Navigation Links
Preserve Samples for RNA Expression Microarrays RNAlater Around the World


RNAlater protects cellular RNA within specimens during sample collection and during shipping. Whether you are in the lab, in the OR, or out in the field, you can use this aqueous, non-toxic Tissue Collection:RNA Stabilization Solution from ambient temperatures down to -20C.

Characterization of disease mechanisms using high throughput technologies often involves collaborations with a core facility for processing samples and data. Tissue procurement is a critical first step in the path to quantifying gene expression. Standardization of this step enables more accurate expression profile comparisons--both within a study and across published reports. The two studies described below demonstrate how RNAlater can protect RNA expression profiles within samples destined for array analyses without requiring specialized equipment (e.g. liquid nitrogen and associated containers) or handling (e.g. RNAlater is non-toxic and samples are stable at ambient temperatures).


Microarrays with Human Uterine Tissue

To expedite transfer of discoveries in genomic medicine to clinical care, Dr. George Mutter and colleagues conducted a comprehensive study to assess the impact of tissue sample source, tissue processing protocol, and random noise on microarray analyses [1]. Single uterine myometrium biopsies from randomly selected patients with benign uterine disease were subdivided into eight aliquots. Duplicate samples were either homogenized immediately, flash frozen in liquid nitrogen and stored at -80C for 48 hours, or immersed in RNAlater at room temp for 24 or 72 hours.

In the expression profiles obtained from Affymetrix HG-U133A arrays, samples from different patients had the greatest variability (measured by mixed model ANOVA, mean square error for genes with expression values >=100). Method of tissue collection and replicate samples from the same patient had the least variation, meaning microarray data obtained from routine repeat specimens from the same patient stored for 24 or 72 hours in RNAlater were not significantly different from those obtained when biopsies were fresh or flash frozen. In short, the variation in results observed between replicate samples from different tissue processing protocols was no greater than the variation between replicate samples from the same processing protocols. Thus, in this experiment, tissue could be stored in RNAlater at room temperature for up to three days without introducing unwanted variability to the gene expression analysis. These conclusions were further supported by data obtained from examining profiles of nine gene subgroups that consisted of multiple, functionally related gene classes.


Microarrays With Mouse Liver Tissue

Ambion scientists have a lso tested whether sample storage affects microarray expression profiling. Duplicate mouse liver samples were dissected and either frozen at -80C for one week or stored in RNAlater at room temperature for 24 hours, 4C for one week, or -20C for one week. RNA was isolated (RiboPure, Ambion), linearly amplified (MessageAmp II aRNA Kit, Ambion), and labeled with biotin-UTP before hybridization to an Affymetrix MG 430A 2.0 array. The mean standard deviation for percent present calls for all samples was 59.4 1.1, and the correlation between samples was excellent (range, R=0.988 to R=0.996). As a representative example, Figure 1 is a scatter plot summarizing the overall results when expression levels from samples stored in RNAlater for 24 hours were compared to that of samples frozen at -80C.

Figure 1. Scatter Plot of Gene Expression Levels from Samples Stored in RNA later vs Samples Frozen at -80C. Duplicate mouse liver samples were dissected and either frozen at -80C for one week or stored in RNAlater at room temperature for 24 hours, 4C for one week, or -20C for one week. RNA was isolated (RiboPure, Ambion), and 1 g total RNA was linearly amplified (MessageAmp II aRNA Kit, Ambion). After a four hour in vitro transcription to amplify and label samples with biotin-16-UTP, the samples were hybridized to Affymetrix MG 430A 2.0 arrays (22,690 genes). This scatter plot summarizes the overall results when expression levels from samples stored in RNAlater for 24 hours were compared to that of samples frozen at -80C.


For more accurate gene expression profiling data, RNAlater can be used to stabilize samples during tissue collection and storage. The convenience and ease- of-use makes RNAlater ideal for preserving clinical and research samples that will be processed for microarray analyses.

Do you have an interesting story about an RNAlater application? We'd love to hear about it! If we include your story in a future issue of TechNotes, we will send you a free t-shirt and more RNAlater. Please email TechNotes@ambion.com.


Scientific Contributors
Anton Zimmerman Ambion, Inc.


back to top


Ordering Information
Cat# Product Name Size 7020 RNAlater 100 ml 7021 RNAlater 500 ml 7022 RNAlater 50 x 1.5 ml 7023 RNAlater 20 x 5 ml 7024 RNAlater 250 ml
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. RNAlater Preserves Bacterial Gene Expression Profiles for Array Analysis
2. Genomic DNA Preparation from RNAlater Preserved Tissues
3. Quantitation of the Protein MIA as a Marker for Chondrocytes in Research Samples
4. Isolate High Quality Total RNA from LCM Samples Suitable for Microarray and qRT-PCR Analysis
5. RNA from LCM Samples
6. Robust High Throughput RNA Isolation from Blood Samples
7. RNAlater Around the World: The Art of Processing Difficult Samples
8. Working With Laser Capture Microdissection Samples
9. Bio-Plex Cytokine Immunoassays and ELISA: Comparison of Two Methodologies in Testing Samples From Asthmatic and Healthy Children, Rev A
10. Analysis of Bacterial Assemblage Genetic Diversity in Environmental Samples Using the DCode System
11. Fast Multi-Residue Pesticide Analysis in Soil and Vegetable Samples
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:2/10/2016)... ASAE is introducing a hybrid membership model ... the option of joining or renewing through an organizational ... staff size, every employee in any size association or ... all available member benefits.   John H. ... options will allow organizations of any size and their ...
(Date:2/10/2016)... , Feb. 10, 2016  Allergan plc (NYSE: ... announced that Brent Saunders , Allergan,s CEO and ... a fireside chat session at the RBC Capital Markets ... p.m. ET at The New York Palace Hotel in ... will be webcast live and can be accessed on ...
(Date:2/10/2016)... ... February 10, 2016 , ... HOLLOWAY AMERICA, ... Pharmaceutical Engineering (ISPE) Rocky Mountain Chapter 21st Annual Vendor Exhibition on Thursday, February ... than 100 tables for its annual event, which will run from 3:00 p.m. ...
(Date:2/10/2016)... Mateo, CA (PRWEB) , ... February 10, 2016 ... ... of Multiplex Testing (PROMPT), a research registry built on the secure online PatientCrossroads ... September 2014. More than 1,600 participants have joined the PROMPT study, which seeks ...
Breaking Biology Technology:
(Date:1/21/2016)... --> ... report "Emotion Detection and Recognition Market by Technology (Bio-Sensors, NLP, ... Voice Recognition and Others), Services, Application Areas, End ... published by MarketsandMarkets, the global Emotion Detection and ... Billion by 2020, at a CAGR of 31.9%, ...
(Date:1/18/2016)... SAN JOSE, Calif. , Jan. 18, 2016 ... storage security software that simplifies the use and ... a technology and go-to-market partnership with American Cyber.  ... "American Cyber brings extensive experience leading transformational C4ISR ... missions implementing and integrating the latest proven technology ...
(Date:1/11/2016)... 11, 2016 Synaptics Incorporated (NASDAQ: SYNA ... announced that its ClearPad ® TouchView ™ ... two separate categories in the 8 th Annual ... Technology Breakthrough. The Synaptics ® TDDI solution enables ... chain, thinner devices, brighter displays and borderless designs. ...
Breaking Biology News(10 mins):