer death among women. ... of 171,940 women aged between 25 and 55 for ...
(Date:10/27/2014)... are an indigenous species of the West Antarctic Peninsula ... Earth. Since 1950, the average annual temperature in the ... and 6 degrees Celsius during winter. , As ... dry, polar system to a warmer, sub-polar system with ... a connection between local weather conditions and the weight ...
Breaking Biology News(10 mins):Fish 'personality' linked to vulnerability to angling 2Tea and citrus products could lower ovarian cancer risk, new UEA research finds 2University of Delaware study connects penguin chick weights to local weather conditions 2University of Delaware study connects penguin chick weights to local weather conditions 3
... they used fluorescent molecules to "tag" DNA and monitor a ... rearranging genetic material in some types of cells. ... light on how DNA loops form, but also might be ... shuffle genetic material, such as HIV. Until now, scientists ...
... more DNA regions linked to type 2 diabetes have been ... to over 60. The study provides a fuller picture ... with some clear patterns emerging. The international team, ... Institute of Harvard and MIT, and the University of Michigan, ...
... affordable and accessible, we need to pay more attention to ... and the fact that we don,t yet understand what ... Hastings Center Report . The authors are current or former ... Most analyses of the ethical issues raised by whole ...
Cached Biology News:Scientists use light to 'tag and track' genetic processes 210 new diabetes gene links offer picture of biology underlying disease 210 new diabetes gene links offer picture of biology underlying disease 3Prenatal whole genome sequencing: Just because we can, should we? 2Prenatal whole genome sequencing: Just because we can, should we? 3
Mouse monoclonal antibody raised against a partial recombinant FBXO24. NCBI Entrez Gene ID = FBXO24...
Mouse monoclonal antibody raised against a partial recombinant CALCOCO2. NCBI Entrez Gene ID = CALCOCO2...
Mouse monoclonal to Casein Kinase 2 beta ( Abpromise for all tested applications). entrezGeneID: 1460 SwissProtID: P13862...
...
Biology Products: