Navigation Links
Prepare siRNA and miRNA Probes in Just 1 Hour

mirVana miRNA Probe Construction Kit


Prepare your probe in less than 1 hour

Ideal for siRNA/miRNA studies

Synthesize high specific activity RNA probes for RPA, Northern, Southern, or in situ hybridization

No cloning required


With the mirVana miRNA Probe Construction Kit, short (<100 nt) radiolabeled or nonisotopically labeled RNA probes can be generated in less than 1 hour. These probes are ideal for the investigation of a variety of small RNAs. Radiolabeled probes prepared with the mirVana miRNA Probe Construction Kit have been successfully used for the detection of small nuclear RNA (snRNA), small interfering RNA (siRNA), micro RNA (miRNA), and mRNA by RPAs, Northerns and in situ hybridization (see Detecting miRNA & siRNA). Nonisotopically labeled probes generated with the kit have also been used to study the distribution of miRNA or mRNA in tissues by in situ hybridization (See Analysis of miRNA Distribution in Mouse Brain, right).


RNA Probe Synthesis Made Fast, Simple and Inexpensive
The mirVana miRNA Probe Construction Kit includes reagents for preparation of in vitro transcription templates as well as reagents for in vitro transcription. For template preparation, all you n eed to supply is a short, inexpensive DNA oligonucleotide specific for the target of interest that also includes an 8 base sequence complementary to the 3' end of the T7 promoter primer included in the kit. This oligonucleotide is annealed to the provided T7 promoter primer, and a 30 minute fill-in reaction with Klenow fragment generates the double-stranded transcription template. The resulting template is simply mixed with the provided T7 RNA Polymerase, rNTPs, and Transcription Buffer along with your choice of labeled rNTP. The reaction is then incubated for as little as 10 minutes at 37C to generate labeled probe .


Complete Kit for Probe Preparation
The kit includes enough reagents to prepare 30 dsDNA templates and to perform 30 in vitro transcription reactions. Each template construction reaction supplies sufficient template for up to 20 transcription reactions. The kit also includes a functional positive control, DNase I to remove residual DNA template, and Probe Elution Buffer to gel purify RNA transcripts.

Figure 1. miR-16 and miR-22 Expression in Mouse Brain. In situ hybridization was performed on coronal mouse forebrain sections. In situ hybridized cells in the mouse brain cortex (A,B,D,E; 20X magnification) and the head of the caudate nucleus (C,F; 40X magnification) are indicated by arrows. Nonisotopically labeled probes were prepared with Ambion's MAXIscript Kit (1.5 kb probe) or with the mirVana miRN A Probe Construction Kit (32 nt long probes, 22 nt target specific sequence).


back to top


Ordering Information
Cat# Product Name Size 1550 mirVana miRNA Probe Construction Kit 30 rxns
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Rapidly Prepare Probes and Markers for Small RNA Analysis
2. InstaGene Matrix: Prepare DNA Templates for PCR With No Phenol/Chloroform Extractions and No Deproteinization Steps, Rev C
3. Custom and library siRNA for efficient gene silencing
4. Custom and library siRNA for efficient gene silencing
5. Cancer siRNA Oligo Set Version 1.0
6. Library siRNA
7. Custom siRNA Oligo Synthesis Service
8. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
9. Quantification of siRNA Silencing Efficiency Using the LightCycler System
10. Housekeeping Genes: Universal Positive Controls in siRNA Knockdown Experiments
11. Confirming gene silencing mechanism by pGFP/GFP22 siRNA co-transfection
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:5/27/2016)... ... , ... PBI-Gordon Corporation is pleased to announce Dave Loecke has accepted the ... with PBI-Gordon, Dave has served in a wide variety of roles. His most recent ... launch of many of PBI-Gordon’s most successful products. , “Dave has been essential to ...
(Date:5/27/2016)... 2016 At present, the Biotech sphere ... space know that volatility is what makes this industry interesting ... Synta Pharmaceuticals Corp. (NASDAQ: SNTA ), CTI BioPharma ... LPTN ), and Heat Biologics Inc. (NASDAQ: ... technical alerts for these stocks at: http://www.activewallst.com/register/ ...
(Date:5/26/2016)... -- Q BioMed Inc. (OTCQB: QBIO), a biotechnology ... presenter at the 5th Annual Marcum MicroCap Conference on Thursday, ... at the Grand Hyatt Hotel. The Company,s ... is scheduled to begin at 11a.m ET in the Broadway ... developments and outline milestones for the balance of 2016 and ...
(Date:5/26/2016)... ... May 26, 2016 , ... Kinder Scientific (KinderScientific.com), ... developments that position the Company for the future. Kinder Scientific announces restructured ... Kinghorn has been appointed Chairman of the Board, Curtis D. Kinghorn has been ...
Breaking Biology Technology:
(Date:3/21/2016)... , March 22, 2016 ... recognition with passcodes for superior security   ... a leading provider of secure digital communications services, today ... biometric technology and offer enterprise customers, particularly those in ... facial recognition and voice authentication within a mobile app, ...
(Date:3/15/2016)... March 15, 2016 --> ... by Transparency Market Research "Digital Door Lock Systems Market - ... - 2023," the global digital door lock systems market in ... 2014 and is forecast to grow at a CAGR of ... small and medium enterprises (MSMEs) across the world and high ...
(Date:3/14/2016)... March 14, 2016 http://www.apimages.com ... --> - Renvoi : image disponible via ... --> --> DERMALOG, le ... de nouveaux lecteurs d,empreintes digitales pour l,enregistrement des ... sera utilisé pour produire des cartes d,identité aux ...
Breaking Biology News(10 mins):