Complete Kit for Probe Preparation
The kit includes enough reagents to prepare 30 dsDNA templates and to perform 30 in vitro transcription reactions. Each template construction reaction supplies sufficient template for up to 20 transcription reactions. The kit also includes a functional positive control, DNase I to remove residual DNA template, and Probe Elution Buffer to gel purify RNA transcripts.
Figure 1. miR-16
and miR-22 Expression in Mouse Brain. In
situ hybridization was performed on coronal mouse forebrain sections.
In situ hybridized cells in the mouse brain cortex (A,B,D,E; 20X magnification)
and the head of the caudate nucleus (C,F; 40X magnification) are indicated
by arrows. Nonisotopically labeled probes were prepared with Ambion's
Kit (1.5 kb probe) or with the mirVana miRN